1
CURRICULUM
VITAE
Tzyh-Chang (TC) Hwang
Date of birth: Feb. 14, 1957
Office address:
Membrane Biophysics Laboratory, DCRC Research Park
University of Missouri-Columbia, Columbia, MO 65211.
Telephone: 573-882-2181
Education:
1975 - 1982
1984 - 1986
1986 - 1990
Marital status: Married, two children.
Fax: 573-884-4232
e-mail: [email protected].
National Yang-Ming Medical School
M.D.
National Taiwan University
School of Medicine
M.S.
Department of Physiology, School of Medicine
The Johns Hopkins University
Ph.D.
Professional Experience:
1980 - 1982 Internship, Veteran General Hospital, Taipei, Taiwan R. O. C.
1982 - 1984 Surgeon Officer and Lieutenant Officer, R. O. C. Army
1984 - 1986 Teaching Assistant, Department of Physiology, National Yang-Ming Medical College
1990 - 1993 Postdoctoral Associate, Laboratory of Cardiac/Membrane Physiology
The Rockefeller University
1993 - 1994 Assistant Professor, Laboratory of Cardiac/Membrane Physiology
The Rockefeller University
1994 - 1999 Assistant Professor, Department of Physiology, University of Missouri-Columbia
1994 Research Investigator, Dalton Cardiovascular Research Center
1999 2004 Associate Professor, Department of Physiology, University of Missouri-Columbia
2001 – 2002 Visiting Professor, Department of Physiology and Biophysics, Cornell Medical School
2004 Professor, Department of Medical Pharmacology and Physiology, University of Missouri
2008 – 2009 Visiting Professor, Institute of Biophotonics, National Yang Ming University
Honors and Awards:
1984
Service Award by R. O. C. Army
1986 - 1990 Fellowship, Department of Physiology, The Johns Hopkins University
1989
FASEB Award, Cell and General Physiology, American Physiological Society
1990
Certificate of Merit, Young Investigator Day, The Johns Hopkins University
1991- 1994 Ella Fitzgerald Research Fellowship, New York Heart Association
1998
Excellence in Medical Student Education, MU Medical School.
1999
Excellence in Medical Student Education, MU Medical Student Affair Council.
1999
Nomination for Howard Hughes Assistant Investigatorship
2000
Professor-for-a-Day, School of Engineering, University of Missouri
2000
Paul Cranefield Award, Society of General Physiologists
2001
Excellence in Medical Student Education, MU Medical School
2001
Service Award, American Medical Student Association, University of Missouri
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2001
2007
2008
2009
Order of Socrates, MU Medical School
Honorary Visiting Professorship, Osaka Medical College, Japan
Visiting Professorship, Harbin Medical University, China
Outstanding Alumni Award, National Yang Ming University, Taiwan
National/International Service
1999
Cardiovascular A Study Section, NIH, ad hoc reviewer.
2000
General Medicine B Study Section, NIH, ad hoc member.
2003 - 2006 Molecular, Cellular and Developmental Neurosciences 3 Study Section, NIH, regular
member.
2003 - present Editorial Board, Journal of General Physiology
2006 - present Editorial Board, Biophysical Journal
2006 - 2007 Biophysics of Neural Systems Study Section, regular member.
2006
American Heart Association, “Cell Metabolism and Transport” Study Section.
2006 - present Consultant, Cystic Fibrosis Foundation Therapeutics
2007
Ad hoc member, Board of Scientific Council, NIH/NINDS.
2008
Ad hoc member, Special Emphasis Panel/Scientific Review Group ZRG1 RUS-C
2010 – present Member of College of CSR Reviewers, NIH.
Local Service
2004 – present Member, Graduate Educational Committee, Medical Pharmacology and Physiology
2004- present Member, Tenure and Promotion committee, Dalton Cardiovascular Research Center
Grant support (Total direct) as P.I. or Mentor:
1994 - 1996 Grant-in-aid, American Heart Association, Missouri Affiliate. $50,000
1995 - 1996 MU Research Board, $34,000
1995 - 1997 MU Molecular Biology Postdoc. Fellowship (Dr. Fei Wang), $47,000
1996 - 2000 NIH FIRST Award, $280,000
1996 - 1998 New Investigator Award, Cystic Fibrosis Foundation, $60,000
1996 - 1999 NIH/CFF RFA, $300,000
1997 - 1999 Postdoc. fellowship (Dr. Fei Wang), American Heart Association, Missouri Affiliate,
$55,000.
1997 - 1998 Trainingship (Shawn Zeltwanger), Cystic Fibrosis Foundation, $1,500
1998 - 1999 MU Molecular Biology Postdoc. Fellowship (Dr. Zhen Zhou), $23,500
1999 - 2001 Postdoc. fellowship (Dr. Zhen Zhou), American Heart Association, Missouri Affiliate,
$55,000.
1999 - 2003 NIHR01, NIDDK, “Molecular pathophysiology of cystic fibrosis”, $572,637
1999 - 2000 Cystic Fibrosis Foundation, $75,000
2000 - 2002 Postdoctoral fellowship (Dr. Allan Powe), Merck Foundation, $70,000
2000 - 2002 Grand-in-aid, American Heart Association (declined after one year of funding), $65,445
2001 - 2005 AHA Established Investigatorship (declined after three months of funding), $300,000
2000 - 2004 NIHR01, NHLBI, “Gating of CFTR chloride channels by ATP hydrolysis”, $700,000.
