TRIglyphix Sense
®
A Natural Active for Sensitive Skin
AT A GLANCE
Sensitive skin is a hyper-reactive condition affecting millions of people worldwide. It is typically perceived by
consumers as a reaction to cosmetics and other topically-applied preparations that can generate uncomfortable
skin sensations. Inflammation is another characteristic that is frequently associated with sensitivity and can be
perpetuated by factors such as UV exposure, lifestyle, stress or hormonal changes.
TRIglyphix® Sense is a botanically-based active designed to quell the discomfort and redness associated with skin
irritation. This novel active is derived from renewable resources and especially suited for people with sensitive skin.
Based on DNA microarray analysis, in vitro and in vivo studies, results show TRIglyphix® Sense delivers superior
soothing properties, while enhancing the expression of genes involved in barrier function, stress response and
repair mechanisms.
RESEARCH METHODS:
• Genomic research - DNA microarray
• In vitro efficacy testing
• Consumer in vivo studies
INCI:
Hordeum Vulgare Seed Extract
RECOMMENDED USAGE LEVEL:
2-5%
PRODUCT FEATURES
KEY BENEFITS
SUGGESTED APPLICATIONS
• Botanically derived
• Effectively soothes &
protects skin
Sensitive skin products
• Facial moisturizers,
serums & creams
• Cleansers
• Hand & body lotions
• Made of renewable resources
• Gluten-free
• Non-GMO
• Paraben-free
• BSE-free
• Reduces expression of
pro-inflammatory biomarkers
• Shown to reduce redness,
burning & stinging
• Delivers anti-aging benefits for sensitive skin formulations
Shaving applications
• Shave creams & gels
• After-shave applications
Anti-aging products
• Facial moisturizers,
serums & creams
THE COMPLETE ACTIVE
FOR SENSITIVE SKIN
Soothing
Anti-Inflammaging
Addresses the neuro-sensory aspect (burning & stinging)
associated with sensitive skin
Shown to help reduce pro-inflammatory biomarkers
Shown to help reduce discomfort associated with irritating anti-aging actives
Shown to minimize the appearance of redness associated with irritation
Can help to prevent acute inflammation from becoming a chronic issue
in vitro testing indicates anti-aging benefits
Revitalization
May help rebuild vital components
required for proper healing
Shown to increase extracellular matrix
(ECM) components such as collagen,
hyaluronic acid & elastin
Shown to reduce synthesis of a
destructive enzyme that targets ECM
components (MMP-1)
DNA Microarray Summary TRIglyphix® Sense was shown to modulate the expression of genes necessary for soothing, revitalizing and
repairing sensitive skin. This includes genes involved in the inflammatory cascade, stress response, repair
mechanisms and barrier function.
Keratinocytes
% Difference
(up to)
Fibroblasts
% Difference
(up to)
Repair Mechanisms
66
Protein Formation
77
Stress Response
64
Stress Response
52
Barrier Function
61
Barrier Function
45
Structural Components
67
Skin Development
49
Inflammatory Cascade
-49
Gene Expression Data
A gene is considered to be down-regulated if the ratio of medians is less than 0.7. In a DNA microarray study
testing normal human epidermal keratinocytes, 0.1% TRIglyphix® Sense was shown to down-regulate the
expression of IL-1β and IL-1RAcP. As a result, this data indicates TRIglyphix® Sense may help decrease the
production of pro-inflammatory cytokines while also reducing the inflammatory response generated through
the Interleukin 1 receptor.
Ratio of Medians
Gene Name
Gene Function
0.600
IL1B
Interleukin-1 beta
Inflammatory Cytokine
0.323
IL1RAP
Accessory Protein Interleukin-1 receptor
(IL-1RAcP)
Receptor Accessory Protein
UNTREATED
SOOTHING
IL-1
IL-1
IL-1R1
IL-1RAcP
Cell Membrane
Inflammatory Cascade
Certain cosmetic ingredients, including some surfactants and actives, can irritate the skin and
trigger an inflammatory
MYD88
response. Skin cells such as keratinocytes synthesize and store cytokines, which are released in response to irritation.
IRAK exhibit
Tollip strong proThese include TNFα, IL-1α, IL-1β, IL-6, IL-8 and IL-1RA. The Interleukin-1 (IL-1) family of cytokines
inflammatory effects and are potent mediators of fever, pain and inflammation. IL-1 binds to thePInterleukin receptor to
2+
flux the
trigger the inflammatory cascade. Adjacent to the IL-1 receptor is an accessory protein,TRAF-6
which is needed toCaactivate
®
inflammatory cascade. Previously mentioned DNA microarray data suggests TRIglyphix Sense reduces the expression
of the accessory protein to limit activation through the IL-1 receptor.
