Supplementary materials
Table1. Primers used for RT-PCR and sequencing of the human coxsackievirus A24 strain K282/YN/CHN/2013 genome
Name
CA241F
CA241r
CA242R
CA244f
CA244F
CA246F
CA24 5r
CA244R
CA244r
CA248R
CA244ri
CA248r
CA241af
CA241ar
CA241arr
222
224
Sequence(5’→ 3’)
TTAAAACAGCTCTGGGGTTG
TGATGTTTCTAAGTCCGT
CTAGTCTGGTAAAAGCATGT
CCTTGGCAGTGGATTAAG
TCCACCTTGGAATCTTTCTT
GCACTGCAGTTAATGGATA
GAACTGTATGTAATTGTT
GACATTGTGTACACTT
GAACCGTTCCCTCCAACA
CTCCGAATTAAAGAAAAATT
GATTGTCATTGCTCTATT
CCAGTCATGCCAATGAA
AGATTGTTGTCATAAAGC
GCTTTATGACAACAATCT
AGTTGATCCTCCAGTTGC
CICCIGGIGGIAYRWACAT
GCIATGYTIGGIACICAYRT
Position
1-20
1748-1731
2352-2333
4085-4102
5006-5024
4459-4441
6751-6734
5961-5944
7459-7441
800-820
2969-2951
2270-2251
Note:F or f (forward) and R or r (reverse) mean the orientation of primer;-: indetermination
Table2. Nucleotide and amino acid identity between Joseph and EH24 and Other CVA 24 and K282/YN/CHN/2013 in all sequenced
genomic regions
Genomic region
5’UTR
VP4
VP2
VP3
VP1
2A
2B
2C
3A
3B
3C
3D
3’UTR
genome
Joseph(CVA24 prototype)
%Nucleotide
%Amino acid
identity
identity
88.5
79.2
100
77.9
96.3
79.4
95.4
78.9
93.1
78.9
92.0
76.3
90.7
78.6
94.8
76.2
92.0
77.3
86.4
80.5
97.3
84.5
98.0
97.1
80.0
95.3
EH24(CVA24v prototype)
%Nucleotide
%Amino acid
identity
identity
83.1
75.8
98.6
79.8
95.6
77.9
95.4
77.7
91.8
76.7
88.7
75.9
92.8
79.7
92.7
74.3
85.1
71.2
86.4
83.2
98.4
84.5
98.3
97.1
79.9
94.3
Other CVA 24
%Nucleotide
%Amino acid
identity
identity
84.1-87.1
74.4-80.2
95.7-100
76.5-80.8
94.5-97.4
77.4-79.6
94.6-97.1
76.4-79.3
91.5-93.1
74.9-78.7
85.3-92.7
75.9-79.7
88.0-93.8
78.9-81.1
92.7-94.2
75.1-78.5
83.9-93.1
72.7-77.3
81.8-90.9
79.2-82.7
94.0-96.7
83.9-85.8
97.0-98.9
89.9-100
79.5-82.1
93.3-96.9
Fig1. SimPlot and Bootscan analysis of entire genome sequence of K282/YN/CHN/2013 with the ten HEV-C strains.