Huck Hui NG, Ph.D.
Executive Director
[email protected]
RESEARCH FOCUS
In general, transcriptional activation results from the tethering of sequence specific DNA binding
transcription factors to their cognate sites. This leads to the assembly of transcription initiation
complex at promoters and subsequent transcription elongation. The mammalian genome encodes
for over 2000 transcription regulators, and many of them play critical roles in orchestration of gene
expression which is fundamental to cellular phenotypes and identity of the organism. Our long term
goal is to dissect the functional role of transcription regulators using two complementary
technologies (1. chromatin immunoprecipitation to study the location of transcription factors in
living cells, and 2. RNAi depletion to examine the response of cells in the absence of the factors).
This experimental strategy will allow us to probe the functional importance of the transcription
factors of interest. We have setup a system for large scale RNAi to screen for transcription factors
(or other proteins) involved in the regulation of cell-type specific promoter. Complemented with
ChIP, we are able to dissect the functional importance of binding of transcription factors to key
regulatory elements.
Our focus is on stem cell biology. We are addressing two questions:
1) What makes a stem cell a stem cell?
2) How to make a non-stem cell a stem cell?
EDUCATION
1996-1999
1992-1996
PhD. University of Edinburgh Advisor: Adrian Bird
B.Sc (Honours 1st class) National University of Singapore Advisor: Eng Chong Pua
and Ben Li
PROFESSIONAL APPOINTMENTS
2012
201120072008200920092003-2007
2003-2009
2000-2003
Executive Director, Genome Institute of Singapore
Deputy Director (Research Programmes)
Senior Group Leader, Genome Institute of Singapore
Adjunct Associate Professor (School of Biological Sciences, Nanyang Technological
University)
Adjunct Associate Professor (Department of Biochemistry, National University of
Singapore)
Adjunct Associate Professor (Department of Biological Sciences, National University
of Singapore)
Group Leader, Genome Institute of Singapore
Assistant Professor (Department of Biological Sciences, National University of
Singapore)
Postdoctoral fellow, Harvard Medical School Advisor: Kevin Struhl
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HONORS AND AWARDS
2011
2010
2010
2009
2008
2007
2005
2004
2003
2000
1996
1996
President’s Science Award
Singapore Youth Award (Medal of commendation) for Science and Technology
Chen New Investigator Award (Chens’ Foundation and Human Genome Organisation)
The Young Outstanding Persons Award of Singapore (Scientific and/or technological
development)
Outstanding Scientist Award (Faculty of Science, National University of Singapore)
National Science Award
Singapore Youth Award (Individual) for Science and Technology
Singapore Young Scientist Award
BMRC Young Investigator Award
Damon Runyon-Walter Winchell Postdoctoral fellowship
Darwin Trust of Edinburgh Scholarship
Lijen Industrial Development Medal (National University of Singapore)
EXTERNAL GRANT
2006 to 2009
Human Stem Cell Epigenome Project by SSCC, BMRC (-)
COMPLETE PUBLICATION LIST
1. Ng HH "2013 runme shaw memorial lecture: clinical applications of stem cells in modern
medicine-21st century and beyond." Ann Acad Med Singapore 2014 Jan ; 43(1) : 68-71
2. Isasi R, Andrews PW, Baltz JM, Bredenoord AL, Burton P, Chiu IM, Hull SC, Jung JW, Kurtz A,
Lomax G, Ludwig T, McDonald M, Morris C, Ng HH, Rooke H, Sharma A, Stacey GN, Williams C, Zeng
F, Knoppers BM "Identifiability and privacy in pluripotent stem cell research." Cell Stem Cell 2014
Apr 3 ; 14(4) : 427-30
3. Fang F, Xu Y, Chew KK, Chen X, Ng HH, Matsudaira P "Coactivators p300 and CBP maintain the
identity of mouse embryonic stem cells by mediating long-range chromatin structure." Stem Cells
2014 Mar 19
4. Lu X, Sachs F, Ramsay L, Jacques PÉ, Göke J, Bourque G, Ng HH "The retrovirus HERVH is a long
noncoding RNA required for human embryonic stem cell identity." Nat Struct Mol Biol 2014 Apr ;
21(4) : 423-5 Epub 2014 Mar 30
5. Isasi R, Andrews PW, Baltz JM, Bredenoord AL, Burton P, Chiu IM, Hull SC, Jung JW, Kurtz A,
Lomax G, Ludwig T, McDonald M, Morris C, Ng HH, Rooke H, Sharma A, Stacey GN, Williams C, Zeng
F, Knoppers BM "Identifiability and privacy in pluripotent stem cell research." Cell Stem Cell 2014
Apr 3 ; 14(4) : 427-30
6. Lu X, Ng HH, Bubulya PA "The role of SON in splicing, development, and disease." Wiley
Interdiscip Rev RNA 2014 Apr 30
7. Chia NY, Deng N, Das K, Huang D, Hu L, Zhu Y, Lim KH, Lee MH, Wu J, Sam XX, Tan GS, Wan
WK, Yu W, Gan A, Tan AL, Tay ST, Soo KC, Wong WK, Dominguez LT, Ng HH, Rozen S, Goh LK, Teh
BT, Tan P "Regulatory crosstalk between lineage-survival oncogenes KLF5, GATA4 and GATA6
cooperatively promotes gastric cancer development." Gut 2014 Jul 22
8. Tam WL, Ng HH "Sox2: masterminding the root of cancer." Cancer Cell 2014 Jul 14 ; 26(1) : 3-5
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9. Muratani M, Deng N, Ooi WF, Lin SJ, Xing M, Xu C, Qamra A, Tay ST, Malik S, Wu J, Lee MH,
Zhang S, Tan LL, Chua H, Wong WK, Ong HS, Ooi LL, Chow PK, Chan WH, Soo KC, Goh LK, Rozen S,
Teh BT, Yu Q, Ng HH, Tan P "Nanoscale chromatin profiling of gastric adenocarcinoma reveals
cancer-associated cryptic promoters and somatically acquired regulatory elements." Nat Commun
2014 ; 5 : 4361
10. Yeo JC, Jiang J, Tan ZY, Yim GR, Ng JH, Göke J, Kraus P, Liang H, Gonzales KA, Chong HC, Tan
CP, Lim YS, Tan NS, Lufkin T, Ng HH "Klf2 is an essential factor that sustains ground state
