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R N A I S O L AT I O N
Highest Quality mRNA
in a Single Round of Selection
Poly(A)Purist™
High quality mRNA selection requires both efficient removal of rRNA and specific recovery of poly(A) RNA.
The Poly(A)Purist Kits (patent pending) provide a level of enrichment unmatched by other protocols. The
rRNA contamination that plagues mRNA enrichment is not exclusively caused by nonspecific adsorption to
the oligo(dT) matrix but also by rRNA binding to, and co-purifying with, mRNA. Researchers at Ambion have
developed hybridization reagents and a protocol to minimize this unwanted interaction while still promoting efficient oligo(dT) selection.
This patent pending methodology has been incorporated into the new Poly(A)Purist, MicroPoly(A)Purist
and Poly(A)Purist MAG Kits. The result is an mRNA selection procedure that typically requires only a single
round of selection, yet yields RNA that is highly enriched and devoid of rRNA.
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Greater enrichment of mRNA of any available kit
Saves time—single round of selection sufficient for most applications
Fast processing & scalability with magnetic bead-based selection kit
High throughput processing on robotic platforms
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Figure 1. Poly(A)Purist™ Reduces rRNA Contamination. Poly(A)
RNA was enriched from replicate 100 µg samples of total RNA using
MicroPoly(A)Purist, Poly(A)Purist MAG, and four other commercially available poly(A) RNA selection kits. One quarter of each recovered mRNA
sample was analyzed by formaldehyde agarose gel electrophoresis. The
mRNA selected with Poly(A)Purist and Poly(A)Purist MAG exhibits very
low residual rRNA contamination.
0.5
Figure 2. Poly(A)Purist™ Recovers More mRNA. The RNA in the
formaldehyde agarose gel shown in Figure 1 was transferred to a nylon
membrane and hybridized with 32P-labeled RNA probes for β-actin,
GAPDH, and cyclophilin (respective transcript sizes are 2.1 kb, 1.5 kb,
0.7 kb). A comparison of the signals obtained for each sample demonstrates that the mRNA isolated with the Poly(A)Purist Kits yield more
mRNA than do competitors’ kits.
For more information visit www.ambion.com/prod/polyapurist
An Applied Biosystems Business
PFD.PolyA.qxd
5/1/06
5:05 PM
Page 2
Poly(A)Purist™
Superior Performance
The superior performance of Poly(A)Purist is clearly demonstrated in Figures 1 and 2 (on reverse) which consists of a comparison of the mRNA
recovered using four commercial kits and the MicroPoly(A)Purist and Poly(A)Purist kits. Identical total RNA samples were processed with each
kit and careful evaluation of the subsequent mRNA revealed that the two Ambion kits provided the lowest level of residual rRNA contamination.
Ethidium bromide staining on a denaturing agarose gel and Northern analysis confirmed the observations.
These Poly(A)Purist kits are available in two formats—one uses oligo(dT) cellulose-based selection: Poly(A)Purist and MicroPoly(A)Purist, and
one that utilizes oligo(dT) magnetic bead-based purification: Poly(A)Purist MAG kit. Both selection formats produce the highest possible yield
of exceptionally clean poly(A) RNA.
Poly(A)Purist and MicroPoly(A)Purist Kits
• Circumvent clogged columns and slow flow rates by using
batch binding method
• Save time with only a single round of selection
• Two convenient formats: six large scale isolations or
twenty small scale isolations
• Small cell or tissue samples can be processed using
MicroPoly(A)Purist
Poly(A)Purist MAG and Poly(A)Purist MAG-96 High
Throughput mRNA Kits
• Save time—whole procedure can be completed as little as
45 minutes
• Increased flexibility—process between 100 Ķg to 1 mg of
total RNA per prep
• Ease of handling with magnetic bead-based selection
• High processivity with high throughput automated kit
Poly(A)Purist™
Poly(A)Purist™ MAG
Bind
30 min.
Bind
Wash
15 min.
Capture
Elute
5 min.
Precipitate
Rinse
30 min.
15 min.
Precipitate
1 hr-ON
ORDERING INFORMATION
CAT#
SIZE
Poly(A)Purist™ Kit
1916
6 isolations;
each from up to 2 mg total RNA
Micro Poly(A)Purist™ Kit
1919
20 isolations;
each from up to 400 µg RNA
or 50 mg tissue or cells
Poly(A)Purist™ MAG Kit
1922
Magnetic bead-based isolation
from up to 8 mg total RNA
Poly(A)Purist™ MAG-96 Automated Kit
1813
384 purifications
Single Place Magnetic Stand
10026
1 each
Magnetic Stand-96
10027
1 each
Oligo(dT) Cellulose
10020
1 each
Elution Tubes
12480
100 x 2 ml tubes
ORDER ONLINE ANYTIME AT WWW.AMBION.COM
Ambion, Inc.
U.S. 800-888-8804 • Canada 800-445-1161
U.S. tel +1-512-651-0200 • fax +1-512-651-0201
Ambion (Europe) Ltd
tel +44 (0)1480-373-020
fax +44 (0)1480-373-010
Ambion K.K. (Japan)
tel +81 (0)3-5638-2181
fax +81 (0)3-5638-2182
For a list of Ambion direct free
phone numbers and distributors
in your country, go to
www.ambion.com/contact
An Applied Biosystems Business