Food pathogens Detection By Real-Time PCR Benefit from ADIAFOOD technology! ® Release your products within 24 hours! 2h30 after enrichment Ready-to-use, robust and automated Immediate implementation Flexible analysis throughput 1 to 88 samples per run Rapid Simple Reliable Adiagene and AES CHEMUNEX Canada, well known experts in microbiology, have developed kits specially designed to suit food industry needs. Specializing in industrial microbiology for more than 25 years, AES Laboratoire group offers a real-time PCR solution for food pathogens detection, and also provides a comprehensive range of services (training, support and consulting). ADIAFOOD® Kits ADIAFOOD ® Kits Protocol Analysis Rapid ADIAFOOD® kits have been designed to simplify analysis! > One single amplification protocol for all pathogens > Simultaneous analysis of several pathogens is possible > Rapid results: all ADIAFOOD® kits deliver results within 24 hours! Enrichment Listeria monocytogenes 18h Listeria spp 18h Salmonella 18h E. coli O157 and O157:H7 18h Salmonella & E. coli O157:H7 in raw meat PCR 2h30 < 24h Full kit: Extraction + PCR 8h > Also available: Campylobacter (detection and quantification) and Staphylococcus aureus kits Easy 3 step method Easy 3 step method > Simple and automated extraction (96 well microplate) > Need few handling: the thermocycler manages DNA extraction, PCR amplification and results analysis Enrichment Extraction Detection Enrichment 8 to 18 hours Extraction < 30 minutes Amplification and detection 2 hours ADIAFOOD® technology ADIAFOOD ® technolog y Reliability Accuracy Interpretation Results reliability • ADIAFOOD® technology uses 2 specificity levels (specific probes and primers) for an accurate identification of the tested pathogen, avoiding false positive results • Amplification internal control is included (duplex kits) to avoid false negative results • Controls included Analysis throughput ADIAFOOD® kits suit to your analysis throughput with 2 formats: > Microplates: ideal for a high number of tests = up to 88 samples per plate > Flexiwells: adapt your run to your analysis (numbers of samples and tested pathogens) = 8 samples per flexiwells Several pathogens can be tested on the same microplate! Open platform The Stratagene thermocycler is an opened platform that can be used with other PCR kits (ex: Legionella ADIACONTROL® kits) Automated results interpretation via Sentinel® software > Follow-up of each analysis step > Data management tool > Analysis certificate delivered per microplate or per sample > LIMS connexion Automated results analysis with Sentinel® Software: no curve interpretation! ADIAFOOD® services ADIAFOOD ® services Training Support Service Benefit from a taylor-made PCR solution! • Training: ADIAGENE and AES CHEMUNEX are registered as official training facilities • Support in laboratory organisation (flow management, drafting plans...) • Hotline Expertise in molecular biology Legionella ADIACONTROL® Food pathogens ADIAFOOD® Veterinary pathogens www.aeschemunex.com AES ChEmunEx uK Endeavour house 3rd floor • London Stansted Airport Essex • CM24 1SJ Tel.: +44 (0)1279 666 356 Fax: +44 (0)1279 666 357 [email protected] AES ChEmunEx Germany Wilhelmstraße 49 D-74918 Angelbachtal Tel.: +49(0)7265 9179 10 Fax: +49(0)7265 9179 15 [email protected] AES ChEmunEx France (headquarters) Rue maryse Bastié Ker Lann CS17219 35172 Bruz cedex Tel.: +33 (0) 2 2350 1212 Fax: +33 (0) 2 2350 1205 [email protected] AES ChEmunEx Benelux Schumacherstr. 12 D-76275 Ettlingen Tel.: +49 151 1160 4149 Fax: +49 7243 718093 AES ChEmunEx France Immeuble Paryseine 3, allée de la Seine F-94854 Ivry-sur-Seine cedex Tel.: +33(0)1 4959 2000 Fax: +33(o)1 4959 2001 [email protected] AES ChEmunEx, Inc uSA 1 Corporate Drive Cranbury nJ 08512 Tel.: +1 609 235 9272 Fax: +1 609 235 9298 [email protected] www.adiagene.com AES ChEmunEx Spain Pol. Ind. Santa margarida II C/ Albert Einstein, 44 08223 TERRASSA (Barcelona) Tel.: +34 93 785 36 27 Fax: +34 93 784 72 63 [email protected] AES ChEmunEx Italy Via Panà, 56/b 35027 nOVEnTA PADOVAnA (PD) Tel.: +39 049 87 05 804 Fax: +39 049 87 09 691 [email protected] 38, rue de Paris 22000 St-Brieuc • France Tel.: (33) 02 96 68 40 20 Fax: (33) 02 96 68 37 93 [email protected] © Masson Communication 2008 • DA280A ADIAVET®
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