Environmental Science and Pollution Research
Spatio-temporal dynamics of bacterial and archaeal communities in
household biogas digesters from tropical and subtropical regions of
Yunnan province, China
Guangliang Tian1,2,3†, Qiumin Li1,2,3†, Minghua Dong4, Yan Wu4, Bin Yang1,2,3, Lijuan
Zhang1,2,3, Yingjuan Li1,2,3, Fang Yin1,2,3, Xingling Zhao1,2,3, Yongxia Wang4, Wei
Xiao4, Xiaolong Cui4*, Wudi Zhang1,2,3*
1
Yunnan Research Center of Biogas Technology and Engineering, Yunnan Normal
University, Kunming 650500, PRC
2
Engineering and Research Center of Sustainable Development and Utilization of
Bioenergy, Ministry of Education, Yunnan Normal University, Kunming 650500, PRC
3
Yunnan Key Laboratory of Rural Energy Engineering, Yunnan Normal University,
Kunming 650500, PRC
4
Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PRC
†
These authors equally contributed to this work.
*Corresponding authors:
Wudi Zhang: email: [email protected]
Xiaolong Cui: email: [email protected]
Table S1 Taxonomic relationships between sample B5S bacterial 16S rRNA gene sequences and those in the NCBI database
OUT
Sequence
Sequence
Accession
Similar
Taxonomic
ity
group
Closest relative
number
amount
Proportion (%)
number
1
18
5.45
CU922607.1
Uncultured Chloroflexi bacterium
99%
Chloroflexi
2
4
1.21
CU925778.1
Uncultured Chloroflexi bacterium
99%
Chloroflexi
3
3
0.91
HQ689260.1
Anaerolineaceae bacterium enrichment
97%
Chloroflexi
Chloroflexi
culture clone B6_153
4
1
0.30
HM992543.1
uncultured Chloroflexi bacterium
98%
5
4
1.21
NR_040971.1
Leptolinea tardivitalis
97%
6
1
0.30
EU522649.1
uncultured Chloroflexi bacterium
97%
7
3
0.91
CU926844.1
Uncultured Chloroflexi bacterium
99%
Chloroflexi
8
1
0.30
HQ183894.1
uncultured Bellilinea sp.
99%
Chloroflexi
9
2
0.61
JN868246.1
uncultured Chloroflexi bacterium
95%
Chloroflexi
10
1
0.30
JN868246.1
uncultured Chloroflexi bacterium
94%
Chloroflexi
11
2
0.61
CU924589.1
Uncultured Chloroflexi bacterium
99%
Chloroflexi
12
1
0.30
JQ292916.1
Ornatilinea apprima
94%
Chloroflexi
13
2
0.61
AB293397.1
uncultured Chloroflexi bacterium
98%
Chloroflexi
14
1
0.30
GU236040.1
uncultured Chloroflexi bacterium
95%
Chloroflexi
15
2
0.61
FJ516928.1
uncultured Chloroflexi bacterium
96%
Chloroflexi
16
9
2.73
JN998190.1
17
3
0.91
HQ183889.1
18
2
0.61
AB780890.1
uncultured Chloroflexi bacterium
97%
Chloroflexi
19
1
0.30
HM992547.1
uncultured Chloroflexi bacterium
96%
Chloroflexi
20
1
0.30
AJ431246.1
Dehalococcoides sp. BHI80-15
87%
Chloroflexi
21
5
1.52
KC836757.1
uncultured Chloroflexi bacterium
97%
Chloroflexi
22
1
0.30
DQ432275.1
uncultured Chloroflexi bacterium
94%
Chloroflexi
23
1
0.30
FJ949218.1
uncultured Chloroflexi bacterium
96%
Chloroflexi
24
1
0.30
FJ949428.1
uncultured Chloroflexi bacterium
91%
Chloroflexi
25
1
0.30
AB433076.1
uncultured Chloroflexi bacterium
91%
26
5
1.52
EU887989.1
uncultured Armatimonadetes bacterium
100%
Armatimonadetes
27
1
0.30
JN540113.1
uncultured Armatimonadetes bacterium
95%
Armatimonadetes
28
1
0.30
AB742096.1
uncultured Armatimonadetes bacterium
89%
Armatimonadetes
29
56
16.97
GQ133228.1
uncultured bacterium
99%
bacteria
30
4
1.21
CU918284.1
uncultured bacterium
99%
bacteria
31
2
0.61
NR_102776.1
Syntrophus aciditrophicus SB
99%
Proteobacteria
32
1
0.30
JQ346736.1
Syntrophus buswellii
99%
Proteobacteria
33
8
2.42
HQ133035.1
98%
Proteobacteria
uncultured Chloroflexi bacterium
uncultured Chloroflexi bacterium
90%
90%
Chloroflexi
Chloroflexi
Chloroflexi
Chloroflexi
Chloroflexi
Smithella sp. enrichment culture clone
B312125
34
1
0.30
AJ133795.1
Syntrophus sp.
