Supplementary information

Development 143: doi:10.1242/dev.126227: Supplementary information
Figure S1. Minimal effect of Smad4 deletion on preosteoblast proliferation and osteoblast
activity. (A) Quantification of BrdU-positive cells (%) at chondro-osseous junction on tibial sections
at P33. (B,C) Dynamic histomorphometry parameters at P60. WT: Smad4f/f; Smad4: Dmp1-Cre;
Figure S2. qRT-PCR analyses with RNA from calvaria of mice at P33. Data shown as fold
change over 18S. WT: Bmpr1af/f; MT: Dmp1-Cre; Bmpr1af/f. *: p<0.05, n=3.
Development • Supplementary information
Smad4f/f. *: p<0.05, n=3.
Development 143: doi:10.1242/dev.126227: Supplementary information
Development • Supplementary information
Table S1. qRT-PCR primer sequences.