Table S2. Meningococcal isolates used in this study and results of PCR and antisera reactions
Isolate
a
B16B6#
FAM18#
59
69
102
115
142
156
175
177
187
192
197
222
295
307
314
326
328
348
354
382
385
399
413
422
423
427
433
435
442
448
450
455
461
470
475
479
851100
851452
900106
900234
900707
901140
902208
902286
990056
990602
b
Isolation
year
Geographic
origin
Source
1960
1983
1960
1960
1960
1960
1961
1961
1961
1961
1961
1962
1962
1962
1963
1963
1963
1963
1963
1963
1963
1963
1963
1963
1963
1964
1964
1964
1964
1964
1964
1964
1964
1964
1964
1964
1964
1964
1985
1985
1990
1990
1990
1990
1990
1990
1999
1999
United States
United States
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
Invasive
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
Blood
CSF
CSF
CSF
CSF
CSF
CSF
CSF
Blood
CSF
CSF
CSF
CSF
CSF
CSF
Blood
Blood
CSF
CSF
CSF
Blood
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
c
Serogroup
ST
Clonal
Complex
PCR
B
C
B
B
B
B
C
B
B
B
B
C
C
B
C
B
C
C
B
C
C
C
C
B
C
C
C
B
C
B
C
C
C
C
B
C
C
C
B
B
B
B
C
C
C
C
C
C
11
11
11
11
11
11
11
6010
6015
11
11
6824
11
6881
6831
11
6833
11
11
11
11
11
11
11
11
11
4706
11
11
11
11
11
11
4706
11
11
11
11
11
11
11
11
11
11
11
2510
51
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
a1,b,c,d
a1,b,c,d
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
g
a1
a1,b,c,d
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1,b,c,d
a1
a1
a1
a1,b,c,d
a1
Anti
serad
nd
nd
nd
a, b
nd
nd
nd
nd
nd
nd
nd
nd
a
nd
nd
nd
nd
nd
b
nd
b
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
a, b
nd
nd
a, b
nd
nd
nd
nd
990738
990797
990947
991044
991174
991379
991930
991932
992062
992076
2000151
2000171
2000321
2000345
2000449
2000739
2000779
2000780
2000811
2000844
2000907
2000948
2000974
2001044
2001188
2001212
2001215
2001271
2001329
2001330
2001428
2001477
2001540
2001622
2001623
2001633
2001718
2001730
2001848
2001861
2001953
2002038
1999
1999
1999
1999
1999
1999
1999
1999
1999
1999
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
Eye
Blood
CSF
CSF
Blood
Blood
CSF
Blood
Blood
CSF
CSF
Blood
CSF
CSF
Blood
Blood
CSF
CSF
CSF
Blood
CSF
CSF
CSF
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
W135
W135
W135
W135
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
11
5450
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
2704
1055
11
11
11
11
11
11
11
11
11
11
11
51
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
a1
c1
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1,b,c,d
a1
a1
a1
nd
nd
a
nd
nd
nd
nd
nd
nd
nd
nd
a, b
nd
nd
nd
a
nd
nd
nd
nd
a
nd
nd
a
nd
nd
nd
nd
nd
nd
a
nd
nd
nd
nd
b
nd
nd
a
nd
nd
nd
2002173
2002188
2010131
2010178
2010250
2010291
2010351
2010394
2010443
2010445
2000
2000
2001
2001
2001
2001
2001
2001
2001
2001
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
CSF
CSF
CSF
CSF
Blood
Blood
Blood
CSF
Blood
Blood
C
W135
C
C
C
C
W135
C
C
C
3456
11
11
11
11
11
247
11
11
11
11
11
11
11
11
11
11
11
11
11
a1,b,c,d
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
nd
nd
nd
nd
nd
nd
nd
nd
a
2010478
2010513
2010563
2010664
2010699
2010760
2010816
2010818
2010878
2010905
2010938
2010939
2010962
2010980
2011000
2011043
2011061
2011150
2011186
2011212
2011215
2011255
2011328
2011333
2011334
2011335
2011358
2011371
2011471
2011528
2011545
2011576
2011603
2011674
2011692
2011709
2011728
2011748
2011780
2011784
2011785
2011791
2011792
2011793
2011801
2011805
2011814
2011815
2011823
2011831
2011832
2011852
2011872
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
Blood
CSF
CSF
CSF
CSF
CSF
CSF
Blood
