Hellenic Accreditation System Annex F1/A26 to the Certificate No 139-11 SCOPE of ACCREDITATION of the Analytical Laboratory of BIOLAB S.M.P.COMPANY Tested materials/products Types of test/Properties Applied Methods/Techniques Chemical Testing 1. Water Determination of alkalinity Determination in bicarbonate 2. Potable water Determination of Ba 3. Potable water Determination of Se 4.Potable water, groundwater Superficially, waste water Determination of nitrate (in waters with low organic matter) APHA *2320, 21η ΕΚΔΟΣΗ, 2005 XN 33** APHA *2320, 21η ΕΚΔΟΣΗ, 2005 XN 33** In house method based on Standard Methods of examination 3113 Α- B AAS Method XN 15** In house method based in Standard Methods of examination 3111 Α&Β Metals By F AAS XN 25** In house method based on APHA* 4500-NO3: 20thedition, 1998 (Ultraviolet spectrophotometric) XN 08** Determination of nitrite (in non treated waters) In house method based on APHA* 4500-NO2: 20th edition, 1998 (colorimetric – azo dye) XN 06** Determination of ammonia In house method based on APHA* 417 B: 17thedition, 1989 (Nesslerization method) XN 07** Determination of calcium (ions) 3111B, 3500Ca, Α (APHA, Standard Methods 20th Ed. 2000) XN 03** Page 1 of 8 Annex F1/A26 to the Certificate ESYD Nο 139-11 7.6.2017 Tested materials/products 4.Potable water, groundwater Superficially, waste water Types of test/Properties Applied Methods/Techniques Determination of magnesium (ions) In house calculation method, based on 3500-Μg B calculation method (APHA ,Standard Method 21th Ed. 2005) (continued) XN 04 ** Determination of phosphates 4500 P, E (APHA, Standard Methods 21th Ed. 2005) XN 10 ** Determination of hardness 2340 C (APHA, Standard Methods 21th Ed. 2005) Determination of easily liberated and free cyanide Determination of surfactants Determination in turbidity Determination of residual chlorine Determination in Color Determination in oxidisability (KMnO4 consumption) Determination in B (boron) Determination of sulphate-SO4 Determination of Fe (Iron) Determination of Pb, Cd, Ni, As , Co,Sb,Cr ,Ag ,V Determination of Na, K, Zn, ,Cu Determination of Mn,Al Determination of Hg Page 2 of 8 XN 04 ** In house method XN 32** using Merck Spectroquant 1.09701 In house method ΕPΑ425.1, XN 34** In house method HANNA kit test XN 31** In house method EPA DPD METHOD 330.5 XN 30** In house method ISO ΕΛΟΤ 657/1983 with photometer XN 36** ISO ΕΛΟΤ 827/1986 XN 37** ISO ΕΛΟΤ 9390/1990 XN 35* APHA 4500Ε ,21η ΕΚΔΟΣΗ, 2005 XN 28** In house based Merck kit test XN 39** In house method based on Standard Methods of examination 3113 Α- B AAS Method XN 15** In house method based on Standard Methods of examination 3111 Α&Β Metals By F AAS XN 14** In house method based in Standard Methods of examination 3111 Α&Β Metals By F AAS XN 25** In house method based on Standard Methods of examination 3112 B Cold-Vapor AAS Method XN 16** Detaermination of ( SiO ) Merck Kit test based on APHA 4500Si E , DIN 28405 D21 MERCK 1.14794.0001 Κωδικός μεθόδου: XN 44** Determination of TDS In house based on APHA 2540C Κωδικός μεθόδου: XN 38** Annex F1/A26 to the Certificate ESYD Nο 139-11 7.6.2017 Tested materials/products 4.Potable water, groundwater Superficially, waste water Types of test/Properties Electrometric determination of acidity (pH) Applied Methods/Techniques APHA* 4500-H+: 20th edition, 1998 XN 01** Determination of conductivity (continued) APHA* 2510: 20thedition, 1998 XN 02** Determination of BOD5 In house method X/N11 using manometer, based on 5280D (APHA, Standard Methods 21th Ed. 2005) XN 11 Determination of COD ISO 15705: 2002 Determination of (SS) suspended solids Determination of chloride ion-Cl Determination of F Determination of Cr (IV) Determination of sulfide Determination of total, organic and inorganic nitrogen Determination of total organic carbon ( TOC) 5. Sludges Determination of Pb Cd Ni As Cr Determination of Mn Al Determination of Zn Cu Determination of Hg Page 3 of 8 XN 12 In house method X/N 28 based in APHA: 2005, 2540D, 2-58 XN 28 Ιn house based on APHA 4500-Cl B XN 09** In house method based in Standard