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Figure S1.
IR
Tpase
IR
ligation
NdeI
epop
cat
NdeI
4490 bp
epop
KpnI
ligation
p15A-epop-cat
2470 bp
p15A
KpnI
KpnI
NdeI
Mx8
attp
ligation
Nde Ⅰ digestion with ends blunted
p15A
pMx8-epop
cat
5462 bp
epop
PCR fragments
attp
IR
aph Ⅱ
ligation
IR
p15A
pTP-aph
cat
3998 bp
KpnI
aph Ⅱ
ligation
IR
p15A-aph-cat
aph Ⅱ
1978 bp
p15A
KpnI
IR
NdeI
cat
NdeI
Tpase
NdeI
PCR fragments
KpnI
Nde Ⅰ digestion with ends blunted
Mx8
attp
p15A
ligation
pMx8-aph
4970 bp
PCR fragments
cat
aph Ⅱ
attp