Microbiology –Tentative Schedule
Fall 2016
o Page # in ( ) denote pages for 3rd edition of Lecture Textbook
o For Lab Exercises the page numbers are for the 3rd edition of the Lab Manual
This is a guide and is subject to change at the discretion of the instructor. You will be informed of any changes
Date
Lecture Topic
Lecture
Lab Schedule
Lab Exercise
Reading
8/30
Tues
Lab 1
Syllabus, Modern
Age of
Microbiology,
Chap 1
o
o
o
o
o
9/1
Thurs
Lab 2
Cell Structure and
Function
Chap 3
o
o
o
o
o
9/6
Tues
Lab 3
Cell Structure and
Function
Chap 3
o
o
o
o
o
9/8
Thurs
Lab 4
Classification and
ID of
Microorganisms
Chap 4
o
o
o
o
o
9/13
Tues
Lab 5
Microbial Nutrition
and Growth
Last day to get
presentation
topic approved
Chap. 6 pp.
182 – 190
(pp. 183-195)
(rest of chap
covered in
lab)
o
o
o
o
9/15
Thurs
Lab 6
Microbial Nutrition
and Growth
Chap. 6
o
o
o
o
o
o
o
9/20
Tues
Lab 7
MDufilho
Controlling
Microbial Growth
in the Environment
Chap. 9
o
o
Page 1
Lab 1: Safety & Intro to Media
Safety & Lab Practices pp. 1-13
Lecture text Chap. 6 pp. 167-170
&177-181 (167-170 & 178 – 182)
Media Prep - Demo
Ubiquity of Bacteria
o
o
Lab 2: Tools of the Lab
Results
Lecture text Chap. 6 pp.174 – 177
(pp.175-178)
Aseptic Technique – read p.31
Streak Plate
o
Lab Quiz 1 – 25 pts (pp. 1-6, 1.3,
2.1 & notes)
Lab 3: Colony Morphology &
Growth in Broth
Results
Colony morph.
Growth in broth
o
Lab 4: Microscopy
Lecture text Chap. 4 pp. 96-101
(pp.97-102)
Results
Microscope Care and use
Review standard
shapes/arrangements of bacteria –
Lecture text Chap. 11 322 - 325
o
o
o
3.1 p. 142
Read p. 141
Examine prepared slides
Lab Quiz 2 – 25 pts (1.4, 1.5, 2.2,
2.3. 2.4 & notes)
Lab 5: Specimen Prep, Staining &
Aerotolerance
Lecture text Chap.4 pp. 106 – 111
(pp.107 - 112)
Aerotolerance Chap. 6 pp.166-168
(pp.168-169)
o
2.6 p. 95 Fluid
Thioglycollate Medium
2.11 p.125 Steam
Sterilization
Lab 6: Simple & Negative Stain
Results
Read and use pp. 153 - 158
Chap. 11 pp.322 – 325 (pp. 320322)
Simple stain
Negative stain
Aseptic technique test – 25pt
o
o
3.4 p. 177 Simple Stain
3.5 p. 183 Negative Stain
Lab Quiz 3 – 25 pts. (3.1, 2.6, 2.11
& notes)
Lecture text Chap. 3 cell walls pp.
o
3.6 p. 187 Gram Stain
o
o
o
o
o
1.3 Demo p. 25
2.1 p.61 Ubiquity of
Microorganism
Read p. 59-60
1.4 p. 31 Aseptic Transfers
& Inoculation Methods
1.5 p. 45 Streak Plate
Method of Isolation
2.2 p. 67 Colony
Morphology
2.3 p. 79 Growth Patterns
on Slants
2.4 p. 83 Growth Patterns
in Broth
9/29/2016
9/22
Thurs
Exam #1 (Ch.
1,3,4,6) – 100
points
9/27
Tues
Lab 8
Controlling
Microbial Growth
in the Environment
Chap. 9
9/29
Thurs
Lab 9
Microbial
Genetics
Chap. 7
mastering
Micro Special
Assignment
Chap. 7
10/4
Tues
Lab 10
Microbial Genetics
o Due – Chap.
7 mastering
Micro Special
Assignment –
25 points
Chap. 7
10/6
Thurs
Lab 11
Recombinant DNA
Chap. 8
10/11
Tues
Lab 12
Recombinant DNA
o Group
Assignment
for Ethics
Study
10/13
Thurs
Lab 13
Library
Assignment –
Required
NO LECTURE
10/18
Tues
Lab 14
Controlling
Microbial Growth
in the Body
10/20
Thurs
Lab 15
Exam 2 – 100 pts.
