3rd Korea-UA Nano-forum (2006)
Fabrication of Non-Biofouling
Polyethylene Glycol
Micro- and Nanochannels
Pilnam Kim, Hoon Eui Jeong, and Kahp Yang Suh
Assistant Professor
School of Mechanical and Aerospace Engineering
Seoul National University
http://nadeb.snu.ac.kr
[email protected]
Seoul National Univ.
School of Mech. & Aero. Eng.
Introduction and Background
Microarray
Microarray
or
or
LOC
LOC
Template
Templatefabrication
fabrication
Needs surface modification
for bioassay or biosensor
PEG!
How to prevent biofouling?
http://www.accessexcellence.org
http://www.accessexcellence.org
• Protein repellent property
O
Biomolecule-array
Biomolecule-array
O
O
n
O
Proteins,
Cells
PEG
coating
Template
Template
material
material
Fabrication
Fabrication
method
method
Cell,
Cell,protein,
protein,lipid
lipid
membrane,
DNA
membrane, DNA
Choice of Material !!
PDMS
Biofouling!
Silicon
Non-biocompatible
Glass
Non-specific binding
Cell
PEG
† PEG (polyethylene glycol)
• Good bio-compatibility
• Non-biofouling
(Resistance against protein and cell
adsorption)
• High resolution (sub 100 nm)
• Fast & easy prototyping
• Transparency
• Hydrophilic: Pumpless
Fabrication of PEG Channels
PEG + Micro/Nanofabrication
(UV-assisted molding)
= PEG micro/nano fluidic device
Nano
fabrication
using PEG
Results and discussion
▶ Channel fabrication
▶ Non-biofouling
µ-channel
Cell
100 µm
20 µm
nano-channel
▶ Pumpless : Hydrophilic channel
3µm
▶ PEG-Microfluidic device
10 µm
PEG-device
PDMS-device