Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 ISSN: 2319-7706 Volume 3 Number 3 (2014) pp. 291-302 http://www.ijcmas.com Original Research Article Influence of stocking density on growth performance of family chicks reared up to 18 weeks of age in under an intensive system Kenaleone Gabanakgosi*, John Cassius Moreki, Shalaulani James Nsoso and Christopher Mareledi Tsopito Department of Animal Science and Production, Botswana College of Agriculture, Private Bag 0027, Gaborone, Botswana *Corresponding author ABSTRACT Keywords Carcass characteristics; family chickens; growth parameters; rearing system; stocking density. The objective of the study was to evaluate the influence of stocking density on growth performance of family chicks reared up to 18 weeks of age under intensive system. A total of 248 day old chicks were randomly assigned to four stocking densities D1, D2, D3 and D4 being 10 birds/m2, 13 birds/m2, 16 birds/m2 and 19 birds/m2 in the first phase (0-6 weeks); 8 birds/m2, 11 birds/m2, 14 birds/m2 and 17 birds/m2 in the second phase (7-12 weeks) and lastly 6 birds/m2, 9 birds/m2, 12 birds/m2 and 15 birds/m2 in the third phase (13-18 weeks) due to slaughtering which occurred at the end of each phase in a completely randomized design. The number of replicates per treatment was four. Parameters recorded included feed intake, body weight (BW), body weight gain (BWG), feed conversion ratio (FCR), mortality, final live weight, dressed weight and dressing percentage. Data were analysed using the General Linear Model Procedures in Statistical Analysis System. The growth parameters of family chickens reared in three phases under intensive system were not significantly (P<0.05) affected by different stocking densities probably because of slaughtering that occurred at six weekly intervals. A significant stocking density and age interaction occurred for feed intake (P<0.0002) and (P<0.0001) in the first and third phases, respectively. These results indicate that the optimum stocking density for family chickens under intensive system of management may be 10 birds/m2for first phase, 8 birds/m2 for second phase and 9 birds/m2 for third phase. Introduction The significance of stocking density in broiler production (e.g., production performance, vitality and health condition of chickens) was established at the beginning of development of industrial poultry production (Skrbi et al., 2009a). As current recommended densities are rather variable it is critical if guidelines are to be established that they be based on sound science (Estevez, 2007). Stocking 291 Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 density has critical implications for the broiler industry because higher returns can be obtained as the number of birds per unit space increases. stocking densities on performance of family chickens up to 18 weeks of age under intensive system. Materials and Methods In broiler production, stocking density that is floor surface per chicken is a very important welfare factor which directly and indirectly influences and determines the level of growth of chicken body weight (BW) ( krbi et al., 2009b). Profitability can be realized by efficient management of floor space. Poultry producers tend to increase the number of birds per unit of space in order to reduce housing, equipment, and labour costs per unit of space. According to Estevez (2007), the negative consequences of high stocking density include reduced final BW, feed intake and FCR, and greater incidences of foot-pad dermatitis, scratches, bruising, poorer feathering and condemnations. Research consistently indicates that the health and welfare of broilers is compromised if space allowances drop below 0.0625 to 0.07 m2/bird (equivalent to 34 to 38 kg/m2) depending on final BW (Estevez, 2007). Location The experiment was carried out at Botswana College of Agriculture (BCA) Guinea Fowl Unit in Sebele at 24º 33 S, 24º 54 E at an altitude of 994 m above sea level (Aganga and Omphile, 2000).The study lasted for 18 weeks from