B
A
L9
GF
Hoechst
GF
PHP
1
L9
29 α
GFP-HP1α
PHP
1β
29
GFP-HP1γ
GF
PHP
1γ
L9
29
GFP-HP1β
α-HP1γ
α-HP1α
GFP-HP1
Endogenous
HP1
α-HP1β
GFP
Prebleach
0.5 s
5s
10 s
C
GFP-HP1β
GFP-HP1γ
GFP-HP1α
t ½ (s)
GFP-HP1β
8.1 s ± 2.1
82.4% ± 7.8
43
GFP-HP1γ
8.8 s ± 1.9
75.6% ± 7.8
45
GFP-HP1α
9.5 s ± 1.9
69.6% ± 4.6
50
GFP-HP1β
Number of cells
7
6
5
4
3
2
t1/2
20
s
16
s
18
s
12
s
t1/2
14
s
6s
8s
4s
2s
0s
20
s
18
s
16
s
14
s
12
s
8s
1
0
6s
18
s
20
s
16
s
12
s
14
s
8s
10
s
6s
2s
0
4s
2
2s
Number of cells
4
GFP-HP1α
8
16
14
12
10
8
6
4
2
0
0s
6
4s
Number of cells
8
0s
# cells
GFP-HP1γ
10
t1/2
Fluorescence Recovery
after 30 s (%)
10
s
E
Protein
10
s
D