2000 - 2001 Trainingship (Lesley Nichols), Cystic Fibrosis Foundation, $1,500
2001 - 2004 Postdoc. fellowship (Dr. Zhen Zhou), Cystic Fibrosis Foundation, $100,000.
2002 - 2007 NIHK25 (Dr. Xiaoqin Zou), $500,000.
2003 - 2006 NIH NRSA Award (Dr. Silvia Bompadre), $150,348.
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2003 - 2005
2003 – 2007
2005 – 2006
2006 - 2007
2006 – 2010
2006 – 2009
2006 – 2009
2008 – 2013
2009 – 2011
AHA Postdoctoral fellowship (Dr. Tomohiko Ai), $80,685.
NIHR01, NIDDK, “Molecular pathophysiology of cystic fibrosis”, $640,000
Cystic Fibrosis Foundation, $75,000
NIHS10, “Confocal equipment for biomedical and nanomedicine research”, $402,030
NIHR01 NHLB, “Gating of CFTR chloride channels by ATP hydrolysis”,
$933,056
NIHK01 (Dr. Silvia Bompadre), NIDDK, “Molecular physiology and pharmacology of
CFTR chloride channels” $300,000
CFFT, “Structure-guided physiological screening of F508 CFTR correctors”.
$1,349,781
NIHR01, NIDDK, “Molecular pathophysiology of cystic fibrosis”, $1,014,155
CFF Postdoctoral Fellowship (Dr. Zoia Kopeikin), “On the mechanism of CFTR
inhibition by CFTRinh-172.” $86,100
Grant support (Total direct) as Co-P.I.:
1997 - 1998 Clinical Research Committee Award (P.I. Dr. Peter Konig), Cystic Fibrosis Foundation,
“Effects of genistein on F508 CFTR”, $60,262, 5% effort.
1997 - 2001 NIHR01 (P.I. Dr. Mark Milanick), “Cytosolic modulation of plasma membrane ion
transport”, $696,039, 5% effort.
1995 - 2000 NIH Training Grant (P.I. Dr. Virginia Huxley), “Cardiovascular and renal
physiology, pharmacology and biochemistry”, $704,666, 5% effort.
1999 - 2001 Research Grant (P.I. Dr. Fei Wang), Cystic Fibrosis Foundation, $100,350.
1999 - 2001 Research Grant (P.I. Dr. Layla Al-Nakkash), Cystic Fibrosis Foundation, $88,350.
2001 - 2003 NIHC06 (P.I.: Dr. Edward Blaine), "Conversion of Shell Space at the Dalton
Cardiovascular Research Center", $1,036,000.
2002 - 2005 NIH training grant (P.I. Dr. Virginia Huxley), “Cardiovascular & Renal Physiology,
Pharmacology & Biochemistry”, $648,270, 3% effort.
2003 - 2006 NIHR01 (P.I. Dr. Mark Milanick), “Cytosolic modulation of plasma membrane ion
transport”, $615,000, 5% effort.
2004 – 2005 NIH (PI. Dr. Kevin Gillis), Nanomedicine Center Concept Development Award, Phase I,
$36,000.
2006 – 2008 Research Grant (P.I. Dr. Zhen Zhou), Cystic Fibrosis Foundation, $80,000
2006 – 2008 Research Grant (P.I. Dr. Silvia Bompadre), Cystic Fibrosis Foundation, $160,000
2007 – 2009 NIHR21 (P.I. Dr. Dongsheng Duan), “Novel hybrid AAV vectors for cystic fibrosis gene
therapy”, $254,277, 5% effort.
Graduate students supervised:
1995 - 1998
Shawn Zeltwanger, Ph.D. (Physiology)
2003 - 2006
Jeong Han Cho (Bioengineering)
2005 - 2009
Xiaohui Wang (MPP)
2007 Ming-Feng Tsai (MPP)
2007 Yonghong Bai (Bioengineering)
2009 Kang-Yang Jih (MPP)
Postdoctoral fellow:
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1995 - 1999 Fei Wang (Rockefeller University, New York)
1996 - 1999 Layla Al-Nakkash (University of Newcastle-Upon-Tyne, England)
1997 - 1998 Guo-Tang Wang (Peiking Medical University, Peiking)
1998 - 2002 Allan Powe (Rockefeller University, New York)
1998 - 2005 Zhen Zhou (University of Michigan, Ann Arbor)
2000 - 2006 Silvia Bompadre (University of Washington, Seattle)
2001 - 2003 Tomohiko Ai (Kyoto University, Japan)
2006 - 2008 Hiroyasu Shimizu (Osaka Medical College)
2006 - 2009 Hao-Yang Liu (Wuhan University, China)
2007 - present Zoia Kopeikin (University of Missouri-Columbia)
Memberships:
Biophysical Society
Society of General Physiologist
American Physiological Society
Invited Speaker:
1992 Department of Pharmacology, Columbia University, School of Medicine.
6th Annual North American Cystic Fibrosis Conference.