April to August 2013. An open open-sided poultry house with concrete floors and roofed with corrugated iron sheets was used. Design of the study A total of 248 day old chicks were obtained from a local farmer in Gaborone and reared up to 18 weeks of age under intensive system. A completely randomized design (CRD) was employed in this experiment. Birds were randomly allocated to four stocking densities (treatments) with each treatment having four replicates. The stocking densities were D1 (10 birds/m2), D2 (13 birds/m2), D3 (16 birds/m2) and D4 (19 birds/m2) in the first phase (0-6 weeks). Thereafter, stocking density was reduced to D1 (8 birds/m2), D2 (11 birds/m2), D3 (14 birds/m2) and D4 (17 birds/m2) in the second phase (7-12 weeks). and further reduced to D1 (6 birds/m2), D2 (9 birds/m2), D3 (12 birds/m2) and D4 (15 birds/m2) in the third phase (13-18 weeks). due to slaughtering which occurred at the end of each phase. Each rearing phase lasted for six weeks. Stocking density D1 (10, 8 and 6 birds/m2) served as control. Stocking density selection was based on previous studies of Beg et al (2011) and Tayeb et al (2011) who found stocking The negative consequences of stocking density and the quest for profitability necessitate the evaluation of optimum density allowances for various species of poultry, especially family chickens. Family poultry encompasses the wide variety of small-scale poultry production systems found in rural and peri-urban areas of developing countries (FAO, 2014). Family chickens are usually kept in places of varying sizes in owners homes with some chickens being widely spaced while others are crowded. These varying stocking densities affect productivity of family chickens.Therefore, this study was carried out to investigate the effect of 292 Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 density of 10 birds/m2 is to be ideal for broilers in Bangladesh and Duhok region. recorded weekly in each replicate using an electronic balance to the nearest .01g throughout the experimental period. The difference between feed given and left over feeds was used to calculate feed intake (grams). Performance parameters measured included average feed intake, average BW, average BWG, FCR, mortality, dressed weight and dressing percentage. Data were calculated using the formulae given by Djakalia et al (2011): Experimental birds and their management Prior to the start of the experiment all chicks were individually weighed and randomly distributed to four groups of different stocking densities. Each pen was 1 m2. In each treatment, replicate birds were assigned identification numbers and then wing banded. The chicks were kept under deep litter management system in a house with windows for ventilation. Each pen was equipped with an electric brooder, feeder, drinker and an automatic drinker. Feed intake (FI) is the ratio between the total quantity of feed consumed (QFC) on a given period over the number of subjects fed (NSF) on the same period. Data collection was done following two weeks (0-2 weeks of age) of acclimatization of chicks to experimental diets. Data were collected from 3 to 18 weeks of age. Body weight (BW) is the ratio between total weight of birds (TWB) in a given flock and the number of birds (NB) of this flock: Experimental diets Birds were fed commercial broiler starter diet (0 to 6 weeks), commercial broiler grower diet (7 to 12 weeks) and commercial broiler finisher diet (13 to 18 weeks). Commercial broiler diets were obtained from retail shops in Gaborone. Feed and water were provided ad libitum throughout the experimental period. Birds in each replicate were group fed. Feed intake was measured by giving preweighed feed allocated to each replicate group throughout the week and then weighing back all the refusals at the end of the week. Pen body weights were also recorded weekly. Average weight gain (AWG) represents the difference between the average weight of the current week (AWc) and that of the previous week (AWp). It was determined using the