95%
Proteobacteria
35
1
0.30
AJ133795.1
Syntrophus sp.
96%
Proteobacteria
36
4
1.21
AB780938.1
99%
Proteobacteria
37
1
0.30
CU922073.1
100%
Deltaproteobacteria
99%
Proteobacteria
uncultured proteobacterium
Uncultured
Deltaproteobacteria
bacterium
38
1
0.30
FJ517130.1
uncultured
Desulfuromonadales
OUT
Sequence
Sequence
Accession
Similar
Taxonomic
ity
group
Proteobacteria
Closest relative
number
amount
Proportion (%)
number
bacterium
39
1
0.30
FJ485400.1
uncultured delta proteobacterium
97%
40
1
0.30
AB265860.2
uncultured Myxococcales bacterium
96%
41
1
0.30
FR749898.1
Desulfovibrio giganteus
91%
42
3
0.91
JQ906964.1
uncultured Candidatus Cloacamonas sp.
99%
bacteria
43
7
2.12
GQ133094.1
uncultured bacterium
100%
bacteria
44
1
0.30
FJ484513.1
uncultured planctomycete
92%
Planctomycetes
45
1
0.30
FR836444.1
uncultured Verrucomicrobia bacterium
96%
Verrucomicrobia
46
1
0.30
AB329652.1
uncultured bacterium
99%
bacteria
47
1
0.30
AB780894.1
99%
Actinobacteria
48
1
0.30
HQ162762.1
uncultured actinomycete
97%
Actinobacteria
49
1
0.30
KC605005.1
uncultured bacterium
96%
bacteria
50
2
0.61
JN530357.1
uncultured organism
92%
uncultured organism
51
2
0.61
CU922104.1
uncultured bacterium
100%
bacteria
97%
Deltaproteobacteria
99%
Proteobacteria
97%
Deltaproteobacteria
uncultured actinobacterium
Uncultured
52
1
0.30
Proteobacteria
Proteobacteria
Deltaproteobacteria
CU925786.1
bacterium
uncultured
53
1
0.30
Syntrophorhabdaceae
AJ009498.1
bacterium SJA-162
Uncultured
54
3
0.91
Deltaproteobacteria
CU922985.1
bacterium
uncultured
55
3
0.91
Syntrophorhabdaceae
AJ249102.1
99%
Proteobacteria
bacterium SHA-22
56
3
0.91
HQ183795.1
uncultured Pelospora sp.
99%
Firmicutes
57
1
0.30
NR_044008.1
Syntrophomonas zehnderi OL-4
96%
Firmicutes
58
1
0.30
JN039016.1
uncultured Acidobacteria bacterium
98%
59
1
0.30
GQ859841.1
uncultured bacterium
97%
bacteria
60
1
0.30
EU245287.1
uncultured organism
85%
uncultured organism
61
5
1.52
EU887787.1
uncultured Clostridium sp.
