Supporting Materials
Journal name: Applied Microbiology and Biotechnology
Manuscript title: Profiling microbial community structures across six large oilfields in
China and the potential role of dominant microorganisms in bioremediation
Author names: Weimin Sunab, Jiwei Lia*, Lei Jianga, Zhilei Sunc, Meiyan Fud, Xiaotong Penga*
a
Sanya Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya,
572000,China.
b
Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, 08901,
NJ, U.S.
c
Qingdao Institute of Marine Geology, Qingdao, 266071, China
d
College of Energy Resources, Chengdu University of Technology, Chengdu, 610059, China
*Corresponding author. Tel./fax: +86-0898-88380102; E-mail address:
[email protected] ( J. Li)
Tel./fax: +86-0898-88380110; E-mail address: [email protected] (X.Peng)
Table S1 Geographical and contamination information for the 24 soils taken from six oilfields.
Sample
Elevation
Contamination
Oilfield
Climate regions
DQ1
151
oil contaminated
Daqing oilfield
temperate continental monsoon climate
DQ2
151
oil contaminated
Daqing oilfield
temperate continental monsoon climate
DQ3
151
oil contaminated
Daqing oilfield
temperate continental monsoon climate
DQ4
151
Uncontaminated
Daqing oilfield
temperate continental monsoon climate
CQ1
1218
oil contaminated
Changqing oilfield
temperate continental monsoon climate
CQ2
1218
oil contaminated
Changqing oilfield
temperate continental monsoon climate
CQ3
1218
oil contaminated
Changqing oilfield
temperate continental monsoon climate
CQ4
1218
oil contaminated
Changqing oilfield
temperate continental monsoon climate
CQ5
1218
Uncontaminated
Changqing oilfield
temperate continental monsoon climate
DY1
11
oil contaminated
Dongying oilfield
warm temperate continental semi-humid monsoon climate
DY2
11
oil contaminated
Dongying oilfield
warm temperate continental semi-humid monsoon climate
DY3
11
oil contaminated
Dongying oilfield
warm temperate continental semi-humid monsoon climate
DY4
11
Uncontaminated
Dongying oilfield
warm temperate continental semi-humid monsoon climate
JS1
11
oil contaminated
Jiangsu oilfield
subtropical humid monsoon climate
JS2
11
oil contaminated
Jiangsu oilfield
subtropical humid monsoon climate
JS3
11
oil contaminated
Jiangsu oilfield
subtropical humid monsoon climate
JS4
11
Uncontaminated
Jiangsu oilfield
subtropical humid monsoon climate
XJ6
946
oil contaminated
Xinjiang oilfield
temperate continental arid climate
XJ7
946
oil contaminated
Xinjiang oilfield
temperate continental arid climate
XJ12
946
oil contaminated
Xinjiang oilfield
temperate continental arid climate
JL1
130
oil contaminated
Jilin oilfield
temperate continental monsoon climate
JL2
130
oil contaminated
Jilin oilfield
temperate continental monsoon climate
JL3
130
oil contaminated
Jilin oilfield
temperate continental monsoon climate
JL4
130
Uncontaminated
Jilin oilfield
temperate continental monsoon climate
Table S2 Phyla with relative abundances >5% in at least one sample and their relative abundances (%) in tested soil samples.
Phylum
CQ1
CQ2
Actinobacteria
55.5
11.6
Proteobacteria
22.8
9.3
Acidobacteria
2.7
1.9
Verrucomicrobia
0.8
Planctomycetes
Firmicutes
CQ3
CQ4
CQ5
DQ1
DQ2
8.4
9.2
76.4
8.8
7.6
13.4
14.5
8.9
19.8
15.9
23.3
1.5
1.8
26.9
27.2
0.7
8.6
0.9
0.3
5.1
1.0
0.8
3.7
1.5
0.6
5.5
1.8
2.7
11.6
5.8
Crenarchaeota
0.4
0.1
2.4
0.2
Chloroflexi
1.2
0.7
1.1
Bacteroidetes
0.9
0.9
Euryarchaeota
0.1
0.1
DQ3
DQ4
DY1
DY2
DY3
DY4
4.0
7.6
10.0
5.5
7.3
9.3
34.9
15.4
19.9
25.2
10.8
12.7
15.5
19.3
9.1
19.3
23.6
24.7
6.0
3.1
4.1
3.1
4.8
6.4
11.1
5.0
2.1
2.8
6.2
11.3
2.8
2.2
3.5
2.2
2.5
2.9
0.3
0.9
3.9
2.5
5.0
2.8
0.5
0.4
0.8
0.7
4.1
1.5
4.0
5.1
0.4
2.5
3.6
6.2
0.3
46.4
0.0
0.4
0.4
1.4
JL1
JL2
JL3
JL4
JS1
JS2
JS3
7.0
7.0
23.0
23.6
2.7
19.2
5.0
11.0
11.1
18.2
20.9
7.3
11.2
59.0
23.6
27.8
17.0
14.4
0.9
2.4
11.6
5.8
5.5
3.2
4.4
0.5
2.8
2.6
4.6
3.4
4.5
4.0
2.2
1.9
2.1
2.0
3.2
2.4
2.0
5.8
5.7
6.5
7.8
3.4
2.5
0.8
0.5
0.5
1.4
0.5
3.2
1.5
3.5
4.1
3.8
4.6
1.0
0.7
0.4
0.2
0.1
0.5
JS4
XJ12
XJ6
XJ7
29.3
9.5
28.2
23.7
12.6
60.9
8.6
11.4
1.4
6.1
2.6
5.4
3.7
0.4
0.6
1.8
0.6
1.1
1.2
1.2
1.0
1.7
2.0
1.4
2.0
1.3
5.6
10.1
4.5
4.6
4.7
19.3
2.8
2.1
0.3
0.2
0.2
8.4
0.7
3.9
2.8
2.9
1.9
0.7
3.2
0.9
1.4
1.3
0.8
1.0
2.0
1.4
1.4
2.5
4.5
5.5
1.2
4.1
0.6
1.1
0.3
0.3
0.2
70.7
36.0
11.0
0.4
5.0
0.2
0.1
Table S3 Dominant genera and their relative abundances (%) in tested soil samples.