formula, AWG = AWc Awp Feed conversion ratio was determined by dividing total feed intake by total BWG (Ratsaka et al., 2012). Mortality was recorded daily and calculated as the ratio between the number of the dying birds and the initial total number of birds in the flock multiplied by 100. Data collection procedure At 6, 12 and 18 weeks of age, 2 birds from Feeds fed to birds and refusals were 293 Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 each replicate were randomly selected and sacrificed by stunning at the BCA slaughter house and carcass weight determined using an electronic balance scale with accuracy 0.001 g. stocking densities for all phases feed intake was not significantly different. However, the highest feed intake was recorded in D1 (308.71 g) at week 4 in the first phase; in D1 (601.96 g) at week 8 in the second phase and in D1 (789.47 g) at week 18 in the third phase. Stocking density did not affect average feed intake because of slaughtering which was done at the end of each phase. In agreement Feddes et al (2002) found that birds reared at 11.9 birds/m2 stocking density consumed the least feed (2,993 g/bird) compared to those at 14.3 birds/m2 which consumed most feed (3,183 g/bird). These results are in disagreement with Iyasere et al (2012) who found that increased stocking density reduces feed intake. Similarly, Tong et al (2012) observed that under three stocking densities (12.5, 17.5 and 22.5 birds/m2) feed intake decreased significantly in each period as stocking density increased. On the contrary, Nahashon et al (2009) observed significantly higher feed intake in birds raised at stocking density of 10.7 birds/m2compared to 15.6, 13.6, and 12 birds/m2. In this study, significant stocking density and age interaction occurred for feed intake (P<0.0002) and (P<0.0001) in the first and third phases, respectively. The current results indicate that feed intake declined with increased stocking density. Anon (2013) attributed the decline in feed intake to restricted access to the feed, increased heat stress and increased ammonia level which occurs under heavily stocked birds. Also, the physical access to feeders is probably limited due to increased stocking density, as well as, the competition between birds to get to the feeder (Abudabos et al., 2013). Statistical analysis A General Linear Model (GLM) procedure of Statistical Analysis Systems SAS (9.1) Inc. (2002-2003) was used to estimate the differences between treatment means for different stocking densities. Dunnett s mean test was used to separate Least Square Means. Significance was declared at P<0.05. The following model was used: Statistical model: Y = + ijk + i + j + ( )ij ijk Where: yijk=Response variable from ith experimental unit (eu) with jth treatment µ = General mean effect i = ith Stocking density s effects on family chickens growth j=j th age effects on family chickens growth ( )ij= ijth stocking density and age interaction ijk = Error ijktheu ~N(0, 2 ) Results and Discussion Feed intake Feed intake in the second phase did not vary across the rearing period while it significantly increased with the rearing period in all densities in the first and third phases (Tables 1 to 3). Among the Body weight and body weight gain Body weight increased significantly (P<0.05) over time in all the phases 294 Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 (Tables1 to 3). However, BW in all phases was not affected by stocking density due to slaughtering which occurred at the end of each phase. The highest BW was recorded in D1 (409.75 g) at week 6 in the first phase; in D1 (1269.40 g) at week 12 in the second phase and in D2 (2033.20 g) at week 18 in the third phase. The result on BW is in agreement with Dozier et al. (2006) and Sekeroglu et al (2011) who found a significant effect of stocking density on BW of broilers. However, the current finding is in disagreement with ElDeek and Ai-Harthi (2004) and Tayeb et al (2011) who found no influence of stocking density on BW of broiler chicks. No