CSF
Blood
Blood
CSF
CSF
Blood
CSF
CSF
Blood
CSF
Blood
CSF
CSF
Blood
Blood
Blood
CSF
CSF
CSF
CSF
CSF
CSF
Blood
Blood
CSF
CSF
CSF
Blood
CSF
CSF
CSF
Blood
Blood
Blood
Blood
CSF
Blood
CSF
Blood
Blood
Blood
CSF
CSF
CSF
Blood
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
B
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
2704
11
2704
11
11
11
11
4283
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
247
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1,b,c,d
a1
a1
a1,b,c,d
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
nd
nd
nd
nd
nd
a
nd
nd
nd
nd
nd
nd
nd
nd
b
nd
nd
nd
a
nd
nd
b
nd
a, b
nd
nd
nd
nd
nd
nd
nd
a
nd
nd
nd
nd
nd
nd
nd
nd
a
nd
nd
nd
nd
nd
a, b
nd
nd
nd
nd
nd
2011929
2011940
2011975
2011978
2011980
2012005
2012024
2012062
2012099
2012140
2012199
2012210
2012278
2012280
2012300
2012322
2012341
2012376
2012402
2012427
2012431
2012448
2012526
2012532
2012588
2012598
2012602
2012620
2012622
2012655
2012671
2012673
2012717
2020004
2020017
2020041
2020047
2020130
2020145
2020148
2020212
2020218
2020227
2020236
2020258
2020279
2020322
2020328
2020349
2020370
2020397
2020435
2020448
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
CSF
CSF
Blood
CSF
CSF
CSF
Blood
CSF
Blood
Blood
Blood
Blood
Blood
CSF
Blood
CSF
Blood
Blood
Blood
Blood
CSF
Blood
CSF
Blood
Blood
CSF
CSF
CSF
Blood
CSF
CSF
CSF
CSF
CSF
CSF
Blood
CSF
CSF
CSF
Blood
CSF
Blood
CSF
CSF
CSF
Blood
Blood
CSF
Blood
Blood
CSF
CSF
CSF
C
W135
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
W135
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
11
11
247
247
11
11
11
11
11
11
11
11
11
11
11
11
11
11
2706
11
11
11
11
11
11
11
11
11
11
11
11
2709
11
11
11
11
11
11
11
11
11
11
1254
11
11
11
11
11
11
11
3035
3298
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
c1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
a
nd
nd
nd
nd
b
nd
nd
nd
nd
a
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
a, b
nd
nd
nd
nd
nd
nd
nd
nd
a
nd
nd
b
nd
nd
nd
nd
a, b
nd
nd
nd
2020503
2020515
2020530
2020533
2020540
2020561
2020570
2020602
2020629
2020637
2020656
2020680
2020690
2020693
2020712
2020730
2020748
2020844
2020875
2020878
2020910
2020955
2020968
2020982
2021027
2021181
2021264
2021267
2021306
2021322
2021335
2021352
2021468
2021481
2021708
2021728
2021740
2021775
2021819
2021907
2021940
2022024
2022505
2030100
2030399
2030488
2030689
2031022
2031338
2031356
2031499
2031503
2031588
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2002
2003
2003
2003
2003
2003
2003
2003
2003
2003
2003
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
CSF
CSF
Blood
Blood
CSF
CSF
Blood
Blood
Blood
Blood
CSF
Blood
Blood
CSF
CSF
CSF
CSF
Blood
CSF
Blood
Blood
Blood
Blood
CSF
CSF
Blood
Blood
CSF
Blood
Blood
CSF
Blood
CSF
Blood
Blood
CSF
Blood
CSF
CSF
CSF
CSF
CSF
CSF
CSF
Blood
CSF
Blood
CSF
CSF
CSF
Blood
Blood
Blood
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
B
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
11
11
11
11
11
11
11
11
11
3726
11
11
11
11
11
11
11
11
11
11
11
11
11
3732
11
11
11
11
11
11
11
11
11
11
2704
11
11
11
3456
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1
a1
a1
a1,b,c,d
nd
nd
nd
nd
nd
nd
a
nd
nd
nd
nd
nd
b
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
a, b
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
a, b
nd
nd
nd
nd
a, b
nd
nd
nd
nd
nd
nd
nd
a, b
2032645
2032737
2040215
2040237
2041114
2041350
2050123
2051166
2051624
2060153
2061255
2070078
2071345
2071415
2071442
2071467
2071886
2080098
2080150
2080184
α122
α223
α306
α324
α737
