MATERIAL DATA SHEET
Recombinant Human His6 UBE2N (Ubc13)/Uev1a Complex Cat. # E2­664 Ubiquitin­conjugating Enzyme E2N (UBE2N), also known as Ubiquitin­conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive Uev1a/UBE2V1 protein (1). Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. Human Uev1a/UBE2V1 shares 89% aa sequence identity with its mouse and rat orthologs. The UBE2N (Ubc13)/Uev1a Complex functions with Ubiquitin ligases (E3s), including TRAF­6 and TRIM5, to synthesize Lys63­linked Ubiquitin chains (2,3). These Ubiquitin chains can either be unanchored or attached to target proteins (2,4). The UBE2N (Ubc13)/Uev1a Complex localizes primarily to the cytoplasm and is important for NF­κB activation (5). Additionally, this complex may function in the intracellular response to retroviral capsid recognition (3). Pathologically, the UBE2N (Ubc13)/Uev1a Complex is required for the proliferation of diffuse large B­cell lymphoma cells, suggesting that it may play a role in cancer (6). Product Information
Quantity:
100 µg MW:
18 kDa (UBE2N) & 21 kDa (Uev1a) Source:
E. coli­derived Contains an terminal 6­His tag Accession # P61088 (UBE2N) & Q13404 (Uev1a)
Stock:
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM Nacl, 10% Glycerol (v/v), 2 mM TCEP. Purity:
>95%, by SDS­PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. Rev. 5/7/2014 Page 1 of 2 www.bostonbiochem.com
Boston Biochem products are available via the R&D Systems distributor network.
USA & CANADA Tel: (800) 343-7475 EUROPE Tel: +44 (0)1235 529449 CHINA Tel: +86 (21) 52380373
Use & Storage
Use:
Recombinant Human His6­UBE2N (Ubc13)/Uev1a Complex is a member of the Ubiquitin­conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin­
activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6­UBE2N (Ubc13)/Uev1a Complex concentration of 0.1­1 μM.
Storage:
Use a manual defrost freezer and avoid repeated freeze­thaw cycles.
l 12 months from date of receipt, ­70 °C as supplied. l 3 months, ­70 °C under sterile conditions after opening. Literature
References:
1. Deng, L. et al. (2000) Cell 103:351. 2. Xia, Z.P. et al. (2009) Nature 461:114. 3. Pertel, T. et al. (2011) Nature 472:361. 4. Liu, C. et al. (2009) Sci. Signal. 2:ra63. 5. Andersen, P.L. et al. (2005) J. Cell Biol. 170:745. 6. Pulvino, M. et al. (2012) Blood 120:1668. For research use only. Not for use in humans. Rev. 5/7/2014 Page 2 of 2 www.bostonbiochem.com
Boston Biochem products are available via the R&D Systems distributor network.
USA & CANADA Tel: (800) 343-7475 EUROPE Tel: +44 (0)1235 529449 CHINA Tel: +86 (21) 52380373