Imaging of brain TSPO expression in a mouse model of amyotrophic lateral sclerosis
with 18F-DPA-714 and micro-PET/CT.
European Journal of Nuclear Medicine and Molecular Imaging
S. Gargiulo1,2§, S. Anzilotti3§, A.R.D. Coda1, M. Gramanzini1,2 , A. Greco4,2, M. Panico1,
A. Vinciguerra5, A. Zannetti1, C. Vicidomini1, F. Dollé6, G. Pignataro5, M. Quarantelli1,
L. Annunziato5,3, A. Brunetti4,2, M. Salvatore3, S. Pappatà 1*
1
Institute of Biostructure and Bioimaging, National Research Council, Via T. De Amicis 95, 80145, Naples,
Italy
2
Ceinge Biotecnologie Avanzate s. c. a r. l., Via G. Salvatore 486, 80145, Naples, Italy
3
IRCCS SDN, Via E. Gianturco 113, 80143, Naples, Italy
4
Department of Advanced Biomedical Sciences, University “Federico II”, Via S. Pansini 5, 80131, Naples, Italy
5
Division of Pharmacology, Department of Neuroscience, Reproductive and Dentistry Sciences, School of
Medicine, University “Federico II”, Via S. Pansini 5, 80131, Naples, Italy
6
CEA, Institute for Biomedical Imaging, 4 Place du Général Leclerc, 91401, Orsay, France
* Corresponding Author: Sabina Pappatà, [email protected]; Tel.: + 39 0812203414 / +39
3666115326; fax: +39 0812203498
§
These authors contributed equally
Supplementary Figures and Figure Legends
40
35
weight (g)
30
25
20
SOD1G93A
15
WT SOD1
10
5
0
7
8 10 11 12 13 14 15 16 17 18 19
age (weeks)
Supplementary Fig. 1 Body weight (mean±SD) registered from 7 to 19 weeks of age in
SOD1G93A and WT SOD1 mice
Supplementary Fig. 2 Representative confocal laser scanning microscopy images of doublelabel immunofluorescence for TSPO and microglia in the frontal association cortex in a
symptomatic SOD1G93A mouse (125-day-old) with CS of 4 and in a WT SOD1 control (130days-old). TSPO immunolabeling (visualized in red-cy3 immunofluorescence), Iba1
immunolabeling (visualized in green-cy2 immunofluorescence) and merged images
(TSPO+Iba1 visualized in orange). The images show the absence of TSPO and Iba1
immunoreactivities both in symptomatic SOD1G93A mouse and WT SOD1 control. Scale bar:
20 µm