Suppl. Table 3S
Effects of amino acids, thiol and chelator reagents
¹ Full activities (100%) of UGT72B11, UGT90A7, or UGT95A1 correspond to 3.6, 1.9, and 17.5
pkat µg-1, respectively
Relative activity (%)¹
Concentration
Control
[mM]
-
UGT72B11
UGT90A7
UGT95A1
100
100
100
DEPC (histidine modifying agent)
1
3
8
9
PMSF (serine modifying agent)
NEM (thiol alkylating agent)
DIDS (lysine modifying agent)
EDTA (chelating agent)
1
1
1
1
5
118
43
0
87
91
50
48
2
92
105
99
102
3
96
110
50
46
88
100
5
43
100
96
2-Mercaptoethanol (disulphide
bond reducer)
DTT (disulphide bond reducer)