Methods of examination with kit MERCK , based in EPA340.3 και APHA, Standard Methods 4500 –F E XN 26** APHA: Standards methods for examination of Water and Wastewater, 21th edition 2005. XN 13 ** Merck kit based on EPA 376.2 APHA 4500S2-D, ISO 10530, DIN 38405 D26 MERCK 1.14779.0001 XN39** In house calculative method based on APHA 4500-Norg, APHA-NO3 APHA 4500-NO2 XN40 Merck Kit test MERCK 1.14878.0001, XN41** In house method using AAS Κωδικός μεθόδου: XN 15** In house method using F AAS Κωδικός μεθόδου: XN 25** In house method using F AAS και emission spectometry Κωδικός μεθόδου: XT 28 In house method using Cold-Vapor AAS Κωδικός μεθόδου: XN 16** Annex F1/A26 to the Certificate ESYD Nο 139-11 7.6.2017 Tested materials/products Types of test/Properties 6.Fish and fishery products , cereals and feed 7.Foods , cereals and feed Determination of Hg 8.Foods and feed (except diary) 9.Cereals and nuts Determination of Cd Applied Methods/Techniques In house method by cold vapor AAS XY 29 Determination of Pb In house method based in AOAC official method 999.10 XY 22 Determination of As 10.Cereals, vegetables and feeds Determination of K In house method by F AAS spectroscopy XT 28 11.Cereals and feeds Determination of 12. Cereals and feeds Determination of Na 13. Cereals and feeds 1. Determination of Moisture Fe Mn Zn Ca In house method by F AAS spectroscopy XT 28 Directive 71/ 393 ΕΕC XT 02 2. Determination of Ash Directive 71/ 393 ΕΕC 3. Determination of Fat Content ΧΤ 01 Directive 71/ 393 ΕΕC 4. Determination of Protein 14.Cheese In house method by F AAS spectroscopy XT 28 ΧΤ 03 In house method Χ/Τ/04, modified based on ΑOAC 991.20:2000 1. Determination of Moisture Food Code, Volume ΙΙ, Part B ,Θ3.Ι and Γ1 ΧΤ 10 2. Determination of Fat Content In house method Χ/Τ 11 , Gerber method 15. Milk 1. Determination of Protein 2 Determination of fat free dry total matter In house method Χ/Τ/04, modified based on ΑOAC 991.20:2000 Food Code, Volume ΙΙ, Part B, Θ5 ΧΤ 08 3 Determination of Fat Content In house method Χ/Τ09 (Gerber method) 16. Meet and meet products 1. Humidity ΧΤ 09 ISO 1442:1997 ΧΤ 06 2. Ash ISO 936 :1998 ΧΤ 05 3.Total fat Food Code, Volume II, Part B ,Ι3 and Γ4 ΧΤ 07 In house method Χ/Τ/04, modified based on ΑOAC 991.20:2000 4. Protein 17.Fruits and vegetables Page 4 of 8 Protein In house method Χ/Τ/04, modified based on ΑOAC 991.20:2000 Annex F1/A26 to the Certificate ESYD Nο 139-11 7.6.2017 Tested materials/products Types of test/Properties Applied Methods/Techniques In house method Χ/Τ/04, modified based on ΑOAC 991.20:2000 18. Legumes Protein 19. Pre-packed Foods and non prepacked foods 20. Milk Determination of total sulfur (SO2) Volumetric method based K.T.P. To the Section D paragraph D2 ΧΤ 30 Determination of freezing point, In house method based in ISO 5764/2009 using thermistor cryoscope method thermistor cryoscope Determination of antibiotic residues in milk, DELVOTEST KIT X/Τ 38 In house method based in DELVOTEST KIT X/Τ 41 21.Milk and milkbased liquid products Determination of Total Fat Total Protein Lactose Total Solids Internal methodΕσωτερική μέθοδος βασισμένη based in infrared phasmatophotometer (CRYOSTAR ANALYSER) XT 39 22.Oils 1. Determination of peroxide value Regulation EEC 2568/91 (Αnnex II) ΧΤ 13 Food Code, Volume ΙΙ, Part B ,Θ3.Ι and Γ1 ΧΤ 10 2. Determination of Moisture 23.Honey 3. Determination of K232 -K270 Regulation EEC 2568/91 (Αnnex II) ΧΤ 15 4. Determination of acidity (acid value) Regulation EEC 2568/91 (Αnnex II) ΧΤ 14 HMF Determination (HydroxyMethylFurfural) Internal method: UV-VIS XT-21 Diastade determination based on Internal method UV-VIS Schade method XI-54 (diastase index, diastase activity) 24. Beers,Wine, Alcoholic beverages up to 99% vol 25. Soil Determination of Alcoholic strength Determination of Ca Mg K Structural Composition Sand % Slurry % Clay % pH Page 5 of 8 Automated alcoholic distiller . XT 42 In house based ΧΤ 33 ,based on Plant, Soil and Water Reference, Methods for the Western Region.1994 WCC-103 Publication WREP-125, 2 ndEdition , abd AAS Flame Internal method ΧΤ 36 , Based