98%
Firmicutes
62
1
0.30
AY548783.1
Clostridium sp. PPf35E6
94%
Firmicutes
63
4
1.21
DQ811926.1
uncultured Firmicutes bacterium
100%
Firmicutes
64
1
0.30
NR_074536.1
Clostridium lentocellum DSM 5427
97%
Firmicutes
65
1
0.30
HM566769.1
98%
Firmicutes
66
2
0.61
GU370096.1
Clostridium sp. P530(1)
95%
Firmicutes
67
3
0.91
DQ298123.1
Clostridium sp. U523B3
98%
Firmicutes
68
1
0.30
DQ855943.1
Clostridium disporicum
99%
Firmicutes
69
2
0.61
HQ003636.1
uncultured Firmicutes bacterium
97%
Firmicutes
70
4
1.21
JX576061.1
uncultured Clostridiales bacterium
97%
Firmicutes
71
1
0.30
GU112206.1
uncultured Firmicutes bacterium
99%
Firmicutes
72
3
0.91
EU551106.1
uncultured Clostridia bacterium
99%
Firmicutes
73
2
0.61
JQ087115.1
96%
Firmicutes
74
1
0.30
AB093546.1
92%
Firmicutes
75
2
0.61
FJ517038.1
95%
Firmicutes
Bacillus sp. SGE69(2010)
uncultured Acetivibrio sp.
Clostridium sp. JC3
uncultured Firmicutes bacterium
Acidobacteria
OUT
Sequence
Sequence
Accession
Similar
Taxonomic
ity
group
Closest relative
number
amount
Proportion (%)
number
76
5
1.52
JX505390.1
uncultured Clostridiales bacterium
97%
Firmicutes
77
1
0.30
AB742054.1
uncultured Firmicutes bacterium
97%
Firmicutes
78
2
0.61
AB234462.1
uncultured Clostridiales bacterium
93%
Firmicutes
79
1
0.30
NR_042834.1
Alkalibacter saccharofermentans
90%
Firmicutes
80
4
1.21
JX575962.1
uncultured Synergistetes bacterium
99%
Synergistetes
81
1
0.30
AB603841.1
97%
Synergistetes
82
1
0.30
AB780941.1
99%
Synergistetes
83
1
0.30
KC800693.1
Mesotoga sp. VNs100
99%
Thermotogae
84
1
0.30
NR_029020.1
Thermosipho atlanticus
85%
Thermotogae
85
1
0.30
FJ824045.1
uncultured planctomycete
94%
Planctomycetes
86
1
0.30
HQ133208.1
98%
Planctomycetes
Planctomycetes
uncultured Synergistetes bacterium
uncultured Synergistetes bacterium
Planctomycete enrichment culture clone
B419
87
1
0.30
AB583310.1
uncultured planctomycete
91%
88
6
1.82
FM176310.1
uncultured Petrimonas sp.
85%
Bacteroidetes
89
20
6.06
KC502889.1
uncultured Bacteroides sp.
100%
Bacteroidetes
90
1
0.30
HM992551.1
uncultured Bacteroidetes bacterium
97%
Bacteroidetes
91
15
4.55
GU472715.1
uncultured Bacteroidales bacterium
94%
Bacteroidetes
92
4
1.21
JQ012284.1
uncultured Bacteroidetes bacterium
100%
Bacteroidetes
93
14
4.24
HQ183940.1
uncultured Bacteroidetes bacterium
98%
Bacteroidetes
94
3
0.91
FJ516908.1
uncultured Cytophagales bacterium
99%
Bacteroidetes
95
4
1.21
FJ213814.1
uncultured Bacteroidetes bacterium
97%
Bacteroidetes
96
1
0.30
HQ003601.1
uncultured Bacteroidetes bacterium
96%
Bacteroidetes
97
2
0.61
HQ183934.1
uncultured Bacteroidetes bacterium
98%
Bacteroidetes
98
1
0.30
JX576072.1
uncultured Bacteroidetes bacterium
99%
Bacteroidetes
99
1
0.30
GU056890.1
91%
Bacteroidetes
100
1
0.30
JN998178.1
97%
Bacteroidetes
101
2
0.61
AB742056.1
uncultured Bacteroidetes bacterium
99%
Bacteroidetes
102
1
0.30
EU794138.1
uncultured Bacteroidetes bacterium
95%
Bacteroidetes
103
1
0.30
JN873215.1
uncultured Parabacteroides sp.
