A
B
a
b
Prebleach
0.5 s
Protein
t ½ (s)
5s
C
GFP-NSD3S
D
Fluorescence Recovery
after 30 s (%)
8.4 s ± 1.9
# cells
79.9 % ± 5.7
35
E
GFP-NSD3S – Serum starvation
GFP-NSD3S – Nocodazole treatment
6
6
5
t1/2
t1/2
20
s
18
s
16
s
14
s
0s
20
s
18
s
16
s
14
s
12
s
10
s
8s
6s
0
4s
1
0
2s
1
12
s
2
8s
2
3
10
s
3
4
6s
4
4s
5
2s
Number of cells
7
0s
Number of cells
10 s