PRODUCT BULLETIN
Custom TaqMan® Array Cards
Custom TaqMan® Array Cards
• Validate microarray hits quickly and economically
• Standardize screening of gene sets across multiple samples and laboratories
• Create the perfect TaqMan® Array Card by incorporating your own
specific design needs
• Load 384 wells in typically less than
five minutes without liquid-handling
robots or multichannel pipettors The Custom TaqMan® Array Card is a
384-well microfluidic card that enables
you to perform 384 simultaneous realtime PCR reactions without the need for
liquid-handling robots or multi-channel
pipettors to load samples. This highly flexible
card enables 1 to 8 samples to be run
in parallel against 12 to 384 TaqMan®
Gene Expression Assay targets (including
a manufacturing control). The assays
are preloaded into each well of the
card and are ready for use immediately
upon delivery. Choose from over 50,000
inventoried TaqMan® Gene Expression
Assays designed for human, mouse, and
rat genes to be loaded onto a Custom
TaqMan® Array Card (Figure 1). For added
flexibility, you can contact your local Life
Technologies sales representative to order
cards loaded with Predesigned made-toorder or Custom TaqMan® Gene Expression
Assays. The Custom TaqMan® Array Card
is designed for use on the state-of-the-art
QuantStudio™ 12K Flex, or ViiA™ 7 Real-Time
PCR Systems when equipped with TaqMan®
Array Card blocks.
Ultimate microarray validation tool
TaqMan® Array Cards are exactly the right
tool for validating the tens or hundreds of hits
generated from microarrays because they can
be customized to include up to 384 of those
hits in one easy-to-use card. Using individual
TaqMan® Assays, or even SYBR® dye–based
assays, to look at 12, 48, or 96 targets can
quickly become unmanageable and expensive.
TaqMan® Array Cards enable researchers to
accomplish the validation necessary to arrive at
the right answer easily and affordably.
Ideal screening technology
TaqMan® Array Cards are ideal for screening
biomarkers and toxicology panels, and for
analyzing pathways, target classes, and
complete disease sets. Because TaqMan®
Array Cards do not require liquid-handling
robots for loading, you get standardized
results with low variability across multiple
users and laboratories. Plus, TaqMan® Gene
Expression Assays—the gold standard of
specificity and sensitivity in real-time
PCR—are preloaded onto the TaqMan® Array
Card, enabling reliable performance.
Figure 1. Custom TaqMan ® Array Cards can be
designed by researchers using any of over 50,000
inventoried TaqMan ® Gene Expression Assays—
the gold standard in real-time PCR specificity
and sensitivity.
Create the perfect card
You simply select TaqMan® Gene
Expression Assays and the optimal
Custom TaqMan® Array Card format for your
experiment, and we will deliver your TaqMan®
Array Card preloaded with your selected
assays. Choose 12 to 384 target assays
from our collection of inventoried TaqMan®
Gene Expression Assays covering human,
mouse, and rat genes. Ordering is easy with
the online Custom TaqMan® Array Card
configuration tool, which helps you find and
select genes and assays. Custom TaqMan®
Array Cards are available in 10 different
formats covering 12, 16, 24, 32, 48, 64, 96
(2 choices), 192, and 384 assays.
A
Designing a Custom TaqMan® Array Card
Customizing a TaqMan® Array card
through the Life Technologies website at
lifetechnologies.com/arraycards is simple.
Choose your array format and method for
choosing TaqMan® Gene Expression Assays,
select a quantity, and place your order—it
is as easy as that. Online selection of
TaqMan® Assays is simple: search by
using common keywords like Entrez gene
symbols, RefSeq accession numbers, or
UniGene IDs (Figure 2A). You can narrow
your search results using the Target Class
filters relevant to your research area
(Figure 2B). For example, if you want to
find TaqMan® Assays for caspase genes in
the area of apoptosis, select the “Apoptosis”
target class filter and enter “caspase” in
the Keyword Search box to return results
for caspase genes (Figure 2C).