99%
Bacteroidetes
104
1
0.30
GQ348350.1
uncultured Bacteroidetes bacterium
88%
Bacteroidetes
105
1
0.30
JN002829.1
99%
Fusobacteria
106
1
0.30
EU308574.1
Deinococcus sp. 4A6
97%
Deinococcus-Thermus
107
1
0.30
CU926913.1
Uncultured Chlorobi bacterium
99%
Chlorobi
108
1
0.30
FJ269064.1
97%
bacteria
uncultured Bacteroidetes bacterium
uncultured Bacteroidetes bacterium
uncultured Fusobacterium sp.
iron-reducing
bacterium
culture clone HN67
enrichment
Table S2 Taxonomic relationships between sample B5S archaeal 16S rRNA gene sequences and those in the NCBI database
Sequence
OUT
Sequence
Accessible
Proportion
number
amount
Closest relative
Taxonomic group
uncultured Methanosarcinaceae
Methanosarcinacea
Similarity
resource
number
(%)
1
2
1.08
JQ079960.1
archaeon
Methanospirillum
2
2
1.08
the anoxic sediment of the
98%
e
'killer lake' Lake Monoun
hungatei
NR_074177.1
Methanospirillum
98%
Methanospirillum sp. X-18
Methanospirillum
97%
uncultured Methanomicrobiales
Methanomicrobiale
archaeon
s
-
JF-1
3
4
1
1
0.54
0.54
KF153052.1
KF198699.1
Madoi soil
cellulose-degrading
94%
enrichment cultures
5
132
71.35
NR_102903.1
Methanosaeta concilii GP6
Methanosaeta
99%
6
6
3.24
KF431940.1
Methanosaeta concilii
Methanosaeta
98%
7
2
1.08
NR_041665.1
Methanolobus
97%
soil horizon of paddy fields
deep subsurface sediments in
Methanolobus profundi
a natural gas field
8
2
1.08
NR_074221.1
Methanosarcina mazei Go1
Methanosarcina
100%
-
9
9
4.86
HQ141835.1
uncultured euryarchaeote
euryarchaeote
99%
leachate sediment
10
11
5.95
KC661944.1
uncultured crenarchaeote
crenarchaeote
99%
root surfaces
11
2
1.08
JX576137.1
uncultured crenarchaeote
crenarchaeote
99%
pit mud
12
1
0.54
KC661852.1
uncultured crenarchaeote
crenarchaeote
99%
13
3
1.62
FJ968086.1
uncultured crenarchaeote
crenarchaeote
99%
14
2
1.08
JN052741.1
uncultured crenarchaeote
crenarchaeote
99%
root surfaces
cold sulfur-rich spring
anaerobic
digestion
of
animal waste
root surfaces of Ruppia spp.
15
6
3.24
KC661655.1
uncultured crenarchaeote
crenarchaeote
98%
and Phragmites australis
16
2
1.08
HQ141842.1
uncultured crenarchaeote
crenarchaeote
98%
17
1
0.54
KC661657.1
uncultured crenarchaeote
crenarchaeote
97%
leachate sediment
root surfaces of Ruppia spp.
and Phragmites australis
Table S3 Taxonomic relationships between sample J8S bacterial 16S r RNA gene sequences and those in the NCBI database
OUT
Sequence
Sequence
Accession
number
amount
proportion(%)
number
1
2
0.57
EU522649.1
2
1
0.29
3
1
4
Closest relative
Similarity
Taxonomic group
uncultured Chloroflexi bacterium
97%
Chloroflexi
JN596366.1
uncultured Chloroflexi bacterium
99%
Chloroflexi
0.29
HM992544.1
uncultured Chloroflexi bacterium
99%
Chloroflexi
1
0.29
GU236052.1
uncultured Chloroflexi bacterium
98%
Chloroflexi
5
1
0.29
JX023230.1
uncultured Chloroflexi bacterium
98%
Chloroflexi
6
1
0.29
NR_040971.1
Leptolinea tardivitalis
97%
Chloroflexi
7
23
6.57
EU887788.1
uncultured Bellilinea sp.
