Supplementary Table 2. Top ~40 peptides from the each round of 4G2 affinity
selection using a mutagenic library based on the peptide shown in the shaded row.
Fold enrichment of peptides was calculated by dividing % total of the current round by
the previous round % total. Coloring allows comparison of enrichment across the
selection, and underlined peptides were further tested for immunogenicity.
Original
mutagenic
library
# of
reads
%
of
total
Round 1
peptide
selectants
# of
reads
%
of
total
Fold
enrich
-ment
Round 2
peptide
selectants
# of
reads
% of
total
Fold
enrichment
NWDPTQFPGK
99
0.105
NWDPTQFPGK
1511
2.168
20.66
NWDPTQFPGK
12115
8.180
77.98
KWDPTQFPGK
NWDPTQLPGK
NRDPTQFPGK
NWDPTQFPGN
NWEPTQFPGK
NLDPTQFPGK
NWDPTKFPGK
NWDTTQFPGK
NCDPTQFPGK
NWDPSQFPGK
NWDPTQFPRK
NWDPTQFSGK
NWDPTQIPGK
NWNPTQFPGK
NGDPTQFPGK
NWDPTHFPGK
NWDPTQFPGT
NWDPTQVPGK
NWDLTQFPGK
NWDPNQFPGK
YWDPTQFPGK
NWDPTQSPGK
NWDPTQYPGK
SWDPTQFPGK
NSDPTQFPGK
NWDPIQFPGK
NWDPTQFPDK
TWDPTQFPGK
IWDPTQFPGK
NWDPTLFPGK
NWDPTQFPCK
NWDRTQFPGK
NWDSTQFPGK
NWHPTQFPGK
HWDPTQFPGK
NWAPTQFPGK
NWDPTQFAGK
NWDPTQFLGK
…
NWDPTRFPGK
NWDPIKFPGK
NWDPAKFPGK
34
32
30
25
25
24
20
19
18
18
18
18
18
18
17
17
17
17
16
16
16
15
15
15
14
14
14
14
13
13
13
13
13
13
12
12
11
11
0.036
0.034
0.032
0.026
0.026
0.025
0.021
0.020
0.019
0.019
0.019
0.019
0.019
0.019
0.018
0.018
0.018
0.018
0.017
0.017
0.017
0.016
0.016
0.016
0.015
0.015
0.015
0.015
0.014
0.014
0.014
0.014
0.014
0.014
0.013
0.013
0.012
0.012
890
766
681
664
605
441
395
392
369
368
361
355
339
335
332
324
321
319
319
314
314
302
295
292
283
281
270
259
257
249
247
247
243
223
223
220
220
220
1.277
1.099
0.977
0.953
0.868
0.633
0.567
0.562
0.529
0.528
0.518
0.509
0.486
0.481
0.476
0.465
0.460
0.458
0.458
0.450
0.450
0.433
0.423
0.419
0.406
0.403
0.387
0.372
0.369
0.357
0.354
0.354
0.349
0.320
0.320
0.316
0.316
0.316
86.06
69.13
NWDPIQFPGK
NWDPIKFPGK
NWDPTKFPGK
NWDPNQFPGK
NWDPAKFPGK
NWDPTQYPGK
NWDPVKFPGK
NWDPTRFPGK
NWDPNKYPGK
NWDPAQFPGK
NWDPTKFPGM
NWDPSQFPGK
NWDPARYPGK
NWDPTQYPGM
NWDPAKYPGK
NWDPTRYPGK
NWDPKQFPGK
NWDPIQFPGM
NWDPSKFPGK
NWDPTQFPGM
NWDPIQFPGR
NWDPIQYPGK
NWDPTQYPGL
NWDPVQFPGK
NWDPNQFPGM
NWDPNQYPGK
NWDPTKYPGK
NWNPTQFPGK
NWDPSEFPGK
NWDPTQFPGQ
NWDPSQYPGQ
NWDPIKFPGY
NWDPDRFPGK
NWDPCQFPGK
NWDQNKFPGM
NWDPYKFPGK
NWDPRRFPGK
GWDPLKFPGK
…
12049
7310
6599
5653
5505
5340
4284
3684
3266
2962
2286
1988
1760
1641
1634
1602
1537
1399
1393
1235
1107
1082
1028
1013
997
954
914
880
870
751
632
622
604
584
584
541
515
502
8.136
4.936
4.456
3.817
3.717
3.606
2.893
2.487
2.205
2.000
1.544
1.342
1.188
1.108
1.103
1.082
1.038
0.945
0.941
0.834
0.747
0.731
0.694
0.684
0.673
0.644
0.617
0.594
0.587
0.507
0.427
0.420
0.408
0.394
0.394
0.365
0.348
0.339
548.39
132.34
210.24
4.01
5.88
3.28
11
1
1
0.012
0.001
0.001
NWDPIQFPGK
NWDPTQYPGK
NWDPTQFPDK
NWDPNQFPGK
NWDPTKFPGK
NWDPAKFPGK
KWGQTALPDM
KWDATWFPVN
NWNPTQFPGK
NWDPTQFPGY
NWDSTQFCGN
NWDPTRFPGK
NWKPIQLPGT
SQDPTQYSDK
NSVPSQYSGK
NWGPNKFPGK
TWHPSQFHGM
IYYQTTFSGY
NWDPTQYPGL
NWDPAQFPGK
NWDPIQFRGK
NWDPSQFPGK
IGDPRQCSVQ
NWNPTLYQYM
HWADTQFHRT
NWDQNKFPGM
NWDPTQCTTN
HLDHTDLPSK
NWDTTQFPDK
NWDPTDIPGK
NCVPIQLFRK
TWDPPQVPGK
RGDQTQFLGK
NGNPTQVPCE
NRVPTKIHDR
NWDPTEYPCK
NWDPTQFPGM
TSDSTQCAGK
…
NWDPIKFPGK
26
0.037
35.20
56.18
40.95
597.0
27.75
43.69
4.88