B
C
Additionally, TaqMan® Gene Panels, which
are predefined gene panels of TaqMan®
Gene Expression Assays corresponding to
a biological pathway or target class, can be
used to simplify configuration of a Custom
TaqMan® Array Card. The TaqMan® Gene
Panels are accessible through the online
Custom TaqMan® Array Card configurator.
Figure 2. Customizing a TaqMan ® Array Card using our simple yet powerful search tool can be done quickly
online at lifetechnologies.com/arraycards.
®
Custom
TaqMan
Array Arrays
Cards
TaqMan
Custom
®
Custom TaqMan® Array Cards can be ordered in any of these ten format options.
TaqMan Custom Arrays can be ordered in any of these nine format options.
Format 12Format
(Cat. No.124342247)
(P/N 4342247)
11 unique assays + 1 mandatory control
11 unique assays + 1 mandatory control
8 unique samples
8 unique samples
Replicates
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Format 16Format
(Cat. No.164346798)
(P/N 4346798)
15 unique assays + 1 mandatory control
15 unique assays + 1 mandatory control
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8 unique samples
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Format 48Format
(Cat. No.484342253)
(P/N 4342253)
47 unique assays + 1 mandatory control
47 unique assays + 1 mandatory control
8 unique samples
8 unique samples
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Format 32Format
(Cat. No.324346799)
(P/N 4346799)
31 unique assays + 1 mandatory control
31 unique assays + 1 mandatory control
4 unique samples
4 unique samples
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Format 24Format
(Cat. No.244342249)
(P/N 4342249)
23 unique assays + 1 mandatory control
23 unique assays + 1 mandatory control
8 unique samples
8 unique samples
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Format 64Format
(Cat. No.
644346800)
(P/N 4346800)
63 unique assays + 1 mandatory control
63 unique assays + 1 mandatory control
2 unique samples
2 unique samples
Replicates
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2
Format 96a
(Cat. No.
Format
96a4342259)
(P/N 4342259)
95 unique assays + 1 mandatory control
95 unique assays + 1 mandatory control
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33
41
49
57
2
9
17
25
33
41
49
57
2
9
17
25
33
41
49
57
2
9
17
25
33
41
49
57
2
9
17
25
33
41
49
57
3
10
18
26
34
42
50
58
3
10
18
26
34
42
50
58
3
10
18
26
34
42
50
58
3
10
18
26
34
42
50
58
3
10
18
26
34
42
50
58
3
10
18
26
34
42
50
58
11
19
27
35
43
51
59
11
19
27
35
43
51
59
11
19
27
35
43
51
59
4
12
20
28
36
44
52
60
4
12
20
28
36
44
52
60
1
2
3
4
5
6
7
8
1
24
48
72
1
24
48
72
1
24
48
72
1
24
48
72
2
25
49
73
2
25
49
73
2
25
49
73
2
25
49
73
3
26
50
74
3
26
50
74
3
26
50
74
3
26
50
74
4
27
51
75
4
27
51
75
4
27
51
75
4
27
51
75
5
28
52
76
5
28
52
76
5
28
52
76
5
28
52
76
6
29
53
77
6
29
53
77
6
29
53
77
6
29
53
77
7
30
54
78
7
30
54
78
7
30
54
78
7
30
54
78
1
2
3
4
5
6
1
1
24
24
48
48
72
72
1
1
24
24
48
48
72
72
2
2
25
25
49
49
73
73
2
2
25
25
49
49
73
73
3
3
26
26
50
50
74
74
3
3
26
26
50
50
74
74
4
4
27
27
51
51
75
75
4
4
27
27
51
51
75
75
5
5
28
28
52
52
76
76
5
5
28
28
52
52
76
76
1
2
3
4
1
1
24
24
48
48
72
72
96
96
120
120
144
144
168
168
2
2
25
25
49
49
73
73
97
97
121
121
145
145
169
169
3
3
26
26
50
50
74
74
98
98
122
122
146
146
170
170
1
2
3
4
12
20
28
36
44
52
60
4
12
20
28
36
44
52
60