1993 Department of Physiology, Cornell Medical School.
Cystic Fibrosis Research Group, The Johns Hopkins Medical School.
Department of Physiology, Oxford University, United Kingdom.
XXXll Congress of the International Union of Physiological Sciences
Max Planck Institute for Biophysics, Frankfurt, Germany.
Department of Physiology, University of Cologne, Cologne, Germany.
1994 International Society for Heart Research, London, Ontario, Canada.
48th Annual Symposium, Society of General Physiologists
1995 Gordon Research Conference, New Hampshire
Department of Pharmacology, University of Missouri-Columbia
1996 Williamsburg Meeting, Cystic Fibrosis Foundation
10th Annual North American Cystic Fibrosis Conference
Department of Molecular and Cellular Physiology, Yale University.
1997 Department of Veterinary Biomedical Sciences, University of Missouri-Columbia
Department of Physiology, Kyoto University, Japan
Department of Physiology, Tzu-Chi Medical College, Taiwan
Department of Physiology, National Chung-Kung University, Taiwan
Department of Physiology, National Yang-Ming Medical University, Taiwan
Alfred duPond Institute, Delaware
Center for Human Gene Therapy, University of Pennsylvania, Philadelphia
Williamsburg Meeting, Cystic Fibrosis Foundation
Department of Molecular Biophysics and Physiology, Rush Medical Center, Chicago
11th Annual North American Cystic Fibrosis Conference
Genzyme Corp. Hamingham, MA.
1998 Williamsburg Meeting, Cystic Fibrosis Foundation
12th Annual North American Cystic Fibrosis Conference
1999 Department of Physiology, National Taiwan University
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2000
2001
2002
2003
2004
2005
2007
2008
2009
Department of Physiology, National Yang-Ming Medical University, Taiwan
Department of Biological and Agricultural Engineering, University of Missouri-Columbia
Department of Physics, University of Missouri-Columbia
Nutrition Group, University of Missouri-Columbia
Neurobiology Group, University of Missouri-Columbia
Department of Physiology, Emory University
Gordon Conference
Alfred duPond Institute, Delaware
Department of Physics, Wuhan University, China
Department of Physiology and Biophysics, University of Colorado
Osaka Medical College
Department of Physiology, Kyoto University
Williamsburg Meeting
Department of Physiology and Biophysics, Cornell Medical College
Department of Physiology, University of California, Davis
Department of Physiology, Robert Wood Johnson Medical School
Institute of Human Gene Therapy, University of Pennsylvania
Department of Physiology, Johns Hopkins Medical School
Department of Biological Engineering, University of Missouri-Columbia
Department of Physics, University of Missouri-Columbia
Gordon Conference
European Cystic Fibrosis Conference, Tomar, Portugal
Department of Physiology, Osaka Medical College, Japan
Osaka University of Pharmatheutical Science, Japan
Veteran General Hospital, Taichun, Taiwan
Department of Physiology, National Cheng-Kung University, Taiwan
Institute of Molecular Biology, National Chung-Cheng University, Taiwan
North American Cystic Fibrosis Conference
Gordon Research Conference
North American Cystic Fibrosis Conference
National Yang-Ming Medical University, Institute of Neuroscience
Special Satellite Meeting on Anion Transport for the Society of Physiologists, United Kingdom
Department of Pharmacology, National Yang-Ming Medical University
Department of Physiology, Osaka Medical College, Japan
Department of Internal Medicine, Taichun Veteran General Hospital
Osaka University of Pharmatheutical Science, Japan
Department of Anesthesiology, Washington University
Department of Physiology, University of Illinois, Urbana Champaign
Life Science Institute, Northeast Normal University, Changchun, China
Symposium speaker, Joint meeting of the Physiological Society, the Chinese Association for
Physiological Societies, and the American, Australian and Canadian Physiological Societies,
Beijing China.
Department of Physiology, Chinese University of Hong Kong
Harbin Medical University
Department of Pharmacology, Keio University, Japan
Department of Biology, Hong Kong University of Science and Technology
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2010
Institute of Biomedical Sciences, Academia Sinica, Taiwan, ROC
European Cystic Fibrosis Conference, Portugal
Keynote speaker, 14th Annual Conference of the Biophysical Society of ROC, Taiwan
Keynote speaker, Annual Conference for Collaborative Projects between National Taiwan
University Hospital and Veteran General Hospital, Taipei, Taiwan
Kyoto Prefectural University of Medicine
IUPS2009, Kyoto, Japan
Special Satellite Meeting on Anion Transport for IUPS, Okazaki, Japan
Fu-Jen University, School of Medicine, Taipei, Taiwan
Department of Physiology, Xian JiaoTong University, School of Medicine, Xian, China
Department of Physiology and Pharmacology, Chang Gung University, Taiwan
Department of Electrical Engineering, National Taiwan University
Bethune Second Hospital, Jilin University, China
Department of Cell Physiology and Molecular Biophysics, Texas Tech University
Meeting Chair:
1994 Biophysical Meeting
1997 11th Annual North American Cystic Fibrosis Conference
2003 Society of General Physiologists Meeting, Woods Hole, Massachusetts
2005 Special Satellite Meeting on Anion Transport for the Society of Physiologists, Bristol, United
Kingdom
2006 Williamsburg Conference, Cystic Fibrosis Foundation
2007 FASEB Meeting, Washington DC
2009 Special Satellite Meeting on Anion Transport for IUPS, Okasaki, Japan
Journal Reviewer:
American Journal of Physiology (cell physiology), American Journal of Physiology (heart and
circulation), Journal of General Physiology, Neuron, Journal of Membrane Biology, Journal of
Biological Chemistry, Journal of Pharmacology and Experimental Therapeutics, Journal of Molecular
and Cellular Cardiology, Canadian Journal of Physiology and Pharmacology, Biophysical Journal,
Journal of Physiology, Brain Research, American Journal of Physiology (Lung, Cell and Mole),
Proceedings of National Academy of Sciences, USA, FEBS Lett., Nature, Biochemical Journal,
Bioorganic and Medicinal Chemistry, Nature Structural Biology, PLoSONE, Biosci Report..