on Plant, Soil and Water Reference, Methods for the Western Region.1994, WCC S14-10 , WCC-103 Publication WREP-125, 2 ndEdition Internal method ΧΤ 18 Based on WCC-103 PublicationWREP-125, 2nd Edition Annex F1/A26 to the Certificate ESYD Nο 139-11 7.6.2017 Tested materials/products Types of test/Properties Conductivity Organic Substance Determination of Total Phosporus (OLSEN) Determination of Nitric Nitrogen (NO3 -1 ) Applied Methods/Techniques Internal method ΧΤ 19 Based on WCC-103 Publication WREP-125, 2 ndEdition Internal method ΧΤ 20 (Walkley – Black, titrimetric) WCC-103 PublicationWREP-125, 2 ndEdition Internal MEthod ΧΤ 28 (OLSEN) Based on Plant, Soil and Water Reference, Methods for the Western Region.1994, WCC-103 Publication WREP-125, 2 ndEdition Internal method ΧΤ 35 , Based on Plant, Soil and Water Reference, Methods for the Western Region.1994, WCC-103 Publication WREP-125, 2 ndEdition Microbiological Testing 1.Water: Potable, salt, surface, swimming pool Detection and enumeration of intestinal enterococci ΕΝ ISO 7899-2000 M-03* Detection and enumeration of E.Coli and Coliforms ΕΛΟΤ ΕΝ ISO 9308-1:2014 M-01* 2.Water: Potable, Swimming pools Detection and enumeration of Clostridium perfringens ISO 14189:2013 Enumeration of culturable microorganisms in 22 οC and 37 ο C ΕΝ ISO 6222:1999 Detection and enmeration of Pseudomonas aeruginosa ISO 16266:2006 Μ-09 M -02* M-05* 3. Food and feedstuffs Enumeration of Total Aerobic Count at 30oC ΕΝ ISO 4833-1:2013 MT -02 Detection and Enumeration of Coliforms ΕΝ ISO 4832:2006 MT-01 Page 6 of 8 Annex F1/A26 to the Certificate ESYD Nο 139-11 7.6.2017 Tested materials/products Types of test/Properties Detection and enumeration of E. coli β-glycoronidase (+) Applied Methods/Techniques ΕΝ ISO 16649-2:2001 MT-12 Detection and enumeration of Staphylococcus coagulase (+) ISO 6888-2/A1:1999 MT-04 Detection of Salmonella spp ΕΛΟΤ ΕΝ ISO 6579:2002/Corr 1 MT-05 Detection of Listeria monocytogenes ΕΝ ISO 11290-1/A1:2004 Amd 1:2004 MT-06 Detection and enumeration of Clostridium perfringens ΕΝ ISO 7937:2004 Detection and enumeration of Enterobacteriaceae MT-08 ISO 21528-2: 2004 MT-13 Detection and enumeration of Bacillus cereus ISO 7932:2004 ΜΤ-19 Detection and enumeration of lactic acid bacteria 30 oC ΙSΟ 15214 / 1998 4. Food and feedstuffs with aw >0.95 Detection and enumeration of yeasts and moulds ISO 21527-1:2008 5. Food and feedstuffs with aw<0.95 Detection and enumeration of yeasts and moulds Κωδικός μεθόδου: MT-22 MT-03 ISO 21527-2:2008 ΜΤ-21 Sampling 1. Surface sampling using the swab technique Horizontal method for microbiology tests ISO 18593:2004 ( Object surfaces) Potable water from water supply and water drills ISO 5667-1:2006 ISO 5667-3:2006 ISO 19458 : 2006 Sea water and environmental samples ISO 5667-9:2006 ISO 5667-14:2006 ISO 19458 : 2006 Page 7 of 8 Sampling of water for chemical Annex F1/A26 to the Certificate ESYD Nο 139-11 7.6.2017 Tested materials/products Types of test/Properties testing Sludge sampling Waste water sampling Applied Methods/Techniques ISO 5667-13:2011 ISO 5667-15:2011 ISO 19458 : 2006 ISO 5667:1992 ISO 19458 : 2006 * American Public Health Association, American Water Works Association, Water Environment Federation, “Standard Methods for the Examination of Water and Wastewater”, 20 th Edition, 1998 and 21st Edition 2005 (**) Methods highlighted with (**) have been assessed and conform to the performance criteria described in ΚΥΑ Υ2/2600/2001 , ΦΕΚ 892/Β/11.07.2001, its modification ΚΥΑ Αριθμ. ΔΥΓ2 / Γ.Π οικ.38295 regarding the quality of drinking water. Site of assessement : Permanent Laboratory premises, , Arta, Greece Approved signatory : Sotiris Tzimas , Fotios Lampros , Lenis Gregory This scope of Accreditation replaces the previous one dated 23.2.2017 The Accreditation Certificate No.139-11, to ELOT EN ISO/IEC 17025:2005, is valid until 15.9.2019. Athens7.6.2017 Ioannis Sitaras Director of the Laboratories Accreditation Division Page 8 of 8 Annex F1/A26 to the Certificate ESYD Nο 139-11 7.6.2017
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