Facility and Resources-Stem Cell Core Facility (SCCF)
General Introduction The Stem Cell Core Facility (SCCF) at The Johns Hopkins
Institute for Cell Engineering (ICE) was opened September 2011 to help human
stem cell researchers. The missions of SCCF is to maintain stem cell lines
(hES/hiPS), to provide researchers with stem cell lines and related materials, to
perform validation and quality-control of stem cell lines, to provide the technology
for stem cell derivation, culture, differentiation and genetic modification, and to
train and assist end users with stem cell related techniques. The facility will provide
the community with services ranging from simple hESC maintenance to advanced
techniques on a fee per service basis.
Personnel Director: Valina L. Dawson, Ph.D.
Co-Director: Hong-jun Song, Ph.D.
Manager: Hyesoo Kim, Ph.D.
Research technologist: Shravya Kommineni, M.S.
Laboratory space The Laboratory is located on 7th floor of Edward Miller Research
Building. It consists of 1000 sq ft of space and contains a tissue culture room, bench
workspace and an office. In addition, a centrifugation and storage room, microscopy
room and a walk-in cold room are available as common space. Security access is in
place for the TC room.
Equipment Four class II biosafety cabinets including one for virus work, eight CO2
incubators, one IVF station with two attached stereoscopies and a monitoring
system, one centrifuge and two inverted microscopies are housed in the tissue
culture room. A PCR machine and electrophoresis system are available for RT-PCR
and two rockers for immunostaining are housed in the non-TC room. For storage of
biological samples, we have one -80°C deep freezer, one liquid nitrogen freezer
which maintains up to 20,000 frozen vials, two 4°C refrigerators and one -20°C
freezer. Flow cytometry and gel doc system apparatus are also available as a
common resource of ICE.
Services Providing different types of cells
Custom hiPSC generation
Differentiation of hES/hiPS cells
Genetic modification of hES/hiPS cells
Individual and group training courses
Other custom experiments
Others Human cells and viruses are registered annually with the JHU Biosafety Office
and are handled under authorization of the Johns Hopkins Institutional Stem Cell
Research Oversight (ISCRO).