Grant Reviewer: Cystic Fibrosis Trust (United Kingdom), Cystic Fibrosis Foundation, Cystic Fibrosis
Research Inc., MU Research Board, Veteran Affairs, NIH (CVA, ad hoc reviewer), NIH (GMB, ad hoc
member), NIH (MCDN3, regular member), Philips Morris External Research Program, Italian Telethon
Foundation, American Heart Association (National Center), Dutch Digestive Foundation, Wellcome
Trust (United Kingdom), National Science Foundation, Academia Sinica (ROC).
Publications:
PAPERS:
1. Tong J. Chen, Tzyh-Chang Hwang, Chiun Chien Huang, Guey Mei Jow, and John Yuh-lin Yu. (1989)
Deciduomal estrogen and progesterone receptors in unilateral pregnant hamsters. Endocrinology ,
125:302-307.
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2. Tzyh-Chang Hwang, Luo Lu, Pamela Zeitlin, Dieter Gruenert, Richard Huganir and William B.
Guggino.(1989). Chloride channels in cystic fibrosis: Lack of activation by protein kinase C and cAMPdependent protein kinase. Science, Wash. DC. 244:1351-1353.
3. Luo Lu, Chahrzad Mintrose-Refizadeh, Tzyh-Chang Hwang and William B. Guggino. (1990) A
delayed rectifier potassium current in Xenopus oocyte. Biophys. J. 57:1117-1123.
4. Tzyh-Chang Hwang, Sandra E. Guggino and William B. Guggino. (1990). Direct modulation of
secretory chloride channels by arachidonic and other Cis-unsaturated fatty acids. Proc. Natl. Acad. Sci.
USA. 87:5706-5709.
5. Tzyh-Chang Hwang, Minoru Horie, Angus C. Nairn and David C. Gadsby. (1992) Roles of GTPbinding proteins in the regulation of mammalian cardiac chloride conductance. J. Gen. Physiol. 99:
465-489.
6. Minoru Horie, Tzyh-Chang Hwang and David C. Gadsby. (1992). Pipette GTP is essential for receptormediated regulation of Cl current in dialysed myocytes from guinea-pig ventricle. J. Physiol. 455:235245.
7. Georg Nagel, Tzyh-Chang Hwang, Kent Nastiuk, Angus C. Nairn And David C. Gadsby. (1992). The
protein kinase A-regulated cardiac Cl channel resembles CFTR (Cystic Fibrosis Transmembrane
Conductance Regulator). Nature. 360:81-84.
8. Tzyh-Chang Hwang, Marie E. Egan and William B. Guggino. (1992). Kinetic interaction of fatty acids
with outwardly rectifying chloride channels. Cell. Physiol. Biochem. 2:308-315.
9. Tzyh-Chang Hwang, Minoru Horie and David C. Gadsby. (1993) Functionally distinct phospho-forms
underlie incremental activation of PKA-regulated Cl conductance in mammalian heart. J. Gen. Physiol.
101:629-650.
10. David C. Gadsby, Tzyh-Chang Hwang, Minoru Horie, Georg Nagel and Angus C. Nairn. (1993)
Cardiac chloride channels: incremental regulation by phosphorylation/dephosphorylation. Ann. N. Y.
Acad. Sci. 707:259-274.
11. Tzyh-Chang Hwang, Georg Nagel, Angus C. Nairn and David. C. Gadsby. (1994). Regulation of the
gating of CFTR Cl channels by phosphorylation and ATP hydrolysis. Proc. Natl. Acad. Sci. USA.
91:4698-4702.
12. Thomas Baukrowitz, Tzyh-Chang Hwang, Angus C. Nairn and David. C. Gadsby. (1994). Coupling
of CFTR channel gating to an ATP hydrolysis cycle. Neuron. 12:473-482.
13. David C. Gadsby, Tzyh-Chang Hwang, Thomas Baukrowitz, Georg Nagel, Minoru Horie and Angus C.
Nairn. (1994). Regulation of CFTR channel gating. Jap. J. Physiol. 44:S183-S192.