α738
2996#
149
189
227
245
424
1679
1685
1692
1707
1717
1726
1744
1767
1768
1789
1796
1828
1838
1914
1926
1949
800001
800062
800083
800094
800236
2003
2003
2004
2004
2004
2004
2005
2005
2005
2006
2006
2007
2007
2007
2007
2007
2007
2008
2008
2008
1999/00
1999/00
1999/00
1999/00
1999/00
1999/00
1975
1961
1962
1962
1962
1964
1970
1970
1970
1970
1970
1970
1970
1970
1970
1970
1970
1970
1970
1970
1970
1970
1980
1980
1980
1980
1980
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
Germany
Germany
Germany
Germany
Germany
Germany
United kingdom
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
Blood
Blood
Blood
CSF
Blood
Blood
Blood
CSF
CSF
Blood
Blood
CSF
CSF
CSF
CSF
CSF
Blood
CSF
Blood
CSF
NSF
NSF
NSF
NSF
NSF
NSF
invasive
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
Blood
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
CSF
Blood
CSF
CSF
CSF
C
C
C
C
C
B
C
W135
B
C
C
C
C
W135
C
W135
C
C
C
C
W135
C
W135
C
C
C
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
11
11
11
11
1026
5412
11
11
11
11
11
3298
11
11
11
11
11
211
6872
11
11
11
11
11
11
11
540
6008
8
8
6884
8
487
8
8
8
8
8
4860
2174
8
8
8
8
8
8
8
2174
6853
8
8
8
9
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1,b,c,d
a1
a1
a1
a1,a2,b
a1,a2,b
a1,a2,b
a1,a2,b
a1,a2,b
a1,a2,b
a1,a2,b
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1,b,d
a1,b,d
a1,b,d
a1
a1
nd
nd
a, b
nd
nd
nd
nd
nd
nd
nd
nd
a
nd
nd
nd
nd
nd
nd
b
a, b
a
b
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
800254
800297
800426
800624
800702
801356
850223
850272
850387
850519
851347
902388
982084
991210
2000221
2000235
2000640
2000863
2000966
2001031
2001173
2001346
2001359
2001509
2001649
2001821
2002111
2010115
2010221
2010383
2010403
2010511
2010745
2010937
2011060
2011437
2011578
2011726
2011899
2012063
2012403
2012552
2012637
2012653
2012711
2020151
2020745
2020907
2020933
2020945
2020994
2021497
2021581
1980
1980
1980
1980
1980
1980
1985
1985
1985
1985
1985
1990
1998
1999
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2001
2002
2002
2002
2002
2002
2002
2002
2002
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
CSF
CSF
CSF
CSF
CSF
Blood
CSF
Blood
CSF
CSF
CSF
CSF
CSF
Blood
Blood
CSF
CSF
CSF
CSF
Blood
CSF
CSF
CSF
CSF
CSF
CSF
Blood
CSF
CSF
CSF
CSF
CSF
CSF
Blood
CSF
Blood
Blood
CSF
Blood
Blood
Throat
CSF
Blood
CSF
Blood
CSF
CSF
Blood
CSF
CSF
CSF
CSF
Blood
B
B
B
B
B
B
B
B
B
B
B
B
C
C
C
C
C
C
C
C
C
C
B
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
6856
2174
8
8
4611
8
9
335
7102
8
9
153
66
2680
66
2680
66
2680
66
2680
4428
2680
4429
2699
66
2680
2680
2699
2680
2680
2699
2699
8
2680
2699
2680
66
1372
2680
2680
2680
2680
2680
2680
8
3486
2680
1372
2680
2680
2680
2680
3522
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1
a1,b,d
a1,b,d
a1
a1
a1
a1
a1
a1
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
2021671
2040210
2040355
2040362
2041497
2060591
2061599
2071065
2071129
2071211
2071237
2080039
2080070
2002
2004
2004
2004
2004
2006
2006
2007
2007
2007
2007
2008
2008
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
2071282
2092035
2007
2009
The Netherlands
The Netherlands
2090919
2090516
2090354
2090110
2009
2009
2009
2009
The Netherlands
The Netherlands
The Netherlands
The Netherlands
2090092
2082287
2082151
2081656
2009
2008
2008
2008
The Netherlands
The Netherlands
The Netherlands
The Netherlands
2081056
2008
The Netherlands
2080724
2008
The Netherlands
2080676
2080654
2080564
2080448
2080151
2071909
2071749
2071499
2008
2008
2008
2008
2008
2007
2007
2007
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