4
12
20
28
36
44
52
60
4
12
20
28
36
44
52
60
5
13
21
29
37
45
53
61
5
13
21
29
37
45
53
61
5
13
21
29
37
45
53
61
5
13
21
29
37
45
53
61
5
13
21
29
37
45
53
61
5
13
21
29
37
45
53
61
6
14
22
30
38
46
54
62
6
14
22
30
38
46
54
62
6
14
22
30
38
46
54
62
6
14
22
30
38
46
54
62
6
14
22
30
38
46
54
62
6
14
22
30
38
46
54
62
7
15
23
31
39
47
55
63
7
15
23
31
39
47
55
63
7
15
23
31
39
47
55
63
7
15
23
31
39
47
55
63
7
15
23
31
39
47
55
63
7
15
23
31
39
47
55
63
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
8
31
55
79
8
31
55
79
8
31
55
79
8
31
55
79
9
32
56
80
9
32
56
80
9
32
56
80
9
32
56
80
10
33
57
81
10
33
57
81
10
33
57
81
10
33
57
81
12 13
34 35 36 37
58 59 60 61
82 83 84 85
CTL 11 12 13
34 35 36 37
58 59 60 61
82 83 84 85
CTL 11 12 13
34 35 36 37
58 59 60 61
82 83 84 85
CTL 11 12 13
34 35 36 37
58 59 60 61
82 83 84 85
14
38
62
86
14
38
62
86
14
38
62
86
14
38
62
86
15
39
63
87
15
39
63
87
15
39
63
87
15
39
63
87
16
40
64
88
16
40
64
88
16
40
64
88
16
40
64
88
17
41
65
89
17
41
65
89
17
41
65
89
17
41
65
89
18
42
66
90
18
42
66
90
18
42
66
90
18
42
66
90
19
43
67
91
19
43
67
91
19
43
67
91
19
43
67
91
20
44
68
92
20
44
68
92
20
44
68
92
20
44
68
92
21
45
69
93
21
45
69
93
21
45
69
93
21
45
69
93
22
46
70
94
22
46
70
94
22
46
70
94
22
46
70
94
23
47
71
95
23
47
71
95
23
47
71
95
23
47
71
95
7
8
9
10
6
6
29
29
53
53
77
77
6
6
29
29
53
53
77
77
7
7
30
30
54
54
78
78
7
7
30
30
54
54
78
78
8
8
31
31
55
55
79
79
8
8
31
31
55
55
79
79
9
9
32
32
56
56
80
80
9
9
32
32
56
56
80
80
10
10
33
33
57
57
81
81
10
10
33
33
57
57
81
81
5
6
7
8
9
10
4
4
27
27
51
51
75
75
99
99
123
123
147
147
171
171
5
5
28
28
52
52
76
76
100
100
124
124
148
148
172
172
6
6
29
29
53
53
77
77
101
101
125
125
149
149
173
173
7
7
30
30
54
54
78
78
102
102
126
126
150
150
174
174
8
8
31
31
55
55
79
79
103
103
127
127
151
151
175
175
9
9
32
32
56
56
80
80
104
104
128
128
152
152
176
176
10
10
33
33
57
57
81
81
105
105
129
129
153
153
177
177
34
34
58
58
82
82
106
106
130
130
154
154
178
178
4
5
6
7
8
9
10
11
CTL CTL CTL
11
19
27
35
43
51
59
11
19
27
35
43
51
59
11
19
27
35
43
51
59
CTL CTL CTL
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
11
12
13
14
15
16
17
18
19
20
21
22
23
24
CTL 11
CTL 11
34
34
58
58
82
82
35
35
59
59
83
83
11
11
35
35
59
59
83
83
12
12
36
36
60
60
84
84
12
12
36
36
60
60
84
84
13
13
37
37
61
61
85
85
13
13
37
37
61
61
85
85
14
14
38
38
62
62
86
86
14
14
38
38
62
62
86
86
15
15
39
39
63
63
87
87
15
15
39
39
63
63
87
87
16
16
40
40
64
64
88
88
16
16
40
40
64
64
88
88
17
17
41
41
65
65
89
89
17
17
41
41
65
65
89
89
18
18
42
42
66
66
90
90
18
18
42
42
66
66
90
90
19
19
43
43
67
67
91
91
19
19
43
43
67
67
91
91
20
20
44
44
68
68
92
92
20
20
44
44
68
68
92
92
21
21
45
45
69
69
93
93
21
21
45
45
69
69
93
93
22
22
46
46
70
70
94
94
22
22
46
46
70
70
94
94
23
23
47
47
71
71
95
95
23
23
47
47
71
71
95
95
11
12
13
14
15
16
17
18
19
20
21
22
23
24
35
35
59
59
83
83
107
107
131
131
155
155
179
179
12
12
36
36
60
60
84
84
108
108
132
132
156
156
180
180
13
13
37
37
61
61
85
85
109
109
133
133
157
157
181
181
14
14
38
38
62
62
86
86
110
110
134
134
158
158
182
182
15
15
39
39
63
63
87
87
111
111
135
135
159
159
183
183
16
16
40
40
64
64
88
88
112
112
136
136
160
160
184
184
17
17
41
41
65
65
89
89
113
113
137
137
161
161
185
185
18
18
42
42
66
66
90
90
114
114
138
138
162
162
186
186
19
19
