Supporting Information
Sensitive analysis of 33 free amino acids in serum, milk and muscle by
ultra-high Performance liquid chromatography-quadrupole-orbitrap high
resolution mass spectrometry
Bingjie Yin, *† Tingting Li, *† Siran Zhang ‡, Zhen Li&, and Pingli He**,†
†
State Key Laboratory of Animal Nutrition, College of Animal Science and
Technology, China Agricultural University, Beijing 100193, PR China
‡
Beijing Huiwen Middle School, Beijing 100061, PR China
&
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological
Sciences, China Agricultural University, Beijing 100193, PR China
** Bingjie Yin and Tingting Li contributed equally to this work
*Corresponding author. State Key Laboratory of Animal Nutrition, College of Animal
Science and Technology, China Agricultural University, Beijing, 100193, China
E-mail address: [email protected].
Tel: + 86-10-62733588; fax: +86-10-62733688.
SFigure 1 Extracted ion chromatograms of AAs standard solution (50 mol/L for
each compound) obtained from BEH C18 column (A) and HILIC C18 column (B). a.
Serine; b. Histidine; c. Tyrosine; d. Isoleucine; e. Leucine.
SFigure 2 Typical extracted ion chromatograms of AAs standard solution (50
mol/L for each compound) derived by AQC (A) and PITC (B). a. Threonine; b.
Tyrosine; c. Methionine; d. Tryptophan.
SFigure 3. Optimization of derivatization condition. (A) borate buffer dosage, (B)
AQC dosage.
STable 1. Free Amino acid concentration in real samples.
P value
Muscle
(nmol/g)
P value
Alanine
Serum
(mol/L)
150.53±3.17
P value
0.36
Milk
(mol/L)
11.56±2.76
0.19
2098.59±185.27
Arginine
101.71±46.41
0.13
159.30±34.47
0.18
6.17±1.53
0.34
Aspartic Acid
48.55±5.47
0.22
154.58±33.22
0.56
8.28±3.04
0.01
Cystine
64.49±34.35
0.08
ND
0.37±0.17
0.11
Glutamic Acid
134.32±38.75
0.15
815.92±191.60
0.15
71.94±15.92
0.12
Glycine
461.54±112.01
0.77
1906.52±227.03
0.10
37.07±13.30
0.38
Histidine
34.55±8.44
0.04
13.29±3.76
0.02
3.04±0.84
0.16
Isoleucine
90.15±15.57
0.25
85.33±14.41
0.08
4.20±0.82
0.51
Leucine
161.33±15.40
0.06
170.16±19.18
0.16
14.37±2.14
0.44
Lysine
236.86±38.69
0.42
381.91±56.16
0.32
6.08±1.55
0.07
Methionine
279.9±19.97
0.89
54.95±12.54
0.01
1.40±0.38
0.50
Phenylalanine
71.22±5.33
0.68
73.93±15.99
0.09
4.04±0.72
0.08
Proline
175.90±31.75
0.93
183.09±23.89
0.76
28.26±5.26
0.11
Serine
92.01±19.00
0.35
156.79±42.87
0.33
12.47±1.58
0.26
Threonine
181.52±32.08
0.01
151.39±40.18
0.02
7.07±1.36
0.24
Tryptophan
126.80±25.40
0.02
22.73±3.72
0.10
1.63±0.53
0.06
Tyrosine
231.2±30.10
0.10
50.10±8.42
0.26
4.20±1.11
0.11
Valine
200.36±29.22
0.27
183.66±19.71
0.32
11.31±1.93
0.19
Phosphoserine
1.07±2.61
0.08
ND
Taurine
79.81±16.55
0.5
3325.40±454.58
O-Phosphorylethanolamine 16.56±3.71
0.36
ND
Compound
0.17
ND
0.72
314.91±27.91
0.10
ND
Hydroxyproline
55.39±8.24
0.52
72.95±13.16
0.48
1.24±0.67
0.20
Asparagine
175.74±35.92
0.18
137.56±9.03
0.34
2.21±0.49
0.34
Glutamine
385.29±53.38
0.45
1289.9±181.58
0.27
17.29±9.04
0.01
Aminoadipate
3.08±1.46
0.12
84.29±26.70
0.11
7.60±2.24
0.56
Citrulline
50.78±6.17
0.61
80.63±18.32
0.07
1.29±0.60
0.38
Aminobutyric acid
9.06±2.14
0.31
19.72±4.87
0.17
0.25±0.08
0.85
β-Aminobutyric acid
9.01±2.17
0.15
19.48±2.65
0.42
0.26±0.09
0.09
γ-Aminobutyric acid
498.90±119.0
0.08
20.74±1.02
0.13
0.30±0.08
0.12
1-Methylhistidine
3.87±1.10
0.01
0.36±0.03
0.04
0.09±0.04
0.04
3-Methylhistidine
5.86±2.00
0.02
0.49±0.13
0.08
0.18±0.07
0.06
Carnosine
80.36±57.80
0.77
8956.28±832.12
0.66
0.43±0.09
0.10
Ornithine
122.70±11.13
0.48
130.23±21.47
0.52
2.53±0.97
0.12
ND, none detected.