98%
Chloroflexi
8
12
3.43
JF754541.1
99%
Chloroflexi
97%
Chloroflexi
uncultured Chloroflexi bacterium
Anaerolineaceae
9
2
0.57
bacterium
enrichment
HQ689260.1
culture clone B6_153
10
1
0.29
HM992543.1
uncultured Chloroflexi bacterium
98%
Chloroflexi
11
5
1.43
EU522645.1
uncultured Chloroflexi bacterium
98%
Chloroflexi
12
1
0.29
JN217032.1
uncultured Bellilinea sp.
98%
Chloroflexi
13
1
0.29
CU922908.1
Uncultured Chloroflexi bacterium
99%
14
1
0.29
AB293397.1
uncultured Chloroflexi bacterium
93%
Chloroflexi
15
1
0.29
GU236040.1
uncultured Chloroflexi bacterium
97%
Chloroflexi
16
1
0.29
GU236040.1
uncultured Chloroflexi bacterium
97%
Chloroflexi
17
1
0.29
HM992546.1
uncultured Chloroflexi bacterium
98%
Chloroflexi
18
1
0.29
JN409044.1
uncultured Chloroflexi bacterium
98%
Chloroflexi
19
4
1.14
JN998190.1
uncultured Chloroflexi bacterium
89%
Chloroflexi
20
10
2.86
JN998190.1
uncultured Chloroflexi bacterium
87%
Chloroflexi
21
4
1.14
FJ516928.1
uncultured Chloroflexi bacterium
96%
Chloroflexi
22
3
0.86
AB780890.1
uncultured Chloroflexi bacterium
99%
Chloroflexi
23
1
0.29
AB780890.1
uncultured Chloroflexi bacterium
96%
Chloroflexi
24
1
0.29
HM992547.1
uncultured Chloroflexi bacterium
99%
Chloroflexi
25
9
2.57
KC836757.1
uncultured Chloroflexi bacterium
97%
Chloroflexi
26
1
0.29
HQ183887.1
uncultured Chloroflexi bacterium
99%
Chloroflexi
27
1
0.29
EF613805.1
uncultured Chloroflexi bacterium
94%
Chloroflexi
28
2
0.57
AY559074.1
PCB-dechlorinating bacterium OTU-11
97%
bacteria
29
1
0.29
AY559069.1
PCB-dechlorinating bacterium OTU-6
99%
bacteria
30
2
0.57
AB780944.1
uncultured Synergistetes bacterium
99%
Synergistetes
31
1
0.29
AB623229.1
Aminivibrio pyruvatiphilus
99%
Synergistetes
32
1
0.29
JX301643.1
uncultured Synergistetes bacterium
99%
Synergistetes
33
1
0.29
EU721789.1
uncultured Synergistes sp.
