Supplementary information

Development 143: doi:10.1242/dev.128900: Supplementary information
Development • Supplementary information
Fig. S1. Comparison of ctsba exon reads in wild-type and split top mutant
embryos using the Integrated Genomic Viewer. In split top mutant embryos
ctsba transcript reads are greatly reduced relative to wild-type. In split top mutant
embryos, exons 1, 6, 7 are not covered by any transcripts while exons 2-5, 8-10
have minimal coverage (≤4 transcripts per exon). Only a subset of the wild-type
ctsba reads are shown.
Development 143: doi:10.1242/dev.128900: Supplementary information
Development • Supplementary information
Movie 1. Time-lapse imaging of wild-type zebrafish embryo. Wild-type embryo
development from blastula to somitogenesis stages.
Development 143: doi:10.1242/dev.128900: Supplementary information
Development • Supplementary information
Movie 2. Time-lapse imaging of split top mutant embryo phenotypes. split top
mutant embryo highlighting the developmental delay, separation of the EVL and the
deep cells, and the lysis phenotype.
Development 143: doi:10.1242/dev.128900: Supplementary information
Development • Supplementary information
Movie 3. Time-lapse imaging of split top mutant split-yolk phenotype. split top
mutant embryo highlighting the development of the split-yolk phenotype.
Development 143: doi:10.1242/dev.128900: Supplementary information
Development • Supplementary information
Movie 4. Time-lapse imaging of split top mutant early lysis phenotype. split top
mutant embryo highlighting the development of the early lysis phenotype.