Deficient Transcription of Mouse Mast Cell Protease 4
Gene in Mutant Mice of mi/mi Genotype
by Tomoko Jippo, Young-Mi Lee, Yee Katsu, Kumiko Tsujino, Eiichi Morii, Dae-Ki
Kim, Hyung-Min Kim, and Yukihiko Kitamura
Blood
Volume 93(6):1942-1950
March 15, 1999
©1999 by American Society of Hematology
Mast cells expressing MMCP-4 mRNA in the skin of +/+,mi/mi, and tg/tg mice.
Tomoko Jippo et al. Blood 1999;93:1942-1950
©1999 by American Society of Hematology
Reduced expression of MMCP-4 mRNA in mi/mi andtg/tg CMC and normalization of the MMCP-4
expression inmi/mi CMC by the introduction of the +-MITF cDNA but not ofmi-MITF cDNA.
Tomoko Jippo et al. Blood 1999;93:1942-1950
©1999 by American Society of Hematology
Comparison of the structure of MMCP-4 transcript among KiSV-MC1 cells derived from a DBA/2
mouse, CMC derived from C57BL/6-+/+ mice and the mastocytoma cells derived from a LAF1
mouse.
Tomoko Jippo et al. Blood 1999;93:1942-1950
©1999 by American Society of Hematology
Nuclear run-on analysis of +/+ and mi/mi CMC.32P-labeled nuclear transcripts obtained from +/+
CMC (left lane) and mi/mi CMC (right lane) were examined for their ability to hybridize to DNA
probes for β-actin, MMCP-4, MMCP-5, and MMCP-6. pBS-derived DNA was...
Tomoko Jippo et al. Blood 1999;93:1942-1950
©1999 by American Society of Hematology
Expression of cathepsin G mRNA in +/+ and mi/miCMCs.
Tomoko Jippo et al. Blood 1999;93:1942-1950
©1999 by American Society of Hematology
The nucleotide sequence of 5′ flanking region of the MMCP-4 gene.
Tomoko Jippo et al. Blood 1999;93:1942-1950
©1999 by American Society of Hematology
Effect of coexpression of cDNA encoding +-MITF ormi-MITF on luciferase activity.
Tomoko Jippo et al. Blood 1999;93:1942-1950
©1999 by American Society of Hematology
(A) Expression of MMCP-4 mRNA in IC-2 cells.
Tomoko Jippo et al. Blood 1999;93:1942-1950
©1999 by American Society of Hematology
Binding of +-MITF to the CATGTG motif in 5′ flanking region of the MMCP-4 gene.
Tomoko Jippo et al. Blood 1999;93:1942-1950
©1999 by American Society of Hematology