stocking density x age interaction for BW in all the phases was found in this study indicating that the influence of stocking density did not vary for BW. In this study, stocking density of 9 birds/m2 showed better performance in achieving the final market weight because of high feed intake as access to the feed and water was not restricted. Generally, no significant difference was observed in BWG of chickens in all the phases (Tables 1 to 3). The highest BWG was recorded in D2 (164.17 g) at week 6 in the first phase; in D1 (1269.40 g) at week 10 in the second phase and in D2 (352.10 g) at week 18 in the third phase. stocking density and age interaction for BWG was observed in all the rearing phases in this study. Feed conversion ratio There was no variation observed in FCR for family chickens in all the phases (Tables 1 to 3). The highest FCR was recorded in D2 (4.63) at week 4 in the first phase; in D3 (2.49) at week 12 in the second phase and in D1 (3.84) at week 14 in the third phase. This indicates that chickens in the above stocking densities are poor converters of feeds to meat, probably because they have not been selected for faster growth rate. Nahashon et al (2009) observed significantly lower FCR in birds raised in floor densities of 13.6 and 12 birds/m2 than those raised on floor densities of 15.6 and 10.7 birds/m2. Similarly, Sekeroglu et al (2011) found that birds reared at 17 birds/m2 had better FCR than those reared at 9 and 13 birds/m2 groups at 21-42 days. The current results are in agreement with Sreehari and Sharma (2010) who reported that birds of lower density groups have a chance to consume more feed which is a waste because they cannot convert it into meat and are unable to show better FCR value. The authors also found that FCR between stocking density groups was not significant between 0-21 and 0-42 days but was significant at 21-42 days of age. In this study, a significant stocking density and age interaction occurred for FCR (P<0.0395) in the third phase indicating that the influence of stocking density varied for FCR during the third phase. The current result is consistent with Iyasere et al (2012) who reported that increased stocking density reduces BWG of the birds. Sekeroglu et al (2011) raised Ross 308 broilers under three stocking densities (9, 13 and 17 birds/m2) and observed that BWG at density of 13 birds/m2 was higher than that of other two stocking densities during the 2nd and 3rd weeks of age. The authors also found that birds reared at stocking density of 17 birds/m2 had the lowest BWG of all the three groups (9, 13 and 17 birds/m2) during the 4th, 5th and 6th weeks. No Mortality Stocking density did not affect mortality. In the first phase mortality occurred in D1 at week 4 and 6 (0.22±0.11 % each) and D2 at week 6 (0.33±0.11 %). 295 Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 Table.1 Means and standard errors of growth parameters of family chickens reared up to 6 weeks of age under intensive system Parameter Feed intake (g) Density Age (weeks) 10 13 3 4 6 SE 79.58ax 109.07a 308.71ay 236.29by 185.97az 222.98ab 14.12 Significance of effect (P) Density Age Intera ction 0.3175 0.0001 0.0002 10.59 0.0727 0.0001 0.1940 8.017182 14.87 0.4016 0.0001 0.2108 25.3701 8 0.58 0.3224 0.0001 0.2601 x 16 19 Body weight (g) 10 CV 14.46373 y ax by 99.43 121.84a 221.61 262.70ab 252.86by 241.30ab x y y 153.78a 269.03ay 409.75az 227.33ay 391.49az 243.36ay 395.60az 235.63ay 369.48az x 13 153.57a x 16 156.78a x 19 164.32a x Body Weight Gain (g) FCR 10 - 115.25ax 140.73ax 13 16 19 - 73.76ax 86.58ax 71.32ax 164.17ay 152.24ay 133.85ay 10 13 16 19 - 2.83ax 4.63ax 2.60ax 3.71ax 1.36ax 1.39ay 1.67ax 1.83ay ab 46.69058 Means in the same column within a parameter with different superscripts differ significantly; xy Means in the same row within a parameter with different superscripts differ significantly P<0.05. FCR=Feed Conversion Ratio; CV= Cumulative Frequency and SE= Standard error. 