Supplementary information
Fig. S1
60

miR-10b (fold)
50
40
30
20
10
0
231 vector media
231 miR-10b stable media
Fig S1. miR-10b overexpression in exosomes derived
from MDA-MB-231 cells stably expressing miR-10b
as compared to vector control exosomes.
Table S1.Endogenous level of miRNA expression in MDA-MB-231 cells as compared to HMLE cells
miRNA
hsa-let-7a
hsa-miR-1
hsa-miR-17
hsa-miR-22
hsa-miR-29a
hsa-miR-32
hsa-let-7b
hsa-miR-7
hsa-miR-18a
hsa-miR-23a
hsa-miR-29b
hsa-miR-34a
hsa-let-7c
hsa-miR-9
hsa-miR-18b
hsa-miR-23b
hsa-miR-29c
hsa-miR-34b
hsa-let-7d
hsa-miR-10a
hsa-miR-19a
hsa-miR-24
hsa-miR-30a
hsa-miR-34c-5p
hsa-let-7e
hsa-miR-10b
hsa-miR-19b
hsa-miR-25
hsa-miR-30b
hsa-miR-92a
hsa-let-7f
hsa-miR-15a
hsa-miR-20a
hsa-miR-26a
hsa-miR-30c
hsa-miR-92b
hsa-let-7g
hsa-miR-15b
hsa-miR-20b
hsa-miR-26b
hsa-miR-30e
hsa-miR-96
hsa-let-7i
hsa-miR-16
hsa-miR-21
hsa-miR-27b
hsa-miR-31
hsa-miR-98
Fold change
1.59
3.37
0.81
1.60
2.14
1.96
0.99
0.55
0.72
1.02
2.60
2.38
1.50
1.57
0.68
0.96
2.17
0.38
1.69
9.53
0.83
0.80
1.72
0.53
1.64
13.21
0.97
1.64
1.21
0.85
1.47
1.56
0.85
1.27
2.10
0.59
1.35
1.04
0.75
1.12
1.85
0.81
2.56
1.16
1.87
1.14
0.53
1.44
SEM
0.21
1.23
0.17
0.25
0.55
0.01
0.14
0.06
0.11
0.08
0.23
0.33
0.24
0.26
0.12
0.15
0.18
0.09
0.23
1.47
0.11
0.11
0.20
0.01
0.21
1.51
0.15
0.23
0.21
0.06
0.22
0.23
0.15
0.23
0.23
0.15
0.14
0.14
0.08
0.22
0.31
0.13
0.17
0.07
0.17
0.02
0.53
0.50
miRNA
hsa-miR-99a
hsa-miR-100
hsa-miR-101
hsa-miR-103
hsa-miR-106a
hsa-miR-106b
hsa-miR-107
hsa-miR-122
hsa-miR-124
hsa-miR-125a-5p
hsa-miR-125b
hsa-miR-126
hsa-miR-126*
hsa-miR-127-5p
hsa-miR-128
hsa-miR-129-5p
hsa-miR-130a
hsa-miR-133a
hsa-miR-133b
hsa-miR-134
hsa-miR-135a
hsa-miR-135b
hsa-miR-136
hsa-miR-137
hsa-miR-138
hsa-miR-140-5p
hsa-miR-141
hsa-miR-142-5p
hsa-miR-142-3p
hsa-miR-143
hsa-miR-144
hsa-miR-145
hsa-miR-146a
hsa-miR-146b-5p
hsa-miR-148a
hsa-miR-148b
hsa-miR-149
hsa-miR-150
hsa-miR-151-5p
hsa-miR-153
hsa-miR-154
hsa-miR-155
hsa-miR-181a
hsa-miR-181b
hsa-miR-181c
hsa-miR-181d
hsa-miR-182
hsa-miR-183
Fold change
9.09
7.41
1.04
1.16
0.78
1.76
1.12
1.10
0.55
SEM
0.75
3.75
0.09
0.26
0.10
0.13
0.10
0.21
0.08
1.43
4.56
5.04
3.95
0.72
0.92
0.48
1.13
1.32
1.05
4.55
0.28
0.24
0.11
2.45
4.26
3.35
0.00
4.43
9.04
0.84
4.04
1.11
2.04
5.70
0.54
0.62
0.90
0.48
1.03
2.05
0.07
0.03
2.09
2.49
2.45
2.61
0.67
1.01
0.30
0.33
1.20