99%
Synergistetes
34
2
0.57
KC800693.1
Mesotoga sp. VNs100
99%
Thermotogae
35
1
0.29
NR_029020.1
Thermosipho atlanticus
85%
Thermotogae
36
1
0.29
EU708506.1
uncultured bacterium
97%
37
2
0.57
EU887989.1
uncultured Armatimonadetes bacterium
100%
Chloroflexi
bacteria
Armatimonadetes
OUT
Sequence
Sequence
Accession
number
amount
proportion(%)
number
38
1
0.29
39
1
40
Closest relative
Similarity
Taxonomic group
GU236092.1
uncultured Armatimonadetes bacterium
97%
Armatimonadetes
0.29
JN540113.1
uncultured Armatimonadetes bacterium
96%
Armatimonadetes
1
0.29
AY607137.1
uncultured Armatimonadetes bacterium
96%
Armatimonadetes
41
20
5.71
KC502889.1
99%
Bacteroidetes
42
1
0.29
HM992551.1
97%
Bacteroidetes
43
1
0.29
HQ183940.1
96%
Bacteroidetes
44
3
0.86
JQ012284.1
uncultured Bacteroidetes bacterium
99%
Bacteroidetes
45
14
4.00
HQ183940.1
uncultured Bacteroidetes bacterium
98%
Bacteroidetes
46
3
0.86
FJ213814.1
uncultured Bacteroidetes bacterium
97%
Bacteroidetes
47
1
0.29
FJ213814.1
uncultured Bacteroidetes bacterium
97%
Bacteroidetes
48
11
3.14
GU472715.1
uncultured Bacteroidales bacterium
94%
Bacteroidetes
49
1
0.29
JX576072.1
99%
Bacteroidetes
50
2
0.57
JQ580449.1
uncultured Bacteroidetes bacterium
99%
Bacteroidetes
51
1
0.29
EU551113.1
uncultured Bacteroidetes bacterium
99%
Bacteroidetes
52
1
0.29
GQ468575.1
uncultured Rikenellaceae bacterium
99%
Bacteroidetes
53
5
1.43
AB364869.1
uncultured bacterium
97%
bacteria
54
1
0.29
AJ306807.1
uncultured bacterium
97%
bacteria
55
1
0.29
EF451973.1
uncultured bacterium
93%
bacteria
56
1
0.29
AJ853530.1
uncultured bacterium
99%
bacteria
57
2
0.57
HQ860492.1
94%
bacteria
58
1
0.29
GQ261311.1
uncultured bacterium
96%
bacteria
59
15
4.29
GQ133094.1
uncultured bacterium
100%
bacteria
60
2
0.57
JQ906964.1
99%
bacteria
61
1
0.29
GQ182546.1
uncultured bacterium
98%
bacteria
62
1
0.29
JN039008.1
uncultured bacterium
98%
bacteria
63
3
0.86
DQ831550.1
uncultured delta proteobacterium
93%
Proteobacteria
64
1
0.29
FJ484835.1
uncultured delta proteobacterium
99%
Proteobacteria
65
11
3.14
GU112190.1
uncultured Syntrophus sp.
99%
Proteobacteria
66
2
0.57
AB669234.1
uncultured delta proteobacterium
99%
Proteobacteria
67
3
0.86
JQ996666.1
uncultured delta proteobacterium
99%
Proteobacteria
68
1
0.29
JX843908.2
uncultured delta proteobacterium
98%
Proteobacteria
69
1
0.29
AB723840.1
uncultured Syntrophus sp.
98%
Proteobacteria
70
1
0.29
JQ580155.1
uncultured delta proteobacterium
94%
Proteobacteria
71
1
0.29
CU922292.1
Uncultured Deltaproteobacteria bacterium
98%
bacteria
72
1
0.29
FJ484702.1
uncultured Spirochaetes bacterium
91%
Spirochaetes
73
3
0.86
JX271997.1
uncultured bacterium
95%
bacteria
74
1
0.29
JN998197.1
uncultured bacterium
99%
bacteria
75
2
0.57
JN530357.1
uncultured organism
91%
bacteria
76
1
0.29
GQ138699.1
uncultured organism
95%
bacteria
uncultured Bacteroides sp.
uncultured Bacteroidetes bacterium
uncultured Bacteroidetes bacterium
uncultured Bacteroidetes bacterium
uncultured bacterium
uncultured Candidatus Cloacimonas sp.
OUT
Sequence
Sequence
Accession
number
amount
proportion(%)
number
77
1
0.29
AB659093.1
78
1
0.29
DQ811803.1
uncultured delta proteobacterium
94%
Proteobacteria
79
4
1.14
JN688049.1
Clostridium sp. E2
98%
Firmicutes
80
2
0.57
GQ468578.1
uncultured Clostridium sp.