14. Iris Yang, Tzu-Hurng Cheng, Fei Wang, Elmer Price and Tzyh-Chang Hwang. (1997). Modulation of
CFTR chloride channels by calyculin A and genistein. Am. J. Physiol. 272:C142-C155.
15. Tzyh-Chang Hwang, Fei Wang, Iris Yang, and William W. Reenstra. (1997). Genistein potentiates
wild-type and F508 CFTR channel. Am. J. Physiol. 273:C988-C998.
16. Jumming Zhong, Tzyh-Chang Hwang, Richard Adams, and Leona Rubin. (1997). Reduced L-type
calcium current in ventricular myocytes from endotoxemic guinea pigs. Am. J. Physiol. 273:H2312H2324.
17. Fei Wang, Shawn Zeltwanger, Iris Yang, Angus Nairn, and Tzyh-Chang Hwang. (1998). Action of
genistein on CFTR gating: evidence of two binding sites with opposite effects. J. Gen. Physiol. 111:477490.
18. Layla Al-Nakkash, and Tzyh-Chang Hwang. (1999). Activation of wild-type and F508-CFTR by
phosphodiesterase inhibitors. Pflügers Arch. 437:553-561.
19. Shawn Zeltwanger, Fei Wang, Guo-Tang Wang, Kevin Gillis, and Tzyh-Chang Hwang. (1999). Gating
of CFTR by nucleoside triphosphates: quantitative analysis of a cyclic gating scheme. J. Gen. Physiol.
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113:541-554.
20. Fei Wang, Shawn Zeltwanger, Shenghui Hu, and Tzyh-Chang Hwang. (2000). Deletion of
phenylalanine 508 causes attenuated phosphorylation-dependent activation of CFTR chloride channels.
J. Physiol. 524:637-648..
21. Layla Al-Nakkash, Shenghui Hu, Min Li, and Tzyh-Chang Hwang. (2001). A common mechanism for
CFTR activation by benzimidazolone analogs and genistein. J. Exp. Pharm. Ther. 296:464-472.
22. Zhen Zhou, Shenghui Hu, and Tzyh-Chang Hwang. (2001). Voltage-dependent flickery blockade
of an open CFTR channel. J. Physiol. 532:435-448.
23. Peng Chen, Tzyh-Chang Hwang, and Kevin Gillis. (2001).The relationship between cAMP, Ca and
transport of CFTR to the plasma membrane. J. Gen. Physiol.118:135-144.
24. Allan Powe, Layla AL-Nakkash, Min Li, and Tzyh-Chang Hwang. (2002). Mutations of the Walker
A lysine 464 in CFTR reveal functional interaction between its two nucleotide binding domains. J.
Physiol. 539:333-346.
25. Zhen Zhou, Shenghui Hu, and Tzyh-Chang Hwang (2002). Probing an open CFTR pore with
organic anion blockers. J. Gen. Physiol. 120:647-662.
26. Tzyh-Chang Hwang, Roger Koeppe, II. and Olaf Andersen (2003). Genistein can modulate channel
function by a phosphorylation-independent mechanism: Importance of bilayer mechanics and
hydrophobic mismatch. Biochemistry 42:13646-13658.
27. Tomohiko Ai, Silvia Bompadre, Xiaohui Wang, Shenghui Hu, Min Li, and Tzyh-Chang Hwang
(2004). Capsaicin potentiates wild-type and mutant CFTR chloride channel currents. Molecular
Pharmacology 65:1415-1426.
28. Lane L. Clarke, Lara R. Gawenis, Tzyh-Chang Hwang, Nancy M. Walker, Darren B. Gruis and
Elmer M. Price (2004). A Domain Mimic Increases {Delta}F508 CFTR Trafficking and Restores
cAMP-Stimulated Anion Secretion in Cystic Fibrosis Epithelia. Am. J. Physiol. 287:C192-199.
29. Tomohiko Ai, Silvia Bompadre, Yoshiro Sohma, Min Li and Tzyh-Chang Hwang (2004). Direct
effects of 9-anthracene compounds on CFTR gating. Pflügers Arch. 449:88-95.
30. Silvia G. Bompadre, Tomohiko Ai, Jeong Han Cho, Xiaohui Wang, Yoshiro Sohma, Min Li, and TzyhChang Hwang. (2005). CFTR gating I: Characterization of the ATP-dependent gating of a
phosphorylation-independent CFTR channel (R-CFTR). J. Gen. Physiol. 125:361-375.
31. Silvia G. Bompadre, Jeong Han Cho, Xiaohui Wang, Xiaoqin Zou, Yoshiro Sohma, Min Li, and
Tzyh-Chang Hwang. (2005). CFTR gating II: Effects of nucleotide binding on the stability of open
states. J. Gen. Physiol. 125:377-394.
32. Zhen Zhou, Xiaohui Wang, Min Li, Yoshiro Sohma, Xiaoqin Zou, and Tzyh-Chang Hwang.
(2005). High affinity ATP/ADP analogs as new tools for studying CFTR gating. J. Physiol. 569:447457.