0.61
0.22
0.09
0.48
0.11
0.11
0.26
0.66
0.05
0.04
0.05
0.40
0.77
0.48
0.00
0.57
0.41
0.08
1.58
0.08
0.92
1.32
0.08
0.07
0.16
0.03
0.13
0.59
0.01
0.01
0.27
0.28
0.30
0.40
0.05
0.01
miRNA
hsa-miR-184
hsa-miR-185
hsa-miR-186
hsa-miR-188-5p
hsa-miR-190
hsa-miR-191
hsa-miR-192
hsa-miR-193a-5p
hsa-miR-193b
hsa-miR-194
hsa-miR-195
hsa-miR-196a
hsa-miR-196b
hsa-miR-199a-5p
hsa-miR-199a-3p
hsa-miR-199b-5p
hsa-miR-200a
hsa-miR-200b
hsa-miR-200c
hsa-miR-202
hsa-miR-203
hsa-miR-204
hsa-miR-205
hsa-miR-206
hsa-miR-210
hsa-miR-212
hsa-miR-214
hsa-miR-215
hsa-miR-216a
hsa-miR-218
hsa-miR-219-5p
hsa-miR-220a
hsa-miR-221
hsa-miR-222
hsa-miR-223
hsa-miR-224
hsa-miR-328
hsa-miR-296-5p
hsa-miR-301a
hsa-miR-335
hsa-miR-342-5p
hsa-miR-363
hsa-miR-372
hsa-miR-373
hsa-miR-375
hsa-miR-376a
hsa-miR-378
hsa-miR-380
Fold change
1.55
0.89
0.93
0.55
1.53
1.37
2.85
1.43
0.18
1.91
1.16
0.14
0.43
1.77
0.62
0.70
1.96
1.15
0.02
1.45
0.00
4.73
0.00
0.76
1.68
1.84
0.88
1.47
1.43
12.68
0.82
3.85
4.97
3.76
0.17
2.70
0.60
0.43
1.89
4.10
0.35
5.35
1.20
1.25
0.75
0.00
0.41
0.81
SEM
0.42
0.22
0.12
0.08
0.47
0.17
0.51
0.24
0.02
0.37
0.14
0.00
0.13
0.42
0.24
0.22
0.36
0.22
0.00
0.20
0.00
0.92
0.00
0.03
0.21
0.32
0.06
0.19
0.19
1.44
0.13
1.25
3.60
2.14
0.16
0.76
0.22
0.33
1.25
1.21
0.11
0.11
0.29
0.41
0.15
0.00
0.07
0.25
Table S2. Secretory miRNA expression in MDA-MB-231 supernatant as compared to HMLE supernatant
miRNA
hsa-let-7a
hsa-miR-1
hsa-miR-17
hsa-miR-22
hsa-miR-29a
hsa-miR-32
hsa-let-7b
hsa-miR-7
hsa-miR-18a
hsa-miR-23a
hsa-miR-29b
hsa-miR-34a
hsa-let-7c
hsa-miR-9
hsa-miR-18b
hsa-miR-23b
hsa-miR-29c
hsa-miR-34b
hsa-let-7d
hsa-miR-10a
hsa-miR-19a
hsa-miR-24
hsa-miR-30a
hsa-miR-34c-5p
hsa-let-7e
hsa-miR-10b
hsa-miR-19b
hsa-miR-25
hsa-miR-30b
hsa-miR-92a
hsa-let-7f
hsa-miR-15a
hsa-miR-20a
hsa-miR-26a
hsa-miR-30c
hsa-miR-92b
hsa-let-7g
hsa-miR-15b
hsa-miR-20b
hsa-miR-26b
hsa-miR-30e
hsa-miR-96
hsa-let-7i
hsa-miR-16
hsa-miR-21
hsa-miR-27b
hsa-miR-31
hsa-miR-98
Fold change
1.00
1.38
0.60
1.05
2.58
5.00
0.88
1.63
1.70
0.74
1.00
3.25
0.60
1.09
2.18
1.41
0.75
0.89
1.21
2.75
1.37
0.64
0.46
1.50
4.13
5.02
0.91
1.98
1.35
1.07
2.23
1.39
1.03
0.39
4.96
1.89
1.36
1.06
0.68
1.81
0.98
1.79
1.34
0.94
0.92
2.13
0.45
1.98
SEM
0.62
0.79
0.16
0.56
1.51
2.83
0.30
0.08
0.81
0.14
0.12
2.94
0.14
1.09
2.17
1.02
0.06
0.00
0.33
0.59
0.40
0.39
0.30
0.67
1.93
0.48