2071291
2071066
2070973
2070947
2070929
2007
2007
2007
2007
2007
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
2070861
2007
The Netherlands
2070755
2070582
2070472
2070206
2007
2007
2007
2007
The Netherlands
The Netherlands
The Netherlands
The Netherlands
Blood
CSF
Blood
CSF
CSF
Blood
CSF
CSF
Blood
CSF
Blood
Blood
blood /
CSF
CSF
blood /
CSF
blood
blood
CSF
blood /
CSF
blood
CSF
CSF
blood /
CSF
blood /
CSF
blood /
CSF
CSF
CSF
joint
blood
blood
blood
blood
blood /
CSF
blood
blood
blood
CSF
blood /
CSF
blood /
CSF
blood
CSF
throat
blood /
CSF
C
C
B
C
C
C
C
C
C
C
C
C
B
1372
8
153
8
1372
66
8
6859
1372
6861
1372
1372
32
8
8
8
8
8
8
8
8
8
8
8
8
32
a1,b,d
a1,b,d
a1,b,d
a1
a1
a1
a1
a1
a1
a1
a1
a1
2
f ,p,e2
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
B
B
259
34
32
32
f,p,e2
f,p,e,v
nd
nd
B
B
B
B
34
32
3720
33
32
32
32
32
f,p,e,v
f,e,v,q
f,p,e,v
f,p,e,v
nd
nd
nd
nd
B
B
B
B
34
33
3720
32
32
32
32
32
f,p,e,v
f,e,v,q
f,p,e,v
f,e,v,q
nd
nd
nd
nd
B
34
32
f,p,e,v
nd
B
7884
32
f,e,v,q
nd
B
B
B
B
B
B
B
B
32
34
34
34
32
3720
34
5138
32
32
32
32
32
32
32
32
f,p,e,v
f,p,e,v
f,p,e,v
f,p,e,v
f,p,e
f,p,e,v
f,p,e,v
f,p,e,q
nd
nd
nd
nd
nd
nd
nd
nd
B
B
B
B
B
3720
34
34
6299
32
32
32
32
32
32
f,p,e,v
f,p,e
f,p,e,v
f,e,v2,q
f,e,v,q
nd
nd
nd
nd
nd
B
32
32
f,e,v,q
nd
B
B
B
B
4949
33
6292
3720
32
32
32
32
f,p,e,v
f,e,v,q
f,p,e,q
f,p,e,v
nd
nd
nd
nd
2070118
2007
The Netherlands
2070093
2070077
2061546
2061481
2061322
2061285
2061227
2061192
2061147
2061119
2007
2007
2006
2006
2006
2006
2006
2006
2006
2006
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
2060978
2060939
2060640
2060602
2060489
2081107
2006
2006
2006
2006
2006
2008
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
The Netherlands
blood /
CSF
CSF
blood
blood
blood
blood
CSF
CSF
blood
CSF
blood /
CSF
blood
blood
blood
CSF
blood
blood
B
34
32
f,p,e,v
nd
B
B
B
B
B
B
B
B
B
B
3720
34
34
32
32
3720
5955
34
3720
33
32
32
32
32
32
32
32
32
32
32
f,p,e,v
f,p,e,
f,p,e,v
f,p,e
f,e,v,q
f,p,e,v
f,e,q
f,p,e,v
f,p,e,v
f,p,e,v
nd
nd
nd
nd
nd
nd
nd
nd
nd
nd
B
B
B
B
B
B
34
34
3720
33
33
34
32
32
32
32
32
32
f,p,e,v
f,p,e,v
f,p,e,v
f,p,e
f,p,e
f,q,r,s,
d2,b
f,q
f,q,r,s,t
r4
r3
nd
nd
nd
nd
nd
nd
2061646
2006
The Netherlands
CSF
B
32
32
nd
2061468
2006
The Netherlands
blood
B
34
32
nd
α153#
1999/00
Germany
NSF
29E
60
60
nd
α14#
1999/00
Germany
NSF
cnI
53
53
nd
a
#, Neisseria reference strains.
b
Invasive, disease isolate without specified origin; CSF, cerebrospinal fluid; NSF, carrier isolates from
nasopharynx.
c
DNA fragments amplified by PCR as specified in Figures S4 and S6 in Additional file 1 and Table S3 in
Additional file 3. Superscripts 1 and 2 indicate that the amplicons were smaller and larger, respectively, than
expected. Note that all cc11 and cc8 isolates were initially tested for amplification of fragment a1, while only
some of them were subsequently tested for other fragments. Results for fragments j-n are not shown.
Fragments e and v indicate positive tests for the presence of the pilE locus and the same hypervariable region
of pilE as in MC58, respectively. Fragments q, r and s were tested in cc32 isolates negative for fragment p.
The last two strains listed were not extensively evaluated but used for specific purposes only; only fragments
obtained for those assays are indicated.
d
Positive reactions in Western blots with antiserum directed against NalP (a) and a mixture of monoclonal
antibodies recognizing OpaA, OpaB, OpaD and OpaJ (b) are indicated. nd, no reactions determined.