33. Zhen Zhou, Xiaohui Wang, Haoyang Liu, Xiaoqin Zou, Min Li, and Tzyh-Chang Hwang. (2006).
The two ATP binding sites of Cystic Fibrosis Transmembrane conductance Regulator (CFTR) play
distinct roles in gating kinetics and energetics. J. Gen. Physiol. 128:413-422.
34. Silvia G. Bompadre, Yoshiro Sohma, Min Li, and Tzyh-Chang Hwang. (2007). G551D and
G1349D, two CF-associated mutations, exhibit distinct gating defects. J. Gen. Physiol. 129(4):28598.
35. Yen-Chin Chao, Shwu-Fen Chang, Shao-Chun Lu, Tzyh-Chang Hwang, Wei-Hsien Hsieh and
Jiahorng Liaw. (2007). Ethanol enhanced in vivo gene delivery with non-ionic polymeric micelles
inhalation. J. Control Release 118(1):105-17.
36. Silvia Bompadre, Min Li, and Tzyh-Chang Hwang. (2008). P-ATP potentiates G551D-CFTR by
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binding to the hydrolysis-incompetent site. J. Biol. Chem. 283:5364-5369.
37. K. Mio, T. Ogura, M. Mio, H. Shimizu, T. –C. Hwang, C. Sato, and Y. Sohma. (2008). Threedimensional Reconstruction of Human Cystic Fibrosis Transmembrane Conductance Regulator
Chloride Channel Revealed an Ellipsoidal Structure with Orifices beneath the Putative
Transmembrane Domain. J. Biol. Chem. 283(44):30300-30310.
38. Xiaohui Wang, Silvia Bompadre, Min Li and Tzyh-Chang Hwang (2009). Mutations at the
signature sequence of CFTR create a cadmium-gated chloride channel. J. Gen. Physiol. 133:67-77.
39. Sheng-You Huang, Diana Bolser, Hao-Yang Liu, Tzyh-Chang Hwang and Xiaoqin Zou. (2009).
Molecular modeling of the heterodimer of human CFTR’s nucleotide-binding domains using a
protein-protein docking approach. J. Mol. Graph. Model, 27:822-828.
40. Mingfeng Tsai, Hiroyasu Shimizu, Yoshiro Sohma, Min Li, and Tzyh-Chang Hwang (2009). Statedependent modulation of CFTR gating by pyrophosphate. J. Gen. Physiol. 133:405-419.
41. Ming-Feng Tsai, Min Li, and Tzyh-Chang Hwang. (2010). Stable ATP binding mediated by a partial
NBD dimer of the CFTR chloride channel. J. Gen. Physiol. 135:399-414.
42. Haruna Miki, Zhen Zhou, Min Li, Tzyh-Chang Hwang, and Silvia Bompadre. (2010). Potentiation
of disease-associated CFTR mutants by hydrolysable ATP analogs. J. Biol. Chem. In press.
43. Ming-Feng Tsai, Kang-Yang Jih, Min Li, and Tzyh-Chang Hwang. (2010). Optimization of the
composite site 1 rectifies the functional defects of cystic fibrosis related mutant CFTR channels.
Submitted.
44. Yonghong Bai, Min Li, and Tzyh-Chang Hwang. (2010). Dual roles of the sixth transmembrane
segment of the CFTR chloride channel in gating and permeation. Submitted.
REVIEWS and BOOK CHAPTERS:
1. Tzyh-Chang Hwang and David C, Gadsby (1994). Chloride ion channels in mammalian heart.
Current Topics in Membrane and Transports. 42:317-346.
2. David C, Gadsby, Georg Nagel and Tzyh-Chang Hwang (1995). CFTR chloride channels in
mammalian heart. Ann. Rev. Physiol. 57:387-416.
3. Tzyh-Chang Hwang and David Sheppard (1999). Molecular pharmacology of the CFTR chloride
channel. TIPS, 11:448-453.
4. Xiaoqin Zou and Tzyh-Chang Hwang. (2001). ATP hydrolysis-coupled gating of CFTR chloride
channels: structure and function. Biochemistry, 40:5579-5586.
5. Allan. C. Powe, Zhen Zhou, Tzyh-Chang Hwang, and G. Nagel. (2002). Quantitative analysis of ATPdependent CFTR gating. Methods in Molecular Medicine, 70-67-98.
6. Sheppard DN, Gray MA, Gong X, Sohma Y, Kogan I, Benos DJ, Scott-Ward TS, Chen JH, Li H, Cai Z,
Gupta J, Li C, Ramjeesingh M, Berdiev BK, Ismailov II, Bear CE, Hwang TC, Linsdell P, and Hug MJ.
(2004) The patch-clamp and planar lipid bilayer techniques: powerful and versatile tools to investigate
the CFTR Cl- channel. J. Cyst. Fibros. Suppl 2: 101-108
7. Zhen Zhou and Tzyh-Chang Hwang. (2007). Gating of Cystic Fibrosis Transmembrane conductance
Regulator (CFTR) chloride channels. Advan. Mole. Cell. Biol. 38:145-180.
8. Silvia, Bompadre and Tzyh-Chang Hwang. (2007). CFTR: a chloride channel gated by ATP binding
and hydrolysis. Acta Physiolgica Sinica 59(4):431-42.