Taxon
CQ
1
CQ
2
CQ
3
CQ
4
CQ
5
DQ
1
DQ
2
DQ
3
DQ
4
DY
1
DY
2
DY
3
Arthrobacter
17.5
0.2
0.7
0.3
6.0
1.1
0.7
0.4
0.5
0.3
0.8
0.6
Dietzia
11.4
0.2
0.2
0.3
56.3
0.3
0.2
0.3
0.2
0.4
0.2
Pseudomonas
8.1
0.1
0.3
0.3
2.6
0.2
0.3
1.3
0.2
0.2
Rhodococcus
5.6
0.2
0.1
0.1
1.5
0.1
0.1
0.1
1.1
0.2
Nocardioides
3.3
0.3
0.2
0.2
1.3
0.4
0.3
0.1
0.2
Planomicrobium
1.6
0.1
0.1
3.8
1.5
0.1
0.1
0.1
Thermotoga
1.5
0.0
0.0
0.0
0.4
0.0
0.0
Citrobacter
1.5
0.0
0.0
0.0
0.5
0.0
0.0
Acinetobacter
1.0
0.0
0.3
0.0
0.2
0.0
Alicyclobacillus
0.9
0.5
0.7
0.6
0.6
Mycobacterium
0.7
3.0
0.3
0.1
0.6
Streptomyces
0.4
0.5
0.4
0.0
Marinobacter
0.3
0.1
0.3
Skermanella
0.3
0.1
Halomonas
0.2
0.1
Singulisphaera
0.2
Alkanindiges
DY
4
JL
1
JL
2
1.6
0.9
0.7
2.4
0.2
0.2
0.3
0.2
0.6
0.2
0.1
0.1
0.1
0.1
0.1
0.2
0.1
0.2
0.1
0.2
0.1
0.1
0.1
0.1
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.2
0.0
0.0
0.7
0.6
0.9
0.8
0.2
0.2
0.2
0.2
0.6
0.1
0.1
0.0
0.2
0.2
0.2
0.1
0.1
0.1
0.1
0.1
0.1
2.6
0.4
0.1
0.1
1.0
0.3
0.1
0.2
0.1
0.1
0.1
Sphingomonas
0.2
0.1
0.2
Opitutus
0.2
0.1
0.9
Truepera
0.2
0.1
Solirubrobacter
0.1
Pirellula
Psychrobacter
JL3
JL
4
XJ1
2
XJ6
XJ
7
JS1
JS2
JS3
JS4
1.8
0.1
0.9
0.2
7.6
0.3
5.3
4.9
0.9
0.7
0.1
0.4
0.9
4.8
4.4
0.9
1.6
0.1
0.4
0.7
0.1
0.3
2.5
1.0
4.1
0.3
0.5
0.1
2.3
5.5
0.0
0.1
0.4
0.6
0.2
0.3
0.6
0.2
0.2
1.6
1.0
0.0
0.2
0.1
1.2
0.1
2.8
0.7
0.1
0.1
0.1
0.2
0.1
0.4
4.5
1.4
0.5
0.4
0.2
0.2
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.2
0.0
0.0
0.0
0.1
0.0
0.0
0.7
0.7
0.6
0.6
0.8
0.5
0.8
0.8
0.4
0.6
0.8
0.7
0.9
0.1
14.