296 Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 Table.2 Means and standard errors of growth parameters of family chickens reared from 8 to 12 weeks of age under intensive system Parameter Feed intake (g) Density Age (weeks) 8 11 8 10 601.96a 564.66a x x 470.60b 502.89a x x b 14 17 Body weight (g) 8 11 14 17 Body Weight Gain (g) 8 11 14 17 FCR 8 12 34.55 553.65ax 513.05ax 471.37 553.44a x x 459.96b 584.04a x x 545.74ax 674.27a 999.35a 1269.40 x y az 639.06a 936.95a 1195.48 x y az 645.80 a x 634.27 958.41 a y a 955.17 SE Significance of effect (P) Densi Interactio Age ty n 0.064 0.1162 0.3150 9 CV 13.0319 7 541.02ax 37.66 0.204 6 0.0001 0.9868 8.014930 26.43 0.684 2 0.0016 0.9441 19.41427 0.21 0.628 1 0.0159 0.3668 20.35758 1178.71 az a 1189.77 x y az 264.52a 325.08a x x 247.56a 297.90a x x 250.20a 312.61a x x 264.79a 320.90a x x 234.60ax 2.41ax 1.76ax 2.06ax 270.05ax 258.51ax 220.30ax 11 1.96ax 1.71ax 2.02ax 14 1.95ax 1.85ax 2.49ax 17 1.84ax 1.83ax 2.34ax ab Means in the same column within a parameter with different superscripts differ significantly; xy Means in the same row within a parameter with different superscripts differ significantly;P<0.05. FCR=Feed Conversion Ratio; CV= Cumulative Frequency and SE= Standard error. 297 Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 Table.3 Means and standard errors of growth parameters of family chickens reared from 14 to 18 weeks of age under intensive system Parameter Density Age (weeks) 14 Feed intake (g) Body weight (g) Body Weight Gain (g) FCR 6 690.47ax 9 647.55ax 16 Significance of effect (P) 18 435.63a y 789.47az 525.08a 614.37bx y y 431.09 a 12 434.77bxy 15 452.32bx 6 1476.75ax 1708.4 9axy 1920.07 9 1428.25ax 2033.20 12 1452.72ax 15 1487.70ax 1681.1 0axy 1643.6 4axy 1675.3 6axy 6 207.35ax 9 232.78ax 12 274.02ax 15 297.93ax 6 3.84ax x 429.55a x 231.75a x 252.85a x 190.92a x 187.66a SE 27.34 Densit y 0.000 1 Interact ion 0.000 00.0001 1 Age CV 9.89565 9 543.34by 637.79by ay 76.76 0.741 6 44.34 0.318 8 0.137 1 0.8449 9.10021 3 0.461 5 0.2587 37.2366 6 0.038 3 0.0395 38.1773 8 0.000 1 az 1848.69 ay 1889.14 ay 211.58ax 352.10bx 205.05ax x 213.77ax 1.93ax 3.87ax 0.50 9 2.82abx 2.09ax 2.24ax 12 1.60bx 2.40ax 3.25ax 15 1.56bx 2.53ax 3.20ax ab Means in the same column within a parameter with different superscripts differ significantly; xy Means in the same row within a parameter with different superscripts differ significantly;P<0.05. FCR=Feed Conversion Ratio; CV= Cumulative Frequency and SE= Standard error. 298 Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 Table.4 Mean carcass characteristics and live weight of family chickens slaughtered at 6 weeks Parameter Live weight (g) Dressed weight (g) Density Age (weeks) 6 Significance of effect (P) 496.63a 463.88a 483.25a 465.50a 10 13 16 19 10 285.75a SE Treatment CV 27.33 0.8073 11.45082 18.24 0.8164 268.75a 284.63a 266.50a 57.50a 0.81 0.4933 Dressing (%) 57.75a 16 58.88a 19 57.15a ab Means in the same column within a parameter with different superscripts differ significantly;P<0.05.; CV= Cumulative Frequency and SE= Standard error. 13 16 19 10 13 13.20156 2.799843 Table.5 Mean carcass characteristics and live weight of family chickens laughtered at 12 weeks Parameter Density Age (weeks) 12 Significance of effect (P) SE Treatment CV Live weight (g) 8 11 14 17 1594.63a 1656.38a 1598.88a 1524.50a 76.28 0.6887 9.573201 Dressed weight (g) 8 11 14 17 8 974.05a 913.525a 1079.38a 1040.68a 61.15a 55.80a 52.15 0.3878 10.40969 2.95 0.0350 9.322359 Dressing (%) 11 14 67.55b 17 68.28b ab Means in the same column within a parameter with different superscripts differ significantly;P<0.05.; CV= Cumulative Frequency and SE= Standard error. 299 Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 Table 6: Mean carcass characteristics and live weight of family chickens slaughtered at 18 weeks Parameter Density Age (weeks) Significance of effect (P) 18 SE Treatment CV Live weight (g) 6 9 12 15 2319.38a 2540.25a 2436.88a 2351.25a 81.75 0.2739 6.778734 Dressed weight (g) 6 9 1605.13a 1765.75a 72.88 0.4094 8.656268 12 1725.13a 15 1639.25a 69.20a 1.07 0.7437 69.43a 70.75a 69.63a ab Means in the same column within a parameter with different superscripts differ significantly;P<0.05.; CV= Cumulative Frequency and SE= Standard error. 