Classifying macroinvertebrates (Years 7 and 8) Lesson plan Introduction Macroinvertebrates (waterbugs) are a key indicator of waterway health. This series of lessons focuses on scientific classification of organisms. Students classify macroinvertebrates by their observable structural features and highlight the biodiversity that can be found in freshwater environments. Students develop dichotomous key using their own criteria for classifying macroinvertebrates and consider how biologists use keys in their field work. Activity 1: Identifying macroinvertebrates Victorian Curriculum F–101 links: Levels 7 and 8 Science Science Understanding Science as a Human Endeavour Science and technology contribute to finding solutions to a range of contemporary issues; these solutions may impact on other areas of society and involve ethical considerations Biological sciences There are differences within and between groups of organisms; classification helps organise this diversity Students identify macroinvertebrates in a water sample and consider how these organisms can be used to indicate the ecological health of a waterway. Activity 2: Classifying macroinvertebrates Students explore how organisms are classified and devise a dichotomous key for macroinvertebrates. 1 Victorian Curriculum and Assessment Authority (VCAA) <http://victoriancurriculum.vcaa.vic.edu.au/> Accessed 14 August 2016. Activity 1: Identifying macroinvertebrates Students identify macroinvertebrates in a water sample and consider how these organisms can be used to indicate the ecological health of a waterway. Equipment Waterbug collection equipment—net, pole, bucket and gloves Images of the waterway sampling site One bucket of water with a macroinvertebrate sample from local waterway For each group: One copy of the A3 laminated Macroinvertebrates ID chart (attached) and/or access to an online guide such as A beginner’s guide to waterbug identification or a hardcopy version from <http://www.waterwatchmelbourne.org.au/content/volunteer_monitoring/monitoring_ water_bugs_(macroinvertebrates)/monitoring_water_bugs_(macroinvertebrates).asp > One copy of the Macroinvertebrate data form (download from <http://www.waterwatchmelbourne.org.au/content/library/Macroinvertebrate_Data_Fo rm.pdf> Waterbug sorting and viewing equipment—one white catering tray for sorting, an ice cube tray, five spoons, magnifying glasses Digital microscope and interactive whiteboard or data projector, if possible Preparation Collect macroinvertebrate sample from a healthy section of a waterway. Take a photo to show students where the sample came from. After the lesson, make sure that you take them back to the same place you collected them. Ensure that all safety requirements are followed. Activity steps 1. Ask students to list animals that live in a waterway. Once students have done so, pinch your thumbs and forefingers together to make a small diamond. Explain to students the animals we will be looking at are on average the size of the space between your fingers. 2. Explain that these animals support the food chain, and without them the turtles, platypus, fish, birds and frogs wouldn’t be able to survive. 3. Introduce the term ‘aquatic macroinvertebrates’ or waterbugs. Explain that this term refers to the fact that these animals live in water and have no backbone. 4. Tell students that you visited a local waterway and sampled it for macroinvertebrates. Show students the image of the waterway you sampled. Explain where and how you collected the sample. In your description, explain the terms riparian vegetation; verge; riffle; aquatic plants; slow-flowing, deep pools; muddy, sandy or rocky bottom. A drawing, diagram or enlarged, laminated photographs may be useful. 5. Display the Scope Waterbugs video [3:13] found on the Melbourne Water website at <http://www.melbournewater.com.au/getinvolved/protecttheenvironment/Pages/Waterbu g-Census.aspx> 6. Show students the water samples and explain that they will be looking at the macroinvertebrates. Explain how to look for macroinvertebrates in the sample and how to identify them using the Macroinvertebrate ID charts or the A beginner’s guide to waterbug identification. Use this opportunity to demonstrate how to care for the live organisms ethically i.e. using spoons for carefully lifting the macroinvertebrates from the sample, disturbing them as little as possible. 7. Demonstrate how to use equipment correctly. For example: using the spoon to carefully lift the macroinvertebrates from the sample into the ice cube trays for observation using magnifying glasses to look for features on our macroinvertebrates using the identification charts (with photos) to help identify common macroinvertebrates not stirring up the sample—it makes it harder to see the macroinvertebrates! 8. Organise students into small groups. Explain that they will be looking for macroinvertebrates in a tray. Assign the groups a tray to look at. 9. Using the naked eye or magnifying glasses, students will make some observations of the sample of macroinvertebrates such as: the number of different types the numbers of each type the size, shape, colour of the macroinvertebrates features such as legs, tail, wings or mouth parts. If possible, use a digital microscope to display macroinvertebrates using a magnification of 10X. Students draw a labelled diagram of each macroinvertebrate in their notebooks showing the shape and any distinguishing features such as legs, tail, wings, shells or mouth parts etc. These drawings can be used in Activity 2. 