Development • Supplementary information
Development 143: doi:10.1242/dev.126383: Supplementary information
Development • Supplementary information
Development 143: doi:10.1242/dev.126383: Supplementary information
Development • Supplementary information
Development 143: doi:10.1242/dev.126383: Supplementary information
Development • Supplementary information
Development 143: doi:10.1242/dev.126383: Supplementary information
Development 143: doi:10.1242/dev.126383: Supplementary information
Table S1. Mef2c+/-; Tdgf1+/- compound heterozygotes do not display an overt phenotype.
Mef2c+/- mice were crossed to Tdgf1+/- mice to generate Mef2c+/-; Tdgf1+/- compound
heterozygous mice. Mice were counted at weaning (P21), and the number of observed and
expected offspring of each indicated genotype was counted. There was no significant deviation
of the observed number of offspring from the expected number. χ2 test, , p=0.8735.
genotype
observed
expected
wild type
20
18.25
Tdgf1+/-
17
18.25
Mef2c+/-
16
18.25
Tdgf1+/-; Mef2c+/-
20
18.25
Development • Supplementary information
Tdgf1+/- x Mef2c+/- intercross Development 143: doi:10.1242/dev.126383: Supplementary information
Table S2. qPCR primers used in this study. Note that the Mef2c primers detect the deleted
Gene
Forward primer
Reverse Primer
gapdh
5ʹ′-catgaccacagtccatgc-3ʹ′
5ʹ′-agtgagcttcccgttcag-3ʹ′
Mef2c
5ʹ′-ggccatggtacaccgagtacaacga-3ʹ′
5ʹ′-ggggatccctgtgttacctgcacttgg-3ʹ′
Tdgf1
5ʹ′-cctccttccttctatggacgca-3ʹ′
5ʹ′-aggtctgaggaagacagtggag-3ʹ′
Development • Supplementary information
(floxed) exon.