Clean-up for Aflatoxin (B1,B2,G1,G2)
and Zearalenone
mu_226_040706
MultiSep®226 AflaZon+ Syringe format
To shorten the time-consuming clean-up process for analysis of aflatoxin and zearalenone, Romer®
Labs has developed two one-step clean-up columns for this group of mycotoxins. The MultiSep®224
AflaZon column contains a combination of adsorbents that was designed for complex commodities.
When more purification is necessary due to the complexity of the matrix, MultiSep®226 AflaZon+
containing a double portion of these same adsorbents might be preferred. Both columns are available
in two formats, the MultiSep® syringe format and the MycoSep® push-through format. Both formats
have been intensively studied and application briefs are available.
The following describes an example of a general procedure for MultiSep®226 AflaZon (For
detailed application briefs, see www.romerlabs.com):
Grind and weigh 25g of sample
Commodities studied:
• grains
Add 100mL 84+16 AcN+H2O,
blend for 3 minutes, or shake for
30 minutes
Filter supernatant
• silages
• feed
• food
Recovery:
>90%
Acidify 6ml extract with 60µl
acetic acid (in case of Zon)
Variability:
<5%
Apply 6mL of extract to
MultiSep®226 AflaZon+
Time for clean up:
60 seconds
Remove all liquid, evaporate 4ml
of the eluate to dryness
Redissolve in 400µl mobile phase
MultiSep® 226 AflaZon+ columns
Order No.:COCMU2226
Box of 25
MultiSep® 224 AflaZon columns
Order No.:COCMU2224
Box of 25
Analyze
Romer Labs, Inc. – America
Romer Labs Singapore Pte. Ltd.
Romer Labs Diagnostic GmbH
1301 Stylemaster Drive
Union, MO 63084-1156 USA
Tel: +1 636 583 8600
Fax: +1 636 583 2340
E-Mail: [email protected]
3791 Jalan Bukit Merah, #08-08
E-Centre@redhill, Singapore 159471
Tel: +65 6275 5432
Fax: +65 6275 5584
E-Mail: [email protected]
Industriestrasse 21
A-3130 Herzogenburg, Austria
Tel: +43 2782 803 0
Fax: +43 2782 330 550
E-Mail: [email protected]