9. Tsung-Yu Chen and Tzyh-Chang Hwang. (2008). CLC-0 and CFTR: two chloride channels evolved
from transporters. Physiol. Rev. 88:351-387.
10. Tzyh-Chang Hwang and David Sheppard. (2009). Gating of the CFTR chloride channel by ATP-driven
nucleotide-binding domain dimerisation. J. Physiol. 587:2151-2161.
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11. Tsung-Yu Chen, Ming-Feng Tsai, and Tzyh-Chang Hwang. (2010). Structures and mechanisms of
chloride channels. Submitted.
12. Zhiwei Cai, Yoshiro Sohma, Silvia Bompadre, David, N. Sheppard, and Tzyh-Chang Hwang. (2010).
Application of high-resolution single-channel recording to functional studies of cystic fibrosis mutants
and small molecule CFTR potentiators. Submitted.
ABSTRACTS:
1. Tong J. Chen, Tzyh-Chang Hwang, S. M. Chuang. Maintenance of Deciduomal and Decidual
Responses in Pregnant Hamsters. The First Joint Annual Conference of Biomedical Science 1986,
Taiwan, R.O.C. p92.
2. Tzyh-Chang Hwang, Luo Lu, Pamela Zeitlin, Dieter Gruenert, Richard Huganir and William B.
Guggino. (1989) Activation of Epithelial Cl Channel by Protein Kinase C and cAMP-dependent Protein
Kinase. FASEB J. 3:A1149.
3. Tzyh-Chang Hwang, Luo Lu, Pamela Zeitlin, Richard Huganir and William Guggino. (1989) Chloride
Channels in Cystic Fibrosis: Lack of Activation by Protein Kinase C and cAMP-dependent Protein
Kinase. Ped. Pulmonol. Suppl 4):116.
4. Tzyh-Chang Hwang, Sandra E. Guggino and William B. Guggino. (1990) Arachidonic acid block of
epithelial Cl channels in tracheal airway cells. Biophys. J. 57, 88a.
5. Tzyh-Chang Hwang, Minoru Horie, and David C. Gadsby (1991). Persistent activation of chloride
current by GTPS in guinea pig ventricular myocytes. Biophys. J. 59, 7a.
6. Minoru Horie, Tzyh-Chang Hwang, and David C. Gadsby (1991). Okadaic acid modulates chloride
current activated by cAMP-dependent protein kinase in guinea pig ventricular myocytes. Biophys. J. 59,
90a.
7. Minoru Horie, Tzyh-Chang. Hwang, and David C. Gadsby. (l991) Intra-pipette GTP prevents the rapid
rundown of ß adrenoceptor-mediated activation of Cl current in isolated guinea-pig ventricular myocytes
J. Physiol., 446, 153P.
8. Minoru Horie, Tzyh-Chang Hwang, and David C. Gadsby. (l992) Forskolin activation of PKAregulated chloride current in guinea pig ventricular myocytes. Biophys. J . 61, A396.
9. Tzyh-Chang Hwang, M. Horie, A. G. Dousmanis, and D. C. Gadsby. (l992). Interactive modulation of
PKA-regulated chloride current by forskolin, Gs, and Gi in guinea pig ventricular myocytes. Biophys. J.
61, A395.
10. Georg A. Nagel, Tzyh-Chang Hwang, Allan C. Powe, Angus C. Nairn, and David C. Gadsby. (l992)
Chloride channels activated by protein kinase A in giant patches excised from guinea pig ventricular
myocytes. Biophys. J. 61, A146.
11. Tzyh-Chang Hwang, M. Horie, A. G. Dousmanis, and D. C. Gadsby. (l992). Regulation of PKAactivated Cl conductance in guinea pig ventricular myocytes: whole-cell studies. J. Gen. Physiol.
100:69a.
12. Georg A. Nagel, Tzyh-Chang Hwang, Angus C. Nairn, and David C. Gadsby. (l992) Regulation of
PKA-activated Cl conductance in guinea pig ventricular myocytes: single channel studies. J. Gen.
Physiol. 100:70a.
13. Tzyh-Chang Hwang, Georg A. Nagel, Angus C. Nairn, and David C. Gadsby. (1993).
Dephosphorylation of cardiac CFTR Cl channels requires multiple protein phosphatases. Biophys. J.
46:A343.
14. Tzyh-Chang Hwang, Georg A. Nagel, Angus C. Nairn, and David C. Gadsby. (1994). Differential
regulation of nucleotide binding sites in CFTR by PKA phosphorylation. Biophys. J. 66:A141
15. Georg A. Nagel, Tzyh-Chang Hwang, Angus C. Nairn, and David C. Gadsby. (1994). AMP-PNP
delays the closing of CFTR Cl channels opened by ATP. Biophys. J. 66:A141.
11
16. Thomas Baukrowitz, Tzyh-Chang Hwang, Angus C. Nairn, and David C. Gadsby. (1994). Opening of
CFTR Cl channels is coupled to ATP hydrolysis. Biophys. J. 66:A141.
