Discovering Cellular Respiration
Heather E. Bergan-Roller1†, Nicholas J. Galt2†, Joseph T. Dauer1
Tomáš Helikar3*
Affiliations:
1School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE
68583.
2Department of Science, Valley City State University, Valley City, ND 58072
3Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588
†Authors contributed equally to this work
*Corresponding Author
Tomáš Helikar
1901 Vine Street
Lincoln, NE 68588-0664
Phone: 402-472-2932
Email:
[email protected]
Homework assignment
• Please hand in your answer sheet for the
background reading. Be sure your name is on
the paper!
Discovering Cellular Respiration
Learning Goals
• Students will understand the steps of cellular
respiration.
• Students will discover how computational
modeling can be used to observe and
quantitatively measure the dynamics of biological
macromolecules involved in cellular respiration
• Students will understand how biological activity
of macromolecules are regulated
• Students will understand how cells transform
energy and cycle matter
Learning Objectives
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Students will be able to describe how changes in cellular homeostasis affect
metabolic intermediates
Students will be able to perturb and interpret a simulation of cellular respiration.
Students will be able to describe cellular mechanisms regulating cellular
respiration
Students will be able to describe how glucose, oxygen, and coenzymes affect
cellular respiration
Students will be able to describe the interconnectedness of cellular respiration
Students will be able to identify and describe the inputs and outputs of cellular
respiration, glycolysis, pyruvate processing, citric acid cycle, and electron transport
chain
Students will be able to describe how different energy sources are used in cellular
respiration
Students will be able to trace carbon through cellular respiration from glucose to
carbon dioxide
Discovering Cellular Respiration
• Matching
• Access Cell Collective and set up simulations
• 5 Investigations
A. Predict the outcome
B. Support your prediction with a mechanism
C. Test your prediction with the simulation
D. Report your simulation results
E. Evaluate your prediction based on results
(repeat A-E until you can…)
F. Accurately describe the mechanism
Mechanistic Explanation
What is a mechanistic explanation?
• Explains HOW something happens.
• Identifies the components involved in a phenomenon and HOW those components
interact.
Mechanistic Explanation
What is a mechanistic explanation?
• Explains HOW something happens.
• Identifies the components involved in a phenomenon and HOW those components
interact.
Example Question:
Considering the model below, provide an mechanistic explanation for how glucose is
involved in the activation of the citric acid cycle.
Mechanistic Explanation
What is a mechanistic explanation?
• Explains HOW something happens.
• Identifies the components involved in a phenomenon and HOW those components
interact.
Example Question:
Considering the model below, provide an mechanistic explanation for how glucose is
involved in the activation of the citric acid cycle.
Glucose is broken down during glycolysis to produce pyruvate. The pyruvate is
converted into acetyl-CoA during pyruvate processing. The acetyl-CoA then
activates the citric acid cycle.
What you’ll need to continue
A. learn.CellCollective.org
B. Discovering Cellular Respiration activity
packet
C. About 2 hours
Matching – 10 minutes
• Please work as individuals to complete the
matching activity (about 5 minutes)
• Discuss your results with your lab group
• Change your answers as needed, based on the
discussion
Access software – 10 minutes
• Go to learn.cellcollective.org
• Sign in/up
• Find and access “Discovering Cellular
Respiration”
• Enter the simulation page
• Set settings according to the instructions
• Simulate basic cellular respiration
• Compare your results with your lab group
Investigation 1 – 20 minutes
A.
B.
C.
D.
Predict outcomes
Defend your prediction
Test your prediction
Record your results
•
Compare your results with your lab group
E. Evaluate your prediction
(repeat A-E until you can…)
F. Accurately describe the mechanism
Fill out worksheet and complete
• Investigation 2 – about 20 minutes
• Investigation 3 – about 20 minutes
– Examine a lab group member’s mechanistic
prediction (part B).
• Identify and circle the components involved
• Identify and underline how the components interact
• Investigation 4 – about 20 minutes
• Investigation 5 – about 20 minutes
When you’re done
• Please hand in your answer sheet for the
activity. Be sure your name is on the paper!