Sysmex Users Group Meeting
Houston, TX
October 2016
SP10
© 2015 Sysmex America, Inc. All rights reserved.
OVERVIEW
Customer Input and Feedback
SP10 and SP1000 Comparison
Stain Troubleshooting
Common Errors
Operation mode options
Tips and Tricks
Open Discussion
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HAND OUT OF FEEDBACK CARDS
Common Issues by Priority
Stain Quality
Mechanical Failure
Tube Clamp Errors
Cassette Feed out/in errors
Overflow of cassettes
False Slide/Cassette on the line message
Piercer clogging, aspiration issues
Other, please describe
Reagent Issues (DI Water, Stain consistency, contamination,
etc)
Operator Training
© 2015 Sysmex America, Inc. All rights reserved.
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SP10 AND SP1000 COMPARISON
SP-10 is a minor-change product from SP-1000i
Basic specifications are same
Cost, Size, Throughput, etc.
Same footprint as SP-1000
SP-10 has some differences
Improved specifications
Additional functions
Countermeasures to quality issues
New Staining setup options over SP-1000
Improvements to Micro Tube Mode
New Printer Assembly
© 2015 Sysmex America, Inc. All rights reserved.
NEW FEATURES
1) Micro Mode
Improved time over SP-1000i
Access without opening unit
2) Stain Dilution Options
Stain 1 - Select from [1:5] or [1:10]
Stain 2 - Select from [1:9], [1:13], [1:19], or [1:25]
3) Fan Settings – more options for smear dry ; Not Use, Low, Medium, High
4) Cassette and Mechanical Re-Design
Prevents cassettes “sticking” together
Load opposite from SP-1000i
Not compatible with current system
© 2015 Sysmex America, Inc. All rights reserved.
IMPROVEMENTS
Position of Power SW
Moved to upper
side
Racks may be removed
without stress
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IMPROVEMENTS
Stain Line Cover
Customer may open the cover without screw driver
© 2015 Sysmex America, Inc. All rights reserved.
MICRO-TUBE DEAD SAMPLE VOLUME
REDUCED
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MICRO-TUBE PREPARATION TIME REDUCED
Waiting time reduced from 3 min to 30 sec
Additional cover for micro-tube on front-cover
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DEAD SAMPLE VOLUME REDUCED
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ERROR RECOVERY (SUMMARY)
Error Recovery time for stain line has been reduced
SP-1000i does not execute stain process without testing with
new cassette after all cassette has been exhausted
SP-10 can continue stain process by error recovery using
cassette in the stain line
SP-10 has new category “recovery slide” for slide status, and
customer can select error recovery process “error” and
“recovery slide” for timed out samples
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ADDITIONAL FUNCTIONS
Selectable dilution for stain/buffer solution
• Size of DP for Buffer and Stain solution is same as SP-1000i
Stain 1 Dilution Stain DP Dispense
1:10
1x
1:5
2x
Stain 2 Dilution
1:9
1:13
1:19
1:25
Buffer DP Dispense
2 x
2x
3x
2x
2x
1x
2x
2x
3x
2x
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STARTUP AND SHUTDOWN IMPROVEMENT
Startup and Shutdown sequence are shortened a
little, especially Startup.
The required number of cassettes needed for
startup and shutdown sequence is decreased:
Startup
9 down to 7
Shutdown1 13 down to 11
Shutdown2 9 down to 8
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PIERCER RINSING DURING SHUTDOWN
SP-10 aspirates CELLCLEAN 8 times from the sample tube
via piercer to waste from dispense pipette on Shutdown 1
and 2 sequence
It will reduce clogging and contamination in piercer line
*Consumption of CELLCLEAN will increase from 300uL to
4000uL
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CHANGE FROM 2 HAND TO 1
Change hand number of clipper
hands from 2 to 1
Reduces sample tube
handling issues
Throughput will not change on
normal order ratio
Challenging for R&D to
increase throughput on low
order ratio
*2-hands will be needed for
stand-alone systems and old
system connection
© 2015 Sysmex America, Inc. All rights reserved.
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TIP AND TRICKS
• Poor stain quality or “2-tone” staining, smear washing off
slide.
• Most likely cause is a partially occluded stain pipette 1 at the tip.
• During slow periods, residual stain on interior of pipette collects
at the tip and dries up causing an occlusion.
• If unable to insert paper clip, remove “thumb screw” holding
pipette assembly and remove occlusion
• Suggest bending large paper clip into a “fish hook” and attempt
to insert it into the bottom of the pipette.
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CASSETTE OVERFLOWING AT STAIN PIPETTE OR
CASSETTE FEED OUT ERROR AT PIPETTE 2
 Possible cause is long pipette at pipette 2 is
occluded or there is insufficient vacuum at the
stain chambers.
 Suggest verifying no occlusion at tip of long
pipette.
 Suggest verifying vacuum supply port on top
of the stain chambers is not occluded.
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CASSETTE FEED OUT ERROR AT DRY
POSITION
• Usually because cassette is overflowing at rinse pipette
just a drop or two. Cassette is cracked and leaking.
Cassettes not completely dried after washing.
• Causes cassettes to “stick” together by capillary action
• Error unable to recover until the liquid dries up
• Not necessary to manually remove cassette under
pipettes.
• Suggest attempt to recover from error and when belt
begins to move push cassette toward the back to “unstick” from adjacent cassette.
• “Remove slides and Cassettes” does not refer to stain
table.
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REMOVE SLIDE OR CASSETTE FROM TABLE
•
•
•
Little windows on slide table are dirty causing slide sensor to be
activated.
Suggest cleaning little windows and/or checking sensor status screen to
see which sensor is being falsely activated.
This message does not refer to slides or cassettes below stain pipettes.
© 2015 Sysmex America, Inc. All rights reserved.
STAIN CHAMBER NOT FILLED
• At Startup: Possible kinked stain tubing above stain bottle
• At Shutdown: Possible kinked tubing above methanol bottle
• Possible poor vacuum supply at stain
chambers, possible vacuum supply port
occluded above stain chambers
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SMEAR APPEARS TO BE WASHED OFF
• Possible buffer tubing kinked above buffer bottle
causing no dilution to be made and delivered to
cassette at stain pipette 2. Possibly tubing is
above the float switch in the buffer bottle.
• Buffer not being delivered into Mixing Chamber
• Concentrated DST installed instead of DCL
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FEEDBACK CARD REVIEW AND DISCUSSION
Common issue 1?
Common issue 2?
Common issue 3?
Common issue 4?
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