17. David C. Gadsby, Tzyh-Chang Hwang, Georg A. Nagel, Minoru, Horie, and Angus C. Nairn. (1994).
Regulation of CFTR Cl channel gating by PKA phosphorylation and ATP hydrolysis. Biophys. J. 66:A2.
18. Tzyh-Chang Hwang and Elmer Price. (1995). Activation of CFTR chloride channels by forskolin and
genistein. Biophys. J. 68:A241.
19. Tzu-Hurng Cheng, Chun-Hui Yang, Elmer Price and Tzyh-Chang Hwang. (1995). Expression and
regulation of CFTR chloride channels in Hi-5 insect cells. Ped. Pulmon. S12:p196.
20. Fei Wang, Chun-Hui Yang, Elmer Price and Tzyh-Chang Hwang. (1996). Stable sub-conductance
states of CFTR chloride channels. Biophys. J. 70:A367.
21. Chun-Hui Yang, Fei Wang, Elmer Price and Tzyh-Chang Hwang. (1996). Modulation of CFTR
chloride channels by tyrosine kinase inhibitors. Biophys. J. 70:A412.
22. Tzyh-Chang Hwang. (1996). Genistein rescues channel function of the F508 CFTR. Ped. Pulmon.
S13:p257.
23. Chun-Hui Yang, Shawn Zeltwanger, Fei Wang, and Tzyh-Chang Hwang. (1996). Pharmacological
modulation of wild-type and F508 CFTR by genistein and CPX. Ped. Pulmon. S13:p258.
24. Tzyh-Chang Hwang and Layla Al-Nakkash. (1997). Defective phosphorylation activation of the
F508 CFTR. Biophys. J. 72:A113.
25. Fei Wang and Tzyh-Chang Hwang. (1997). Effects of genistein on CFTR chloride channel gating.
Biophys. J. 72:A113.
26. Layla Al-Nakkash. and Tzyh-Chang Hwang (1998). Phosphodiesterase inhibitors activate CFTR in the
human airway epithelial cell line, Calu-3. Biophys. J. 74:A343.
27. Fei Wang, Shawn Zeltwanger and Tzyh-Chang Hwang. (1998). Phosphorylation-dependent
activation of CFTR: kinetic evidence for multiple phosphorylation sites. Biophys. J. 74:A344.
28. Shawn Zeltwanger, Fei Wang and Tzyh-Chang Hwang. (1998). Gating of CFTR chloride channels
by nucleoside triphosphates. Biophys. J. 74:A395.
29. Shawn Zeltwanger, Fei Wang, Guo-Tang Wang, Kevin Gillis, and Tzyh-Chang Hwang. (1999). A
cyclic gating scheme of CFTR chloride channels. Biophys. J. 74:A395.
30. Allan Powe and Tzyh-Chang Hwang. (2000). Differential phosphorylation of CFTR modulates
NBD2 function. Biophys. J. 78:351A.
31. Peng Chen, Tzyh-Chang Hwang, and Kevin Gillis. (2000). Does cyclic AMP lead to transport of
CFTR to the plasma membrane? (2000). Biophys. J. 78:318A.
32. Bompadre, S., S. Hu, and T. -C. Hwang. (2001). Characterization of a proton-activated chloride
channel. Biophys. J. 80:468A.
33. Powe, A.C., Jr., L. Al-Nakkash, and T. -C. Hwang. (2001). The role of CFTR’s Walker-A lysine 464 in
ATP-dependent gating. Biophys. J. 80:470A.
34. Hwang, T. -C. and O. S. Andersen. (2002). Effects of genistein on gramicidin A channels in lipid
bilayers. Biophys. J.
35. Z. Zhou, S. Hu, T. -C. Hwang (2003). Probing An Open CFTR Pore with Organic Anion Blockers.
Biophys. J. 84:401.
36. T. Ai, Y. Sohma, T. -C. Hwang. (2003). Effects of anthracene-9-carboxylate on CFTR gating
Biophys. J. 84:401.
37. Y. Sohma, T. Ai, T. -C. Hwang. (2003). Voltage-dependent blockade of CFTR by anthracene-9carboxylic acid. Biophys. J. 84:485.
12
38. Y. Sohma, Z. Zhou, K. Yerrkondreddygari, T. –C. Hwang. (2004). Evidence for a superficial external
binding site in CFTR channel pore. Biophys. J. 86:332a.
39. J. Cho, M. Li, T. –C. Hwang. (2005), Differential gatingof a ligand-gated CFTR mutant by ATP and
AMP-PNP. Biophys. J. 88:30a.
40. Z. Zhou, X. Wang, H. Liu, M. Li, X. Zou, T. –C. Hwang. (2005). Distinct functions of individual
nucleotide binding domains in CFTR gating revealed by mutations in ATP binding pockets. Biophys. J.
88:546a.
41. S. Bompadre, J. Cho, X. Wang, Y. Sohma, M. Li, T. –C. Hwang. (2005). CFTR gating: nucleotide
binding and the stability of the open state. Biophys. J. 88:615a.
42. Z. Zhou, X. Wang, M. Li, Y. Sohma, X. Zou, T. –C. Hwang. (2006). High-affinity ATP/ADP analogues
as new tools to study CFTR gating. Biophys. J. 90:310a.