Name: ______________________________________ Per. _____ Date: ___________________ Lab partner’s name: ____________________________________ Background: Dr. Nefario needs to help the Minion species stay genetically varied to help protect them from extinction (cuz let’s face it, the world would be a sad place without minions). Since all Minions are male, natural reproduction is not an option, but Dr. Nefario doesn’t want to simply clone Minions. Instead, he is going to take one chromosome from each Minion and combine it with a second chromosome from another Minion. Minions only have one pair of chromosomes and all the genes are on those chromosomes. Therefore, each genetic trait has two alleles. Today, you are going to assist Dr. Nefario with making more Minions and increasing Minion genetic diversity. Learning Target: Make a Minion Genetics I Can… Demonstrate my understanding of Mendelian genetics and Punnett square probability predictions. I know I am successful when I can… Determine genotypes and phenotypes for Dominant/Recessive alleles. Predict potential offspring genotype ratio and phenotype percentage for monohybrid crosses of traits. Complete a dihybrid cross for two traits. Create a Minion by translating genotype into phenotype for various traits. Procedure: 1. Use a coin to determine what alleles are present on chromosome number 1 and chromosome number 2 for each trait. Heads = Dominant Tails = Recessive a. Refer to the Trait Chart below to determine which allele version is dominant and which allele version is recessive. b. Record your allele results for each trait for chromosome number 1 under the appropriate column in Data Table 1. c. Repeat the coin flip procedure and record allele results for chromosome 2 under the appropriate column in Data Table 1. 2. Record the genotype for each trait under the appropriate column in Data Table 1. 3. Determine the phenotype for each trait from the genotype and record in the appropriate column of Data Table 1. 4. Once your minion is finished, pair up with a different group and determine what an offspring Minion would look like if their genetics were crossed. a. Complete Data Table 2 with the information about the other group’s Minion. (make sure you get this info before going any further) b. Complete the Punnett squares for each cross. c. Determine the genotype ratio for each cross. d. Determine the phenotype percentage for each cross. 5. Complete a dihybrid cross between your Minion and the other Minion for two traits. 6. Use the phenotype for each trait from Data Table 1 (Your Minion) to create your minion. a. Use the template material packet and markers/crayons to give your Minion the appropriate physical features determined by their genetic makeup. Minion Trait Chart Trait Body color Height Body shape Number of eyes Color of eyes Hands Hair Personality Dominant Allele Yellow (Y) Tall (T) Stocky (S) Two (E) Brown (B) 3 fingers (F) Spikey hair (H)* *If Minion is purple and has spikey hair, then the hair is a big straight-up tuft. Outgoing (O) [open smile] Recessive Allele Purple (y) Short (t) Skinny (s) One (e) Green (b) 4 fingers (f) Flat hair (h)* *Purple minions have fluffy flat hair. Shy (o) [closed smile] Data Table 1 (Your Minion) Trait Chromosome 1 Allele Chromosome 2 Allele Genotype Phenotype Chromosome 2 Allele Genotype Phenotype Body color Height Body shape Number of eyes Color of eyes Hands Hair Personality Data Table 2 (Other Group’s Minion) Trait Body color Height Body shape Number of eyes Color of eyes Hands Hair Personality Chromosome 1 Allele Analysis: Using the genotype of your Minion and another group’s Minion, complete each Punnett square, genotype ratio, and phenotype percentage for each designated trait. 1. Cross your Minion and the other group’s Minion for the trait of body color. a. Complete the Punnett square for the monohybrid cross. b. What is the genotype ratio for the possible offspring? c. What is the percentage chance of having a purple offspring? 2. Cross your Minion with the other group’s Minion for the trait of number of eyes. a. Complete the Punnett square for the monohybrid cross. b. What is the genotype ratio for the potential offspring? c. What is the percentage chance of having an offspring with 2 eyes? 3. Complete a Punnett square for a dihybrid cross for both the traits of height and body shape. 4. What is the percentage chance of having an offspring Minion that is both tall and skinny? 5. What is the percentage chance of having an offspring Minion that is both tall and stocky? 6. What is the percentage chance of having an offspring Minion that is both short and stocky? 7. What is the percentage chance of having an offspring Minion that is both short and skinny?
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