Table S3. Primers used in this study
Fragmenta
sequence (5'->3')
locus tagb
CAGGAAATTTGATTACTACATACCCAGTA
NMC0444
GGAGGCTAAAGCTGCGGTTTGCATGGCAGCTGC
NMC0446
CAGGAAATTTGATTACTACATACCCAGTA
NMC0444
GGATCCTTATTGGAATTTAAGCTCCAATTTAGTA
NMC0446
CCATTGACATCAGCGGTGTA
NMC0444
a1
amplicon sizec
1098
a2
2651
b
2390
AGCCAGCAGATACCGGTTGTCCCGTTTGTCTCA
NMC0444
CCATTGACATCAGCGGTGTA
NMC0444
GGATCCTTATTGGAATTTAAGCTCCAATTTAGTA
NMC0446
ACTGCAGAAAAGAATCTGGA
NMC0444
CCACCCAGTGTGGTTTCAA
NMC0444
GCCAAGGCCGATGTCAATGC
NMA0688
TGCCGCCTGCCTGAAGCGT
NMA0688
GAAATTGGCAAGCCCACCTA
NMB0497
c
5342
d
2280
e
1596
f
2415
AGCCAGCAGATACCGGTTGTCCCGTTTGTCTCA
NMB0497
remarksd
GAAATTGGCAAGCCCACCTA
NMA0688
GCAGTCATTTCGTTATCAA
NMA0690
GAGGATCCGAATTGCGCCACCGTGCTTA
NMC0443
BamHI
NMC0443 / NMC0444
PstI
AAAGGCGCCATTTACAAAGGATTTTACCG
NMC0447
KasI
AAAAAACACAACGTGGGGACACATTAAAAACAGAAT
NMC0448
DraIII
AAACTGCAGTGATTTTCTACTTAAATCAAGCTACCTTAA
NMC0458
PstI
AAACATATGTTATTTTATCTTCTGAAAGAAAATTATTTTTTT
NMC0459
NdeI
TGGACATTAGATTTGCAGAATCC
NMC0446
AAAGCCGGCTATGCAGAAATGATTTTATT
NMC0448
AATGATTCATCCGTATATCT
NMC0448
GGATCCCTATTTTCCTGTAAAAATATTTAATTG
NMC0450
CTCTCCTCCTAGAGGAACAT
NMC0450
g
5210
h1
CTCTGCAGGTTTGACGGTCTGAGGATAAGGAAAGA
h2
i
j
2222
k
2853
l
2817
TTGTCCACGGCTCTCTAGGATTCCTCATA
NMC0452
GTACACCTGCTTCGCTAAG
NMC0452
AGAGTCAAATAACTGCTCGGTCATTTTAC
NMC0456
TATGATTTACAATACTCTGCTGAT
NMC0456
TAATAGCAAGAGTCTGAAGCAAA
NMC0459
m
1862
n
2073
GAAATTGGCAAGCCCACCTA
NMB1779
GGATCCTTATTGGAATTTAAGCTCCAATTTAGTA
NMB1779
p
2774
GCTGCCTGTTATCCTGCAAACAGA
Upstream NMB1780
3303
q
CAAAGGATCCTAAGCATTTAATACCGTGGTAGCTGGGCGA
NMB1779
GCCCTCGCGTTTGGCATTTTCAGCAA
NMB0493
CCGAGTACACGGTGTTTCATCTTCACT
NMB0496
CATATGGCTGCTCCTAAAGAAACAATAAAC
NMC0446
AGCGTCGACTTCAGACGGCCACGTTGTGTC
kan cassette
CCATTGACATCAGCGGTGTA
NMO_0398
AGCGTCGACTTCAGACGGCCACGTTGTGTC
kan cassette
CCATTGACATCAGCGGTGTA
NMO_0398
AGAGTCAAATAACTGCTCGGTCATTTTAC
NMO_0398
GAAATTGGCAAGCCCACCTA
NME_0860
GGATCCTTATTGGAATTTAAGCTCCAATTTAGTA
NME_0860
CCACAGCCCCACGTTTGCGGTT
NMB1771
CAGGATCCATTTTCACCTAAACTATGCACGCTAGA
NMB1768
r
1875
r1
r2
r3
2623
r4
2722
s
t
1710
GAAATTGGCAAGCCCACCTA
CAAAGACTACCACCAACA
NMB1779 / NMB0497
NMB0511
CGCGCGCATATGAGTAACTTTGAAAAAAAATATA
NMC0445
v
GCGCGCGACGTCTTATTTAAATTCATCACGTTTCA
a
NdeI
489
NMC0445
Letters correspond to the PCR fragments indicated in Figures S4 and S6 in Additional file 1and Table S2 in Additional file 2.
Locus tags are from the genome sequences of FAM18, Z2491, MC58, α14 and α153.
c
Amplicon size given is based on the available genome sequences of the reference strains.
d
Restriction enzymes used for cloning. The restriction sites are underlined in the primer sequences.
b
AatII