43
43
67
67
91
91
115
115
139
139
163
163
187
187
20
20
44
44
68
68
92
92
116
116
140
140
164
164
188
188
21
21
45
45
69
69
93
93
117
117
141
141
165
165
189
189
22
22
46
46
70
70
94
94
118
118
142
142
166
166
190
190
23
23
47
47
71
71
95
95
119
119
143
143
167
167
191
191
12
13
14
15
16
17
18
19
20
21
22
23
24
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
Port
34
34
58
58
82
82
CTL
CTL
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
1
2
3
4
5
6
7
8
Port
CTL 11
CTL 11
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Replicates
1
1
Port
CTL 11
Replicates
1
Format 384
(Cat. No.
Format
3844342265)
(P/N 4342265)
380 unique assays + 4 mandatory controls
380 unique assays + 4 mandatory controls
1 unique sample
1 unique sample
1
8
16
24
32
40
48
56
1
8
16
24
32
40
48
56
Replicates
2
Format 192
(Cat. No.
Format
1924346802)
(P/N 4346802)
191 unique
assays
+ 1assays
mandatory
control control
191
unique
+ 1 mandatory
1 unique sample
1 unique sample
1
8
16
24
32
40
48
56
1
8
16
24
32
40
48
56
Replicates
3
Format 96b
(Cat. No.
Format
96b4342261)
(P/N 4342261)
95 unique assays + 1 mandatory control
95 unique assays + 1 mandatory control
2 unique samples
2 unique samples
Port
1
8
16
24
32
40
48
56
1
8
16
24
32
40
48
56
1
2
3
4
5
6
7
8
Port
1 2 3 4 5 6 7 8 9 10 CTL CTL 11 12 13 14 15 16 17 18 19 20 21 22
23 24 25 26 27 28 29 30 31 32 CTL CTL 33 34 35 36 37 38 39 40 41 42 43 44
45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68
69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92
93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116
117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140
141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164
165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188
189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212
213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236
237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260
261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284
285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308
309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332
333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356
357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
1
2
3
4
5
6
7
8
24
127MI33-06
1. Load
2. Spin
3. Seal
4. Run
5 minutes to complete steps 1 through 3
Figure 3. The TaqMan Array Card streamlines the reaction setup process in four simple steps. Loading the array with your cDNA sample and TaqMan® Universal Master
Mix II, then spinning and sealing the card, typically takes only five minutes.
®
Predesigned gene signature cards
are also available
If you do not have the time to compose a
list of assays, use one of our predesigned
TaqMan® Array Gene Signature Cards.
TaqMan® Array Gene Signature Cards
contain select TaqMan® Gene Expression
Assays for focused gene sets for many
important target classes and pathways.
Genes in each of the arrays have been
culled from pathway analysis tools,
published review papers, and collaborator
and customer input. Select from a variety
of TaqMan® Array Gene Signature Cards
covering areas such as GPCRs, immune
response, or protein kinases at our website
at lifetechnologies.com/signaturecards. You
can also use our Endogenous Control Arrays
to assess which housekeeping genes are
best for your specific study.