NF-kB
MAPK
Expression of
Inflammatory Cytokines
AA
Synthesis of AP-1
dependent proteins
(e.g. MMPs)
COX
PGH2
PGES
UNTREATED
UNTREATED
TREATED
PGE2
TREATED
IL-1
IL-1
IL-1R1
IL-1
IL-1RAcP
IL-1R1
IL-1
Cell Membrane
Cell Membrane
MYD88
IRAK
P
TRAF-6
NF-kB
Expression of
Inflammatory Cytokines
AA
No Signal
Tollip
2+
Ca flux
MAPK
Synthesis of AP-1
dependent proteins
(e.g. MMPs)
COX
PGH2
PGES
PGE2
TREATED
IL-1
Pro-Inflammatory Biomarkers
In the study below, MatTek full thickness tissue models were used to measure a reduction in IL-1α and PGE2. 1%
Sodium Dodecyl Sulfate (SDS) was used to trigger the inflammatory response. TRIglyphix® Sense was shown to
reduce the expression of IL-α and PGE2 by as much as 41% and 49%, respectively.* It was also shown to improve cell
viability compared to cells treated with SDS alone.
Changes in PGE2 Expression
Changes in IL-1α Expression
300
306
Concentration (pg/ml) Endpoint
Concentration (pg/ml) Endpoint
2500
41%
250
34%
200
201
150
182
100
50
2000
2,105
49%
1,643
1500
1000
22%
1,073
500
0
0
1% SDS
1% SDS &
1% TRIglyphix Sense
1% SDS &
5% TRIglyphix Sense
1% SDS
Percent (%) Improvement
1% SDS &
1% TRIglyphix Sense
1% SDS &
5% TRIglyphix Sense
Percent (%) Improvement
Figure 1
Figure 2
*Results are statistically significant (p< 0.05)
56.3
53.4
56.3
13.1
13.1
0.0017% Ascorbic Acid
0.001% TRIglyphix Sense
53.4
0.0017%
0.01% TRIglyphix
Sense Ascorbic Acid
0.001% TRIglyphix Sense
0.01% TRIglyphix Sense
VISIBLE EFFECTS
Cream (placebo)
Baseline
Cream with 5% TRIglyphix® Sense
Day 3
Day 7
12.08%
Redness Reduction
17.84%
Redness Reduction
Figure 3
Baseline
Day 3
44.65%
Redness Reduction
Day 7
89.91%
Redness Reduction
Figure 4
CLINICAL Study 1
Test Material
Redness Reduction
Objective:
To evaluate the performance of TRIglyphix® Sense to reduce redness
and irritation from shaving over a period of seven days.
Study:
• 15 male panelists, ages 22-67
Average % Improvement
via Clinician Rating
Day 3
Day 7
Cream
(placebo)
3.23
12.90
Cream with 2% TRIglyphix® Sense
20.69
44.83
Cream with 5% TRIglyphix® Sense
44.12
70.59
• Test vehicles:
o Group 1: Cream (placebo)
o Group 2: Cream with 2% TRIglyphix® Sense
o Group 3: Cream with 5% TRIglyphix Sense
®
Panelists with similar average irritation were divided into three
groups of five people. Panelists were to shave every day for seven
days and immediately apply the treatment to the shaved area.
Observations were made at Day 0 (baseline), Day 3 and Day 7. A
clinician then evaluated the severity of folliculitis (0-10 scale, best
to worst). In addition, photogrammetrix grading analysis software
was used on a panelist from each group to show changes in the
three dimensional profile of hue, value and chroma.
Results:
TRIglyphix® Sense proved to be soothing and effective for all
users involved in the study. The clinician’s results showed an
average decrease in redness by as much as 70.59% when using
TRIglyphix® Sense. Photogrammetrix results also showed
an average decrease in redness by as much 89.91%. The two
panelists, (Figure 3 & 4), illustrate the clinician’s findings that using
TRIglyphix® Sense will help to visibly reduce redness and razor
burn. This supports the use of TRIglyphix® Sense as an effective
soothing ingredient for sensitive skin.
Burning & Stinging Relief from an AHA
Reduced Sting Intensity in a Gentle Formula
42%
Score
Score
12%
59%
73%
2.5 Minutes
Facial Cream (placebo)
5.0 Minutes
2.5 Minutes
Facial Cream with 2% TRIglyphix Sense
Facial Cream (placebo)
5.0 Minutes
Facial Cream with 2% TRIglyphix Sense
Figure 5
Figure 6
CLINICAL Study 2
CLINICAL Study 3
Burning & Stinging Relief from an AHA
Reduced Sting Intensity in a Gentle Formula
Objective:
Objective:
Study:
Study:
• Test vehicles:
• Test vehicles:
To measure the soothing benefits TRIglyphix® Sense can deliver
by reducing sting discomfort following application of lactic acid.
• 10 panelists (2 male, 8 female) ages 21-50
o Facial Cream (placebo)
o Facial Cream with 2% TRIglyphix Sense
®
A clinician applied 10% lactic acid (aqueous solution) to both
sides of panelists face (nasolabial fold and upper cheek areas) to
induce a feeling of discomfort. After five minutes, the clinician
then applied the placebo to one side of the face and the placebo
with 2% TRIglyphix® Sense to the other side in a blinded fashion.
