Preliminary results concerning the element
content of Fusarium-infected wheat
genotypes
Andrea Pető
Ph.D. student
Szeged – Timisoara axis for the safe food and feed
SZETISA1
Hungary-Romania Cross-Border Co-operation Programme 2007-2013
HURO/0901/147/2.2.2.
Timisoara, May 30-31, 2011
Materials and methods
AAS Hitachi Z-8200
Optima 7000 ICP-OES
• Sampling happened in summer of 2010
• Measuring in autumn of 2010
•Department of Physical Geography and
Geoinformatics, University of Szeged
MarsXPress CEM
Wheat sampling
1. GK Mini Manó/Nobeokabozu population
474
484
Wheat sampling
1. GK Mini Manó/Nobeokabozu population
415
493
Resistant lines
502
504
515
Sensitive lines
500
514
518
A- grain
B- chaff
C-flag leaf
D-green ear
E-culm
Sensitive: 518
504
500
493
415
Resistant: 515
502
496
484
474
10 Elements were measured:
Fe, Mn, Cu, Zn, Ni, Co, Cr, Cd, As, Se
518 sample
518 A Sensitive
60.000
80.000
50.000
70.000
60.000
μg/g
40.000
μg/g
518 B Sensitive
30.000
50.000
40.000
30.000
20.000
20.000
10.000
10.000
0.000
-10.000
Fe
Mn
Cu
Zn
Ni
Co
Cr
Cd
As
Se
518 C Sensitive
Elements
0.000
-10.000
Fe
Mn
Cu
Zn
Ni Co Cr
Elements
Cd
As
Se
As
Se
120.000
100.000
μg/g
80.000
60.000
40.000
20.000
518 D Sensitive
70.000
518 E Sensititve
0.000
-20.000
Fe Mn Cu Zn Ni Co Cr Cd As Se
Elements
25.000
60.000
50.000
20.000
40.000
15.000
μg/g
μg/g
30.000
30.000
10.000
20.000
5.000
10.000
0.000
0.000
-10.000
Fe Mn Cu Zn Ni Co Cr Cd As Se
Elements
-5.000
Fe Mn Cu
Zn
Ni
Co
Elements
Cr
Cd
The highest Mn values
484 B Resistant
493 B Sensitive
B = chaff
100.000
100.000
90.000
80.000
80.000
60.000
70.000
μg/g
μg/g
60.000
50.000
40.000
40.000
20.000
30.000
The highest Ca values
20.000
The lowest Ca values
0.000
Fe
10.000
493 B Sensitive -> 32,39 μmolCa/gDM
0.000
484
B FeResistant
-> Ni57,13
μmolCa/gDM
Mn Cu
Zn
Co
Cr
Cd
As
Se
-10.000
Mn
Cu
Zn
Ni
Co
Cr
Cd
As
Se
Cr
Cd
As
Se
500 B Sensitive -> 17,3 μmolCa/gDM
-20.000
496 B Resistant -> Elements
48,86 μmolCa/gDM
Elements
The lowest Mn values
515 B Resistant
140.000
60.000
120.000
50.000
100.000
40.000
μg/g
μg/g
80.000
60.000
500 B Sensitive
30.000
20.000
40.000
10.000
20.000
0.000
0.000
Fe
-20.000
Mn
Cu
Zn
Ni
Co
Elements
Cr
Cd
As
Fe
Se
-10.000
Mn
Cu
Zn
Ni
Co
Elements
Ca content
200
180
160
120
100
Sensitive
80
Resistant
60
40
20
0
grain
green ear
culm
chaff
flagleaf
Mn content
2.5
2
Measured by AAS
μmolMn/gDM
μmolCa/gDM
140
1.5
Sensitive
Resistant
1
0.5
0
grain
green ear
culm
chaff
flagleaf
Cu Measured by ICP
70
7
60
6
50
5
μg/g
μg/g
Summarized data Zn
40
4
30
3
20
2
10
1
0
grain
chaff
green ear
culm
0
Fe
flagleaf
grain
chaff
green ear
culm
flagleaf
140
120
μg/g
100
80
Resistant
60
Sensitive
40
20
Se
Mn
0
grain
3.5
chaff
green ear
culm
120
3
100
2.5
80
2
μg/g
μg/g
flagleaf
1.5
60
40
1
20
0.5
0
0
grain
chaff
green ear
culm
flagleaf
grain
chaff
green ear
culm
flagleaf
Ca content
Cu content
250.00
0.16
0.14
200.00
μmolCu/gDM
150.00
100.00
50.00
0.1
0.08
0.06
0.04
0.02
0.00
grain
green ear
culm
chaff
flagleaf
0
grain
green ear
culm
chaff
flagleaf
Mn content
4.5
4
3.5
μmolMn/gDM
μmolCa/gDM
0.12
3
Csillag Infected
2.5
Kalász Infected
2
Csillag Not Infected
1.5
Kalász Not Infected
1
0.5
0
grain
green ear
culm
chaff
flagleaf
Measured by AAS
R - Resistant
S - Sensitive
Element content
250
200
150
Zn
Cu
Se
100
Mn
Fe
50
0
R
S
chaff
R
S
grain
R
S
green ear
R
S
culm
R
S
flagleaf
Measured by ICP
Our 0-hour-old samples - May, 2011
Culms, flagleaves and ears
According to earlier results:
474 – Resistant – high Mn content
483 – Sensitive – high Mn content
488 – Sensitive – low Mn content
501 – Resistant – low Mn content
Thank you for your attention!