Simple and straightforward loading
Once you receive your TaqMan® Array Card,
using it is fast and simple. The TaqMan®
Array Card has eight sample-loading ports,
each connecting to 48 reaction wells. Simply
combine 30–1000 ng of cDNA sample with
TaqMan® Universal Master Mix II (for each
port), pipette sample mix into the TaqMan®
Array Card, centrifuge briefly to fill the wells,
and seal the card. Within minutes, your
384-well TaqMan® Array Card is ready to run
on the QuantStudio™ 12K Flex, ViiA™ 7,
or 7900HT Real-Time PCR System
(Figure 3). Imagine how much time and
costs you can save by not having to set up
individual reactions, as well as reducing
the the risk of introducing variability.
Accurate, quantitative gene expression
results are available when thermal cycling is
complete. The reduced number of pipetting
steps streamlines the reaction setup
process and eliminates the need for
liquid-handling robotics.
Applied Biosystems® real-time
PCR instruments
TaqMan® Array Cards function seamlessly
with the Applied Biosystems® QuantStudio™
12K Flex, ViiA™ 7, and 7900HT Real-Time
PCR Systems. The new QuantStudio™
12K Flex Real-Time PCR System offers
unprecedented throughput, flexibility, and
scalability and is also capable of running
TaqMan® OpenArray® Plates. The ViiA™
7 Real-Time PCR System also features
interchangeable blocks as well as 21
filter combinations. The 7900HT Fast
Real-Time PCR System has been used
to run TaqMan® Array Cards to generate
extensively published and trusted gene
expression results.
Service and support
Highly trained Service Engineers are
available to install the TaqMan® Array Card
block on your QuantStudio™ 12K Flex, or ViiA™
7 Real-Time PCR System and get you up and
running quickly. In addition, our Technical
Support group can assist with any questions
you may have. For more information, contact
your Life Technologies sales representative
or distributor.
Powerful data analysis
Our DataAssist™ Software package enables
rapid and comprehensive interpretation of
Custom TaqMan® Array Card results.
DataAssist™ Software provides a filtering
procedure for outlier removal, various
normalization methods based on single or
multiple genes, and relative quantification
(RQ) analysis of gene expression through a
combination of statistical analysis and
interactive visualization. DataAssist™
Software can be downloaded for free
at lifetechnologies.com/dataassist.
Ordering information
Description
No. of assays
No. of samples per card
1 replicate
2 replicates
3 replicates
Minumum order
quantity
Cat. No.
10
4342247
10
4346798
10
4342249
10
4346799
10
4342253
10
4346800
4 replicates
Custom TaqMan® Array Card (384-well microfluidic card)
Format 12
11 + 1 control
Format 16
15 + 1 control
8
Format 24
23 + 1 control
Format 32
31 + 1 control
Format 48
47 + 1 control
Format 64
63 + 1 control
Format 96a
95 + 1 control
2
10
4342259
Format 96b
95 + 1 control
2
10
4342261
Format 192
191 + 1 control
1
10
4346802
Format 384
380 + 4 controls
10
4342265
8
8
4
4
4
2
2
1
TaqMan® Array Card Blocks*
QuantStudio™ 12K Flex TaqMan® Array Card Block
4453546
ViiA 7 TaqMan Array Card Block
4453546
7900HT TaqMan® Array Card Block
4329012
™
®
* Each TaqMan® Array Card Block includes sample block, TaqMan® Array Card sealer, custom buckets and adaptors, Getting Started guide, and chemical installation kit. A compatible
centrifuge and rotor are required, but are not supplied. Ask your local sales representative for a list of compatible centrifuge and rotor options.
For more information and full terms of the TaqMan® Assays QPCR Guarantee,
visit lifetechnologies.com/taqmanguarantee.
© 2012 Life Technologies Corporation. All rights resserved. The trademarks mentioned herin are the property of Life Technologies Corporation or their respective owners. TaqMan® is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. Printed in the USA. CO01513 0112