10. Explain how to complete the Macroinvertebrate data form. 11. Add macroinvertebrate sample to catering trays with five or so students at each tray. Provide each group with a macroinvertebrate data form to complete. Remind students of time limits during activity. Assist students as necessary with identification. 12. Collate the macroinvertebrate data forms on the class data sheet (or whiteboard). Discuss sensitivity of macroinvertebrates that were found and what they indicate about the water quality of the local waterway. What factors would affect the health of the waterway? 13. Explain that in the next lesson we will explore how biologists classify macroinvertebrates into groups. 14. Ask students what kind of actions can they take to help protect their waterways? Examples: Increased nutrients affect water quality. Therefore you need to pick up dog poo when walking your dog as it enters waterways via stormwater. Make sure litter goes in the bin as litter can enter waterways via stormwater. If you like to see a range of animals around your local waterway, do your bit to limit water pollution. Look after the small macro-invertebrates and you are looking after the larger animals. Plant trees to increase shade and oxygen levels. Key messages Our waterways support a diverse range of plant and animal life, including native fish, frogs and platypuses. They are worth looking after. Everyone can help improve local rivers and creeks by reducing stormwater pollution – simple things like picking up after your dog, binning rubbish and fixing oil leaks in your car can reduce the impacts. 15. Collect macroinvertebrate samples so they can be returned to place they were collected. Activity 2: Classifying macroinvertebrates Students use macroinvertebrates to explore how biologist use classification levels to identify species. Equipment Interactive whiteboard or data projector, if possible For each group: One copy of the A3 laminated Macroinvertebrates ID chart (attached) and/or access to an online guide such as A beginner’s guide to waterbug identification or a hardcopy version from <http://www.waterwatchmelbourne.org.au/content/volunteer_monitoring/monitoring_ water_bugs_(macroinvertebrates)/monitoring_water_bugs_(macroinvertebrates).asp > One set of Macroinvertebrate cards (Attached) One sheet of paper Activity steps 1. Explain that biologists need to be able identify each type of organism and to do this they group all living things on Earth into the following categories. Kingdom Phylum Sub-phylum Class Order Family Genus Species 2. Taxonomy is the science of classifying animals. Display the following table of the major taxonomic levels. 3. 7. Level Kingdom 4. 5. 6. 8. Classification of Humans Animalia Phylum Chordata Sub-phylum Vertebrata Class Mammalia Order Primates Family Hominidae Genus Homo Species sapiens 3. Explain to students vertebrates (animals with backbones) include birds, amphibians, mammals, fish and reptiles. All others are considered invertebrates. 4. Explain to the students how we can use a key to identify the macroinvertebrates. Dichotomous means divided in two parts or classifications. With a dichotomous key, there are at least two options to choose from. 5. Display the Virtual sampling learning object at <http://lrrpublic.cli.det.nsw.edu.au/lrrSecure/Sites/LRRView/7396/applets/virtual_dip/virtu al_sampling.html> to show how macroinvertebrates can be identified with a dichotomous key. 6. Working in groups, students choose drawings of four or five different macroinvertebrates they observed in Activity 1 to devise a dichotomous key to identify these macroinvertebrates. Alternatively, distribute copies of the Macroinvertebrate cards to each group. Students select the criteria that can distinguish each of the organisms they are working with. 7. Model how students might use the criteria such as: legs/no legs tail prongs/no tail prongs gills for breathing/no gills for breathing. 8. On a sheet of paper, students create a flow chart of their classification with each branch of the flow chart separating the macroinvertebrates with a question. Questions should focus on features: for example ‘Does it have six legs?’ rather than ‘Is it an insect?’ Students continue with their questions to separate the macro-invertebrates. 9. Groups share their classification flow charts with the class. Students combine their ideas to produce a final flow chart. Discuss why field biologists commonly use dichotomous keys to identify organisms rather than an identification chart or guide. Teacher background These classification levels were devised by Carolus Linnaeus (1707–1778), a Swedish botanist, zoologist and physician. The classification levels become more specific towards the bottom, more general towards the top. Many organisms belong to the same kingdom but fewer belong to the same phylum and so on as you go down the taxonomic levels. Two animals that belong to the same family and genus are more closely related than two animals that only belong to the same family. You can learn a lot from the scientific name, e.g. Homo sapiens: Homo = self or same, meaning same as me = humans sapiens = wise Therefore, Homo sapiens means wise human. Macroinvertebrate cards
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