0.38
1.46
0.59
0.81
1.23
0.51
0.45
0.13
2.45
1.02
0.90
0.28
0.20
0.81
0.09
0.38
0.34
0.06
0.50
1.33
0.43
0.98
miRNA
hsa-miR-99a
hsa-miR-100
hsa-miR-101
hsa-miR-103
hsa-miR-106a
hsa-miR-106b
hsa-miR-107
hsa-miR-122
hsa-miR-124
hsa-miR-125a-5p
hsa-miR-125b
hsa-miR-126
hsa-miR-126*
hsa-miR-127-5p
hsa-miR-128
hsa-miR-129-5p
hsa-miR-130a
hsa-miR-133a
hsa-miR-133b
hsa-miR-134
hsa-miR-135a
hsa-miR-135b
hsa-miR-136
hsa-miR-137
hsa-miR-138
hsa-miR-140-5p
hsa-miR-141
hsa-miR-142-5p
hsa-miR-142-3p
hsa-miR-143
hsa-miR-144
hsa-miR-145
hsa-miR-146a
hsa-miR-146b-5p
hsa-miR-148a
hsa-miR-148b
hsa-miR-149
hsa-miR-150
hsa-miR-151-5p
hsa-miR-153
hsa-miR-154
hsa-miR-155
hsa-miR-181a
hsa-miR-181b
hsa-miR-181c
hsa-miR-181d
hsa-miR-182
hsa-miR-183
Fold change
2.88
1.95
0.22
0.46
0.22
2.92
0.30
0.68
2.22
0.52
1.06
1.00
0.68
0.78
0.38
1.14
4.21
1.58
0.50
5.66
0.75
0.80
0.78
0.51
4.29
0.96
1.18
0.68
0.68
0.29
0.40
0.78
1.10
0.00
1.00
7.71
1.21
2.11
1.00
0.87
0.13
0.50
0.29
0.88
0.47
0.38
0.78
2.89
SEM
1.65
0.11
0.22
0.30
0.01
0.40
0.30
0.12
0.73
0.13
0.35
0.00
0.12
0.22
0.18
0.32
4.07
1.02
0.50
5.66
0.01
0.03
0.22
0.49
0.03
0.71
0.01
0.12
0.12
0.27
0.40
0.22
1.10
0.00
0.00
6.71
1.21
2.11
0.00
0.13
0.13
0.50
0.14
0.48
0.08
0.06
0.22
1.89
hsa-miR-184
hsa-miR-185
hsa-miR-186
hsa-miR-188-5p
hsa-miR-190
hsa-miR-191
hsa-miR-192
hsa-miR-193a-5p
hsa-miR-193b
hsa-miR-194
hsa-miR-195
hsa-miR-196a
hsa-miR-196b
hsa-miR-199a-5p
hsa-miR-199a-3p
hsa-miR-199b-5p
hsa-miR-200a
hsa-miR-200b
hsa-miR-200c
hsa-miR-202
hsa-miR-203
hsa-miR-204
hsa-miR-205
hsa-miR-206
hsa-miR-210
hsa-miR-212
hsa-miR-214
hsa-miR-215
hsa-miR-216a
hsa-miR-218
hsa-miR-219-5p
hsa-miR-220a
hsa-miR-221
hsa-miR-222
hsa-miR-223
hsa-miR-224
hsa-miR-328
hsa-miR-296-5p
hsa-miR-301a
hsa-miR-335
hsa-miR-342-5p
hsa-miR-363
hsa-miR-372
hsa-miR-373
hsa-miR-375
hsa-miR-376a
hsa-miR-378
hsa-miR-380
Fold change
0.67
0.59
0.63
1.43
0.67
0.54
0.50
1.53
0.91
0.28
0.19
0.99
2.71
0.67
0.67
0.28
0.43
0.51
0.53
0.65
0.39
0.89
0.0027
2.34
0.67
2.90
0.64
0.89
0.47
0.89
0.28
0.89
0.69
1.24
0.50
0.76
1.99
0.40
0.89
0.28
0.78
0.67
0.74
0.67
0.66
0.89
0.31
1.29
SEM
0.11
0.41
0.15
1.10
0.11
0.22
0.50
0.83
0.80
0.28
0.04
0.42
0.65
0.11
0.11
0.28
0.13
0.26
0.47
0.35
0.10
0.11
0.002
0.34
0.45
1.21
0.27
0.11
0.22
0.11
0.28
0.11
0.52
0.88
0.50
0.24
1.59
0.37
0.11
0.28
0.22
0.11
0.35
0.11
0.16
0.11
0.31
0.29