3.057189 Dressing (%) 6 9 12 15 The highest mortality was recorded in the third phase at week 16 in stocking density D4 at week 16 (0.52±0.18 %). This may be overcrowded. This finding on mortality is consistent with Feddes et al (2002) who found that stocking density had no effect on mortality. In agreement with Beg et al (2011) no significant (P<0.4100) stocking density and age interaction was observed for mortality in this study. due to pecking which occurred as birds were birds under stocking density D3 (12 birds/m2) was significantly higher under four stocking densities (8, 10, 12 and 14 bird/m2) at 6 weeks of age. In contrast, Tayeb et al (2011) found no significant effect of stocking density on carcass weight under three stocking densities (8.66, 10.41 and 13.36 birds/m²). Carcass characteristics and live weight In this study, the highest dressed weight was recorded in D3 (2496.63 g) at week 6 in the first phase; in D3 (1079.38 g) at week 12 in the second phase and in D2 (1765.75 g) at week 18 in the third phase. In contrast, Beg et al (2011) found that dressing percentage of birds on D1 (8 birds/m2) and D2 (10 birds/m2) stocking densities was significantly (P<0.05) higher compared to D3 (12 birds/m2) and D4 (14 birds/m2) stocking densities. The authors All carcass characteristics were not affected by stocking density in all the phases (Tables 4 to 6). The highest live weight was recorded in D1 (496.63 g) at week 6 in the first phase; in D2 (1656.38 g) at week 12 in the second phase and in D2 (2540.25 g) at week 18 in the third phase. The current result is in contrast to Beg et al (2011) who reported that the average live weight of Cobb-500 broiler 300 Int.J.Curr.Microbiol.App.Sci (2014) 3(3): 291-302 concluded that lower stocking density resulted in higher dressing percentage. growth for broilers and higher yield of processed carcass. Also, low stocking density allows for better body development and carcass conformation (Skrbic et al., 2009a).The highest dressing percentage was recorded in D3 (58.88 %) at week 6 in the first phase; in D4 (68.28 %) at week 12 in the second phase and in D3 (70.75 %) at week 18 in the third phase. The present results are in disagreement with Sekeroglu et al (2011) who reported no influence of stocking density on carcass yield. Similarly, Tayeb et al (2011) reported no significant effect of different stocking density on carcass weight and dressing percentage of broiler chickens. According to Skrbic et al. (2008), low stocking density provides more intensive Botswana.Government Printers, Gaborone. Beg, M.A.H., Baqui, M.A., Sarker, N.R. and Hossain, M.M. 2011. Effect of Stocking Density and Feeding Regime on Performance of Broiler Chicken in Summer Season. International Journal of Poultry Science,10 (5): 365-375. 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International Journal of Poultry Science, 3: 635-645. Estevez, I. 2007. Density allowances for broilers: where to set the limits? Poultry Science, 86: 1265 1272. FAO, 2014. Family poultry development Issues, opportunities and constraints. Animal Production and Health working paper No. 12. Rome. The growth parameters of family chickens reared under intensive system were not significantly affected by the different stocking densities probably due to the slaughtering that occurred at 6, 12 and 18 weeks of age. Stocking density of 10 birds/m2 for the first phase (1-6 weeks), 8 birds/m2 for the second phase (7-12 weeks) and 9 birds/m2 for the third phase (13-18 weeks) had better performance for almost all growth parameters. The current results suggest that the optimum stocking density for family chickens reared under intensive system may be 10 birds/m2 for the first phase, 8 birds/m2 for the second phase and 9 birds/m2 for the third phase. References Anon., 2013. 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