A
B
GFP-HP1β – Nocodazole treatment
12
H
d
10
Number of cells
a
G
8
6
G
4
GFP-HP1γ – Serum starvation
GFP-HP1α – Serum starvation
t1/2
t1/2
C
GFP-HP1β
n
Co
tr
ol
GFP-HP1γ
GFP-HP1α
e
e
e
ol
ol
ol
n
n
az nt
az nt
az nt
l
m
l
m
m on
o
o
d
d
d
i
i
e
o
e
e
u
r at
ru at
r u a ti
tr
co tm
tro oco tm
co tm
e
e
e
v
v
n
o
n
o
S ar
S ar
S arv
N rea
N rea
N rea
Co
Co
t
t
t
st
st
st
GFP-HP1
Endogenous
HP1
α-HP1β
α-HP1γ
α-HP1α
20
s
20
s
18
s
18
s
16
s
14
s
12
s
10
s
1
0
0s
20
s
18
s
16
s
14
s
12
s
0
8s
1
H
G
3
2
4s
G
2
5
4
6s
Number of cells
4
3
f
7
6
20
s
H
2s
c
8
5
10
s
14
s
16
s
GFP-HP1α – Nocodazole treatment
6
8s
12
s
10
s
6s
8s
t1/2
7
6s
H
G
t1/2
4s
18
s
e
4s
0s
20
s
18
s
16
s
14
s
12
s
8s
10
s
6s
4s
10
9
8
7
6
5
4
3
2
1
0
2s
Number of cells
H
G
2s
16
s
GFP-HP1γ – Nocodazole treatment
b
2s
Number of cells
0s
14
s
t1/2
9
8
7
6
5
4
3
2
1
0
Number of cells
12
s
10
s
6s
8s
4s
2s
0s
20
s
18
s
16
s
14
s
12
s
8s
10
s
6s
4s
0
t1/2
0s
H
2
2s
0s
Number of cells
GFP-HP1β – Serum starvation
8
7
6
5
4
3
2
1
0