9
2.6
0.1
0.1
0.2
0.1
0.2
1.4
2.3
0.1
0.2
0.2
0.5
0.7
0.4
2.5
0.0
0.1
0.1
0.1
0.1
0.1
0.1
0.2
0.2
0.0
0.1
0.2
0.1
0.2
0.6
0.2
0.1
0.3
0.4
0.1
0.1
0.2
0.2
0.2
0.2
0.2
0.2
0.0
19.
2
0.2
20.0
0.1
0.1
0.3
0.2
0.5
0.1
0.2
0.1
0.3
0.3
0.1
0.7
1.1
0.1
1.9
0.1
0.4
0.1
0.3
0.3
0.1
0.2
0.1
0.2
0.4
0.1
0.1
0.1
0.1
0.1
0.1
1.2
0.4
5.1
0.1
6.2
0.1
0.1
0.3
0.2
0.2
0.2
0.2
0.2
0.7
0.4
0.3
0.6
0.3
0.3
0.1
0.1
0.1
0.5
0.1
0.5
0.3
0.2
0.1
0.1
0.2
1.0
0.1
0.1
0.1
0.1
0.1
0.1
0.3
2.5
0.0
0.1
0.5
0.1
0.2
0.1
0.3
0.3
0.1
0.7
1.3
0.2
1.5
0.2
0.5
0.8
1.5
1.0
0.8
0.4
0.6
0.1
0.6
0.1
0.2
0.2
0.2
0.3
0.1
0.0
1.2
1.1
1.4
0.9
0.7
0.6
0.7
0.5
1.6
0.6
0.6
1.2
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.0
1.0
0.0
0.1
0.0
0.1
0.1
0.1
0.1
0.1
0.0
0.0
0.0
0.1
0.2
1.8
0.6
0.9
0.2
0.3
0.1
0.1
0.1
0.2
0.0
0.1
0.2
0.3
0.0
0.1
0.1
0.1
0.5
0.3
0.2
0.3
0.4
0.3
0.0
0.4
0.0
0.7
0.0
0.9
0.3
0.1
0.1
0.2
0.0
0.0
1.3
0.5
0.1
0.3
0.2
1.1
0.2
0.2
1.0
0.4
0.5
0.4
0.0
0.0
0.0
0.2
0.1
0.2
0.1
0.1
0.1
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
1.1
0.0
0.0
Gemmatimonas
0.1
0.1
0.9
0.0
0.1
0.3
0.3
0.5
0.9
0.1
0.3
1.0
0.7
0.7
1.0
0.3
0.3
0.0
0.1
0.1
0.1
0.1
0.1
0.1
Lysobacter
0.1
0.0
0.2
0.2
0.0
0.1
0.1
1.1
0.0
0.0
0.2
0.0
0.1
0.0
0.1
0.1
0.0
0.0
0.3
0.0
0.0
0.1
0.0
0.1
Planctomyces
0.1
0.0
0.2
0.1
0.0
2.6
0.5
0.2
0.3
1.2
1.4
0.1
0.2
0.3
0.3
0.4
0.6
0.2
0.1
0.5
0.2
0.3
0.2
0.1
Luteolibacter
0.1
0.0
0.1
0.0
0.0
0.3
0.2
0.4
0.1
0.1
1.7
0.0
0.1
0.3
0.1
0.1
0.1
0.0
0.0
0.0
0.0
0.0
0.0
0.1
0.7
Polaromonas
0.0
0.0
0.0
0.0
0.0
0.0
0.1
1.2
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
Idiomarina
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.1
0.0
2.3
0.0
0.0
Alcanivorax
0.0
0.0
0.0
0.0
0.0
0.1
0.0
0.0
0.0
0.1
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.2
0.0
4.7
0.0
0.0
Thiobacillus
0.0
0.0
0.0
0.0
0.0
0.0
0.0
4.8
0.0
0.2
0.0
0.0
0.0
0.0
0.0
0.1
0.0
0.0
0.0
0.0
0.0
0.1
0.0
0.0
Methylobacter
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.8
0.0
0.2
0.0
0.0
0.0
0.0
0.0
0.1
0.0
0.0
0.0
0.0
0.0
0.0
0.0
Halalkalicoccus
0.0
0.0
0.0
10.8
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
17.
0
3.8
0.2
0.0
0.1
0.0
0.0
Natronomonas
0.0
0.0
0.0
4.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
7.7
2.6
0.4
0.0
0.1
0.0
0.0
Halosarcina
0.0
0.0
0.0
1.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
4.3
0.2
0.0
0.8
0.0
0.0
Haloterrigena
0.0
0.0
0.0
3.9
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
3.4
0.9
10.
9
0.1
0.0
0.1
0.0
0.0
Natronococcus
0.0
0.0
0.0
1.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.1
0.9
0.0
0.0
0.0
0.0
0.0
Figure S1 Pearson correlation between soil moisture and phylotype richness in 24 soil samples.