99%
Firmicutes
81
2
0.57
EU887787.1
uncultured Clostridium sp.
99%
Firmicutes
82
1
0.29
EU887984.1
uncultured Clostridia bacterium
90%
Firmicutes
83
1
0.29
EU029532.1
uncultured Firmicutes bacterium
95%
Firmicutes
84
1
0.29
JF319157.1
uncultured alpha proteobacterium
96%
Proteobacteria
85
1
0.29
JN540220.1
uncultured Clostridiales bacterium
97%
Firmicutes
86
1
0.29
FJ517038.1
uncultured Firmicutes bacterium
95%
Firmicutes
87
1
0.29
CU925649.1
Uncultured Firmicutes bacterium
99%
Firmicutes
88
2
0.57
JX505390.1
uncultured Clostridiales bacterium
97%
Firmicutes
89
2
0.57
AB742102.1
uncultured Firmicutes bacterium
97%
Firmicutes
90
2
0.57
DQ088231.1
uncultured bacterium
99%
bacteria
91
2
0.57
JQ668588.1
uncultured Firmicutes bacterium
98%
Firmicutes
92
1
0.29
GU112206.1
uncultured Firmicutes bacterium
99%
Firmicutes
93
1
0.29
KC555229.1
uncultured Clostridiales bacterium
97%
Firmicutes
94
1
0.29
JX576061.1
uncultured Clostridiales bacterium
97%
Firmicutes
95
6
1.71
HQ132405.1
uncultured Firmicutes bacterium
99%
Firmicutes
96
2
0.57
GQ868392.1
uncultured Clostridium sp.
100%
Firmicutes
97
1
0.29
AM159258.1
uncultured Clostridia bacterium
98%
Firmicutes
98
1
0.29
JN688045.1
Proteiniclasticum sp. N2
99%
Firmicutes
99
1
0.29
JX240762.1
uncultured Firmicutes bacterium
92%
Firmicutes
100
4
1.14
EU551106.1
uncultured Clostridia bacterium
98%
Firmicutes
101
1
0.29
EF562562.1
uncultured Clostridiales bacterium
95%
Firmicutes
102
1
0.29
JQ087115.1
uncultured Acetivibrio sp.
96%
Firmicutes
103
1
0.29
JF305754.1
uncultured Firmicutes bacterium
99%
Firmicutes
104
1
0.29
CU923060.1
Uncultured Firmicutes bacterium
97%
Firmicutes
105
1
0.29
JX575855.1
uncultured Firmicutes bacterium
96%
Firmicutes
106
6
1.71
JX575841.1
uncultured Pelospora sp.
97%
Firmicutes
107
2
0.57
HQ162762.1
98%
Actinobacteria
108
1
0.29
JN530962.1
uncultured organism
93%
unclassified sequences
109
1
0.29
GQ134229.1
uncultured bacterium
92%
bacteria
110
38
10.86
EU358731.1
uncultured bacterium
100%
bacteria
111
2
0.57
HQ184013.1
uncultured bacterium
97%
bacteria
112
2
0.57
AY261808.1
uncultured delta proteobacterium
98%
Proteobacteria
113
1
0.29
JX301602.1
uncultured Syntrophorhabdus sp.
94%
Proteobacteria
114
3
0.86
HE648210.1
uncultured Syntrophorhabdus sp.
97%
Proteobacteria
115
4
1.14
AJ249102.1
99%
Proteobacteria
Closest relative
uncultured organism
uncultured actinomycete
Similarity
99%
Taxonomic group
bacteria
uncultured Syntrophorhabdaceae bacterium
SHA-22
OUT
Sequence
Sequence
Accession
number
amount
proportion(%)
number
116
1
0.29
GQ183225.1
uncultured Desulfofaba sp.