(7, 8, 9)

o
63 – 66 (pp. 65 – 68)
Gram stain
o
o
Acid Fast Stain
Capsule stain
o
Lab Quiz 4 – 25 pts. (3.4, 3.5, 3.6
& notes)
Endospore stain
Motility
Flagella – review prepared slides
o
o
3.7 p. 195
3.8 p. 201
o
o
o
o
3.9 p. 205
3.10 p. 211
3.11 p. 217
Morphological Unknown – 25
points
Turn in Data Sheet for 3.12
o
3.12 p. 221
Lecture text Chap. 10, pp. 298 -300
(pp. 297 – 299)
Antibiotic Sensitivity Test
o
o
7.2 p. 447
Read pp. 441 - 442
o
o
Lab Quiz 5 – 25 pts. (3.7, 3.8 3.9,
3.10, 3.11, & notes)
Selective/differential media
Results antibiotic sensitivity test
o
o
o
4.1 – 4.6 p. 229
5.21 p. 385
5.23 p. 395
Read p. 227 -228
o
o
Quiz 6 – 25 pts ( 7.2 and notes)
Results
Lab Quiz 7 – 25 pts. (4.1 – 4.6,
5.21, 5.23 and notes)
Differential Tests - Oxidation and
Fermentation media and Gelatin
hydrolysis
o
o
o
5.1 – 5.6 p. 271
5.14 p. 349
Read 267 - 270
Results
Lecture Chap. 12 – Introduction of
Eukaryotes pp. 353 – 357, 358, 360
– 370
Microscopic Observation of
Eukaryotes
o
3.3 – Eukaryotic
Organisms p. 159
Observe slides of various
eukaryotes
Lab Quiz 8 – 25 pts. (5.1 – 5.6,
5.14, and notes)
o
o
o
o
o
o
o
o
Chap. 8
o
NO LAB
Chap. 10
o
o
o
o
o
10/25
Tues
MDufilho
Characterizing
and Classifying
Chapter 11
and
o
Page 2
o
5.7 – 5.10, 5.12, 5.13,
5.15, 5.16, 5.18. 5.19 p.
9/29/2016
Lab 16
o
Prokaryotes
Turn in
Research
Paper– 75
points
selections
from Chap.
19-24
10/27
Thurs
Lab 17
Characterizing
and Classifying
Prokaryotes
Group Reports
on Ethics Project
Chapter 11
and
selections
from Chap.
19-24
o
11/1
Tues
Lab 18
Characterizing
and Classifying
Prokaryotes
(Turn in pres.
draft - 25 pts.)
Chapter 11
and
selections
from Chap.
19-24
o
o
o
Quiz 10 – 25 pts (5.7 – 5.10, 5.12,
5.13, 5.15, 5.16, 5.18. 5.19 and
notes)
Unknown Chart Due – 25 points
Discussion on Unknown Project
Characterizing
and Classifying
Eukaryotes
Chapter 12
and
selections
from Chap.
19-24
o
Unknown Project
o
11/3
Thurs
Lab 19
11/4
o
o
Utilization, Decarboxylation,
Deamination and Hydrolytic
Enzymes
Combination Differential media
313
Quiz 9 – 25 pts. (3.3 and notes on
Eukaryotic organisms)
Results
Last Day to
Withdraw
11/8
Tues
Lab 20
Characterizing
and Classifying
Eukaryotes
Chapter 12
and
selections
from Chap.
19-24
o
Unknown Project
11/10
Thurs
Lab 21
Classifying
Viruses, Viroids
and Prions
o
Chapter 13
and
selections
from Chap.
19-24
o
Unknown Project
o
o
Unknown Project
o
o
Unknown Project Due (75 points)
at beginning of class.
Instructions on Morbidity & Mortality
Assign Morbidity & Mortality
Report
o
11/15
Tues
Lab 22
11/17
Thurs
Lab 23
11/22 –
24
11/29
Tues
Lab 24
Exam 3 – 100
points (10-11 &
Chap 19 - 24)
Classifying
Viruses, Viroids
and Prions
Chapter 13
and
selections
from Chap.
19-24
Thanksgiving
Holiday
No Lecture
Classifying
Viruses, Viroids
and Prions
o
o
No Labs this Week
Chapter 13
and
selections
from Chap.
19-24
o
o
o
o
MDufilho
7.3 p. 455
Page 3
Morbidity & Mortality Report Due
– 25 points
Lecture text Chap 13, pp. 390 – 394
Lecture text Chap. 13 pp. 405 – 406
(pp.400-402)
Culturing Viruses
9/29/2016
12/1
Thurs
Lab 25
Exam 4- 100
points
(Ch,12 -13, Chap
19 – 24))
o
Plaque Assay
12/6
Tues
Lab 26
Class
Presentations –
75 pts.
o
Results and Discussion of Plaque
Assay
Quiz 11 – 25 pts. (6.4 & notes)
Lab Clean Up – all must be in
attendance
12/8
Thurs
Class
Presentations –
75 pts.
12/15
Thurs
Mandatory Final
Comprehensive
10:30 – 12:30
12/16
MDufilho
o
o
o
6.4 p. 425
No Lab
No Lab
Finals Week
Grades Due
Page 4
9/29/2016