Additional file 14
A
Glucose alone
0h
after 2h
6000
4000
3000
2000
Intensity (a.u.)
5000
6000
5000
4000
3000
2000
1000
1000
0
0
160 140 120 100 80
60
40
chemical shift(ppm)
B
20
160 140 120 100 80
0
0h
Q0
60
40
chemical shift (ppm)
20
0
after 2h
16000
2800
12000
2000
8000
1200
4000
400
0
160 140 120 100 80
60
40
20
0
160 140 120 100 80
0
chemical shift (ppm)
C
0h
Q56
60
40
20
0
chemical shift (ppm)
after 2h
5000
19000
4000
15000
3000
11000
2000
7000
1000
3000
0
160 140 120 100 80
60
40
20
0
0
160 140 120 100 80
chemical shift (ppm)
60
40
20
0
chemical shift (ppm)
E
Normalized peak intensity 124
Normalized peak intensity 60
D
1.0
0.8
0.6
0.4
0.2
0.0
0
1
2
3
time (h)
4
5
1.0
0.8
0.6
0.4
0.2
0.0
0
1
2
3
time(h)
4
5
F
Et
Et
Et
Et
BY4717 Q0-YFP 2h
see G
BY4741 Q56-YFP 2h
see G
G
240
230
220
210
200
190
180
170
160
150
140
130
120
110
100
90
chemical shift (ppm)
G
G G
80
70
G
60
Pyr
50
40
30
20
10
0
-10
Carbonate
G
BY4717 Q0-YFP 2h
BY4741 Q56-YFP 2h
Carbonate
Pyr
Pyr
210 208 206 204 202 200 198 196 194 192 190 188 186 184 182 180 178 176 174 172 170 168 166 164 162 160 158 156 154 152 150 148
chemical shift (ppm)
-20