Food pathogens Detection

Food pathogens Detection
By Real-Time PCR
Benefit from ADIAFOOD technology!
®
Release your products
within 24 hours!
2h30 after enrichment
Ready-to-use,
robust and automated
Immediate implementation
Flexible analysis
throughput
1 to 88 samples per run
Rapid
Simple
Reliable
Adiagene and AES CHEMUNEX Canada, well known experts in microbiology, have developed kits specially
designed to suit food industry needs. Specializing in industrial microbiology for more than 25 years,
AES Laboratoire group offers a real-time PCR solution for food pathogens detection, and also provides a
comprehensive range of services (training, support and consulting).
ADIAFOOD® Kits
ADIAFOOD ® Kits
Protocol
Analysis
Rapid
ADIAFOOD® kits have been designed to simplify analysis!
> One single amplification protocol for all pathogens
> Simultaneous analysis of several pathogens is possible
> Rapid results: all ADIAFOOD® kits deliver results within 24 hours!
Enrichment
Listeria monocytogenes
18h
Listeria spp
18h
Salmonella
18h
E. coli O157 and O157:H7
18h
Salmonella &
E. coli O157:H7
in raw meat
PCR
2h30
< 24h
Full kit:
Extraction + PCR
8h
> Also available: Campylobacter
(detection and quantification) and Staphylococcus aureus kits
Easy 3 step method
Easy 3 step method
> Simple and automated extraction (96 well microplate)
> Need few handling: the thermocycler manages DNA extraction,
PCR amplification and results analysis
Enrichment
Extraction
Detection
Enrichment
8 to 18 hours
Extraction
< 30 minutes
Amplification and detection
2 hours
ADIAFOOD® technology
ADIAFOOD ® technolog y
Reliability
Accuracy
Interpretation
Results reliability
• ADIAFOOD® technology uses 2 specificity levels (specific probes and primers) for an accurate
identification of the tested pathogen, avoiding false positive results
• Amplification internal control is included (duplex kits) to avoid false negative results
• Controls included
Analysis throughput
ADIAFOOD® kits suit to your analysis throughput with 2 formats:
> Microplates: ideal for a high number of tests
= up to 88 samples per plate
> Flexiwells: adapt your run to your analysis (numbers of samples
and tested pathogens)
= 8 samples per flexiwells
Several pathogens can
be tested on the same
microplate!
Open platform
The Stratagene thermocycler is an opened platform that can be used with
other PCR kits (ex: Legionella ADIACONTROL® kits)
Automated results interpretation
via Sentinel® software
> Follow-up of each analysis step
> Data management tool
> Analysis certificate delivered per microplate or per sample
> LIMS connexion
Automated results analysis with Sentinel® Software: no curve interpretation!
ADIAFOOD® services
ADIAFOOD ® services
Training
Support
Service
Benefit from a taylor-made PCR solution!
• Training: ADIAGENE and AES CHEMUNEX are registered as official training facilities
• Support in laboratory organisation (flow management, drafting plans...)
• Hotline
Expertise in molecular biology
Legionella
ADIACONTROL®
Food pathogens
ADIAFOOD®
Veterinary pathogens
www.aeschemunex.com
AES ChEmunEx uK
Endeavour house
3rd floor • London
Stansted Airport
Essex • CM24 1SJ
Tel.: +44 (0)1279 666 356
Fax: +44 (0)1279 666 357
[email protected]
AES ChEmunEx Germany
Wilhelmstraße 49
D-74918 Angelbachtal
Tel.: +49(0)7265 9179 10
Fax: +49(0)7265 9179 15
[email protected]
AES ChEmunEx France
(headquarters)
Rue maryse Bastié
Ker Lann CS17219
35172 Bruz cedex
Tel.: +33 (0) 2 2350 1212
Fax: +33 (0) 2 2350 1205
[email protected]
AES ChEmunEx Benelux
Schumacherstr. 12
D-76275 Ettlingen
Tel.: +49 151 1160 4149
Fax: +49 7243 718093
AES ChEmunEx France
Immeuble Paryseine
3, allée de la Seine
F-94854 Ivry-sur-Seine cedex
Tel.: +33(0)1 4959 2000
Fax: +33(o)1 4959 2001
[email protected]
AES ChEmunEx, Inc uSA
1 Corporate Drive
Cranbury nJ 08512
Tel.: +1 609 235 9272
Fax: +1 609 235 9298
[email protected]
www.adiagene.com
AES ChEmunEx Spain
Pol. Ind. Santa margarida II
C/ Albert Einstein, 44
08223 TERRASSA (Barcelona)
Tel.: +34 93 785 36 27
Fax: +34 93 784 72 63
[email protected]
AES ChEmunEx Italy
Via Panà, 56/b
35027 nOVEnTA PADOVAnA
(PD)
Tel.: +39 049 87 05 804
Fax: +39 049 87 09 691
[email protected]
38, rue de Paris
22000 St-Brieuc • France
Tel.: (33) 02 96 68 40 20
Fax: (33) 02 96 68 37 93
[email protected]
© Masson Communication 2008 • DA280A
ADIAVET®