97%
117
1
0.29
AB329652.1
uncultured bacterium
99%
bacteria
118
1
0.29
AJ390466.1
uncultured soil bacterium PBS-40
97%
Planctomycetes
119
1
0.29
AJ390466.1
uncultured soil bacterium PBS-41
97%
Planctomycetes
120
1
0.29
EU297349.1
uncultured planctomycete
99%
Planctomycetes
121
2
0.57
AJ390475.1
uncultured soil bacterium PRR-1B
92%
Planctomycetes
122
1
0.29
FJ788646.1
uncultured Planctomycetaceae bacterium
96%
Planctomycetes
123
2
0.57
GU120609.1
uncultured Planctomycetales bacterium
98%
Planctomycetes
124
1
0.29
HQ183982.1
uncultured planctomycete
99%
Planctomycetes
125
1
0.29
AB645223.1
uncultured planctomycete
93%
Planctomycetes
126
1
0.29
JQ087166.1
uncultured Planctomycetaceae bacterium
96%
Planctomycetes
127
1
0.29
GQ267140.1
94%
Planctomycetes
Closest relative
uncultured planctomycete
Similarity
Taxonomic group
Proteobacteria
Table S4 Taxonomic relationships between sample J8S archaeal 16S r RNA gene sequences and those in the NCBI database
OUT
Sequence
Sequence
Accessible
Closest relative
Similar
Taxonomic group
resource
number
amount
Proportion(%)
number
1
9
5.00
HQ141835.1
uncultured euryarchaeote
99%
Methanosaeta
8
112
62.22
NR_102903.1
Methanosaeta concilii GP6
99%
Methanosaeta
3
8
4.44
KF431940.1
Methanosaeta concilii
98%
Methanolobus
4
1
0.56
NR_041665.1
Methanolobus profundi
97%
Methanospirillum
5
5
2.78
NR_074177.1
Methanospirillum
98%
Methanospirillum
6
3
1.67
KF153052.1
98%
Methanospirillum
the anoxic sediment of lake
7
4
2.22
JQ079960.1
98%
Methanosarcinacea
the surface microlayer of corals
ity
hungatei
JF-1
Methanospirillum sp. X-18
uncultured
Methanosarcinaceae
e
archaeon
8
1
0.56
AY380641.1
uncultured
marine
91%
crenarchaeote
crenarchaeote
anaerobic digestion of animal
waste
9
5
2.78
JN052741.1
uncultured crenarchaeote
99%
crenarchaeote
cold sulfur-rich spring
10
6
3.33
FJ968085.1
uncultured crenarchaeote
98%
crenarchaeote
the anoxic sediment of the lake
11
1
0.56
JQ079952.1
uncultured crenarchaeote
97%
crenarchaeote
low-temperature sludges
12
1
0.56
EU662686.1
uncultured archaeon
98%
archaeon
root surfaces of Ruppia spp. and
Phragmites australis
13
12
6.67
KC661944.1
uncultured crenarchaeote
99%
crenarchaeote
root surfaces of Ruppia spp. and
Phragmites australis
14
3
1.67
KC661543.1
uncultured crenarchaeote
99%
crenarchaeote
pit mud
15
5
2.78
JX576093.1
uncultured crenarchaeote
100%
crenarchaeote
hot springs
16
2
1.11
HF922642.1
99%
archaeon
root surfaces of Ruppia spp. and
uncultured archaeon
Phragmites australis
17
1
0.56
18
1
0.56
KC661657.1
HQ141842.1
uncultured crenarchaeote
97%
crenarchaeote
leachate sediment
uncultured
98%
crenarchaeote
98%
crenarchaeote
leachate sediment
Fig. S1 The DGGE fingerprints of microbial communities from 19 digesters and in
different seasons. a and d represent the of and archaeal DGGE profiles, respectively,
from BN digesters; b and e represent those from JS; c and f represent those in summer
and winter (e.g., the letter S in marker B5S means samples collected in summer)
Fig. S2 The microbial communities cluster tree based on the DGGE fingerprints. a, b,
c, d, e, and f as in Fig. 1