# 842, II Floor, Sahakar Bhavan,
A Block, Sahakarnagar
Bangalore – 560092
080- 23620050  080-23625647  [email protected]/[email protected]
Sequencing Requisition Form
Date
Name
Institute/Organization
Address
Phone
Email
Sample Requirements & Instructions:
Please indicate whether the sample is Purified or Unpurified
Purified Plasmids:
 1 or 2g (for every 500-600 bp read) , either in dried or in 10/20l of Tris Hcl,pH 8.0-9.0.
 Minimum template conc. should be 100 ng/l and minimum volume should be 10l.
 Provide 0.5g of more DNA for every additional reactions.
Unpurified Plasmids:
 Please provide 4 or 5g, either in dried or in Tris Hcl,pH 8.0-9.0.
Purified PCR Products:
 30 ng DNA per 100bp product length , either in dried or in 20l Tris-Cl, pH 8.0.
 Minimum template conc. should be 100 ng/l and minimum volume should be 10l.
 Provide 20ng more DNA per 100 bp product length for every additional reactions.
Unpurified PCR Products:
 Please provide 4 or 5g , either in dried or in Tris Hcl,pH 8.0-9.0.
Primer:
 Primer conc. shoud be 10pmol/l, and minimum volume of 10 l.
 Provide 5l of more primer for every additional reactions.
 Enclose the sequence of your primers in the provided column
Plasmid prepration:
 Provide E. coli colonies on plate or pure stab culture in presence of antibiotic as selection marker.
 Provide vector name, size, copy number and antibiotic name as a selection marker.
Special Instructions:
 Please submit samples in 1.5ml micro centrifuge tubes.
 Please do not use Tris EDTA buffer for eluting/dissolving your samples.
 Please indicate if your samples have high GC content, repeats… in the special instruction column provided
Sample Collection: Tuesday & Friday (in every week)
Please Call: 011-41751641 or mail: [email protected]
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Primer Details
Note: Not required for standard Sequencing Oligos
Sl No.
Sequence (5’ - 3’)
Provided
(Yes/NO)
Concentration
(pmol/l)
1
2
3
Sample Details:
Sl No.
Sample Type
(Plasmid/PCR)
Sample Name
Vector
Insert/ Product
Size ( kb)
Concentration
of Plasmid/
PCR Product
Primers To Be Used
Cat. No.
Special Instructions if any:
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