Following 2.5 and 5.0 minutes after application, subjects rated
discomfort/sting perception on a scale from 0 (no discomfort) to
3.0 (severe discomfort) in increments of 0.5.
Results:
TRIglyphix® Sense was shown to help soothe discomfort by 59%
after five minutes, when compared to the placebo. The results
support the use of TRIglyphix® Sense in cosmetic and personal
care formulations to minimize the potential aggravation caused by
irritating cosmetic ingredients.
To determine if TRIglyphix® Sense could deliver an additional
soothing benefit to formulations that are developed specifically for
sensitive skin, (e.g. free of fragrances, dyes and actives.)
• 10 male panelists, ages 18-49
o Facial Cream (placebo)
o Facial Cream with 2% TRIglyphix® Sense
A clinician applied the placebo to one side of the panelists face
and the cream with 2% TRIglyphix® Sense to the other side
(similar to Study 1). Following 2.5 and 5.0 minutes after application,
subjects rated discomfort/sting perception on a scale from 0
(no discomfort) to 3.0 (severe discomfort) in increments of 0.5.
Results:
TRIglyphix® Sense was shown to help soothe burning and
stinging sensations by 73% after five minutes, when compared
to the placebo. This suggests TRIglyphix® Sense offers excellent
soothing properties that can help make a mild formula even
gentler to the skin.
REVITALIZATION
Rebuild & Repair
Sensitive skin is extremely susceptible to damage, specifically to harsh environmental conditions and chemical insult.
It is often prone to drying and cracking. When damage occurs, steps must be taken to prevent instances of acute
inflammation from becoming a chronic issue. Important factors for optimizing the skin’s repair include fibroblast
proliferation, collagen, elastin, hyaluronic acid synthesis and MMP-1 inhibition.
As skin ages, the integrity of its composition diminishes, resulting in thinner and less elastic skin, reduced moisture,
deeper wrinkles and a loss of cushion. TRIglyphix® Sense has been shown to stimulate ECM production for repair,*
while simultaneously delivering anti-aging benefits for sensitive skin to help target fine lines and wrinkles. Testing
has shown TRIglyphix® Sense will help to repair and revitalize injured skin.
Improvements in Fibroblast Proliferation
Improvements in Collagen Production
80
66.1
60
40
54.1
50
45.5
42.2
46.5
Percent (%) Increase
Percent (%) Increase
100
40
30
10
0
0
0.001%
TRIglyphix
Sense
0.01%
TRIglyphix
Sense
0.1%
TRIglyphix
Sense
0.0017%
Ascorbic
Acid
Treatment
Figure 7
36.4
20
20
0.0017%
Ascorbic
Acid
34.3
0.001%
TRIglyphix
Sense
Treatment
Figure 8
Studies were performed on human normal fibroblasts (HFF-1 cell line) and effects measured after 72 hours of treatment
Ascorbic acid was used as the positive control
Statistically significant compared to the untreated control (p< 0.001)
*
0.01%
TRIglyphix
Sense
Increase in Hyaluronic Acid (mg/ml)
Improvements in Hyaluronic Acid Production
10
9
8
7.4
7
6
5
4
3
4.2
3.2
2
1.6
1
0
0.0017%
Ascorbic
Acid
0.001%
TRIglyphix
Sense
0.01%
TRIglyphix
Sense
0.5%
TRIglyphix
Sense
Treatment
Figure 9
Improvements in Elastin Production
Percent (%) Increase
97.1
56.3
53.4
13.1
0.0017%
Ascorbic
Acid
0.001%
TRIglyphix
Sense
0.01%
TRIglyphix
Sense
0.1%
TRIglyphix
Sense
Treatment
Figure 10
Percent (%) Decrease
Reduction of MMP-1 Activity
55.6
53.0
51.9
0.0017%
Ascorbic
Acid
51.9
0.001%
TRIglyphix
Sense
0.01%
TRIglyphix
Sense
Treatment
Figure 11
0.1%
TRIglyphix
Sense
TRIglyphix Sense
®
A Natural Active for Sensitive Skin
KEY BENEFITS
• Effectively soothes & protects skin
• Reduces expression of pro-inflammatory biomarkers
• Shown to reduce redness, burning & stinging
• Delivers anti-aging benefits for sensitive skin formulations
SUGGESTED APPLICATIONS
Sensitive skin products:
• Facial moisturizers, serums & creams
• Cleansers
• Hand & body lotions
Shaving applications:
• Shave creams & gels
• After-shave applications
Anti-aging products:
• Facial moisturizers, serums & creams
TRI-K Industries, Inc.
•
+1 (800) 526-0372
•
info @ tri-k.com
•
www.tri-k.com
The information contained in this publication is provided in good faith and is based on our current knowledge as of the date hereof. No legally binding promise or
warranty regarding the suitability of our products for any specific use is made. Claim ideas are offered solely for your consideration, investigation and verification. TRI-K Industries, Inc. will not assume any expressed or implied liability in connection with any use of this information.
9.13