histology data
Note: Field of view of frames = 0.84mm x 0.84 mm (! some pics are composed of two frames)
*note: GFAP stain = red, Neun = green. Both red and green fluorescence photographs were taken.
* contours of GFAP and NeuN stains were almost identical
*Different resorbable materials (chitosan, oxidised cellulose) were tried as such, without electrodes inside.
*Also, the dummy versions of the implants themselves with porous chitosan reinforcing layer were put in.
*A control was present as well, i.e. only removing meninges without putting resorbable material or electrode in
* control was at contralateral side of implant
*Depth of cavity was measured (with the help of CorelDraw graphics software)
>There is significant variation between the experiments (manual action of surgery,…).
>Also, the control group (only meninges removed, no implant ) did not give significantly better results as can clearly be seen in figure 11.
>Thus, it was not possible to draw conclusions on which material behaved better,
> It can be concluded that the operation procedure is the major factor influencing tissue damage.
resorbable material put in
name of material + rat (FCx)
Chitosan FC11
Chitosan FC15
chitosan FC16
Surgicel FC12
Burr hole made and meninges removed, implant put in
Control (contralateral side)
Only burr hole made and meninges removed, no implant
Surgicel FC 13
Surgicel FC14
For a second group another microscope had to be used. The field of view is now 4mm x 4mm
RH: burr hole Right Hind position, LF: Left Front position,…
Code for rat_slice number
FC6_26
RH:chitosan
FC6_26
LH: net electrode
remark: a lot of damage compared with the rest (also compared with identical exp.)
FC6_15_links
FC6_15_rechts_stitch
LF: star shaped elektr.
RF: bore hole + meninges removed
FC7_3_links
LF: net elektrode
FC7_3_rechts RF: bore hole + meninges removed
FC7_31_links_stitch
LH: chitosan
FC7_31_rechts
RH: net electrode
FC8_5_links
RF: no action (control)
FC8_5_links
LF: bore hole+meninges removed
measured depth of cavity [microns]
chitosan
FC11
FC15
FC16
FC7_31 LH
FC6 RH
only burr hole
315
247
360
400
151
151
435
412
327
350
390
321
198
264
surgicel
FC12
FC13
FC14
elektrode
FC6 LH
FC6 LF
FC6_15_RF
FC7_3_LF
FC7_3_RF
FC7_31_RH
FC8_5_LF
650
277
326
405
522
352
400
mean (all implants)
mean (resorbable sheet only)
std
318
83
mean(full dummy implant)
std
421
161
mean(surgicel)
std
356
28
mean(chitosan sheet)
std
331
66
mean(burr hole only)
std
339
112