pluripotency." Cell Stem Cell 2014 Jun 5 ; 14(6) : 864-72
11. Gonzales KA, Ng HH "Driving pluripotency and reprogramming: nuclear receptors at the helm."
Semin Cell Dev Biol 2013 Dec ; 24(10-12) : 670-8 Epub 2013 Aug 2
12. Chan YS, Göke J, Ng JH, Lu X, Gonzales KA, Tan CP, Tng WQ, Hong ZZ, Lim YS, Ng HH
"Induction of a human pluripotent state with distinct regulatory circuitry that resembles
preimplantation epiblast." Cell Stem Cell 2013 Dec 5 ; 13(6) : 663-75
13. Lu X, Göke J, Sachs F, Jacques PÉ, Liang H, Feng B, Bourque G, Bubulya PA, Ng HH "SON
connects the splicing-regulatory network with pluripotency in human embryonic stem cells." Nat
Cell Biol 2013 Oct ; 15(10) : 1141-52 Epub 2013 Sep 8
14. Ng HH, Tam PP "The mystique of cellular reprogramming." Curr Opin Genet Dev 2013 Oct ;
23(5) : 501-3 Epub 2013 Sep 1
15. Gonzales KA, Ng HH "Driving pluripotency and reprogramming: Nuclear receptors at the helm."
Semin Cell Dev Biol 2013 Aug 2
16. Do DV, Ueda J, Messerschmidt DM, Lorthongpanich C, Zhou Y, Feng B, Guo G, Lin PJ, Hossain
MZ, Zhang W, Moh A, Wu Q, Robson P, Ng HH, Poellinger L, Knowles BB, Solter D, Fu XY "A genetic
and developmental pathway from STAT3 to the OCT4-NANOG circuit is essential for maintenance of
ICM lineages in vivo." Genes Dev 2013 Jun 15 ; 27(12) : 1378-90
17. Kumar V, Muratani M, Rayan NA, Kraus P, Lufkin T, Ng HH, Prabhakar S "Uniform, optimal
signal processing of mapped deep-sequencing data." Nat Biotechnol 2013 Jun 16 Epub 2013 Jun 16
18. Göke J, Chan YS, Yan J, Vingron M, Ng HH "Genome-wide Kinase-Chromatin Interactions Reveal
the Regulatory Network of ERK Signaling in Human Embryonic Stem Cells." Mol Cell 2013 May 29
19. Karwacki-Neisius V, Göke J, Osorno R, Halbritter F, Ng JH, Weiße AY, Wong FC, Gagliardi A,
Mullin NP, Festuccia N, Colby D, Tomlinson SR, Ng HH, Chambers I "Reduced Oct4 expression
directs a robust pluripotent state with distinct signaling activity and increased enhancer occupancy
by Oct4 and Nanog." Cell Stem Cell 2013 May 2 ; 12(5) : 531-45
20. Ng JH, Kumar V, Muratani M, Kraus P, Yeo JC, Yaw LP, Xue K, Lufkin T, Prabhakar S, Ng HH "In
vivo epigenomic profiling of germ cells reveals germ cell molecular signatures." Dev Cell 2013 Feb
11 ; 24(3) : 324-33 Epub 2013 Jan 24
21. Yeo JC, Ng HH "The transcriptional regulation of pluripotency." Cell Res 2013 Jan ; 23(1) :
20-32 Epub 0201 Feb 12
22. Chan YS, Göke J, Lu X, Venkatesan N, Feng B, Su IH, Ng HH "A PRC2 Dependent Repressive
role of PRDM14 in Human Embryonic Stem Cells and iPSC Reprogramming." Stem Cells 2012 Dec
28
23. Zhao T, Goh KJ, Ng HH, Vardy LA "A role for polyamine regulators in ESC self-renewal." Cell
Cycle 2012 Dec 15 ; 11(24) : 4517-23 Epub 2012 Nov 19
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24. Percharde M, Lavial F, Ng JH, Kumar V, Tomaz RA, Martin N, Yeo JC, Gil J, Prabhakar S, Ng HH,
Parker MG, Azuara V "Ncoa3 functions as an essential Esrrb coactivator to sustain embryonic stem
cell self-renewal and reprogramming." Genes Dev 2012 Oct 15 ; 26(20) : 2286-98 Epub 2012 Sep
26
25. Orlov Y, Xu H, Afonnikov D, Lim B, Heng JC, Yuan P, Chen M, Yan J, Clarke N, Orlova N, Huss M,
Gunbin K, Podkolodnyy N, Ng HH "Computer and Statistical Analysis of Transcription Factor Binding
and Chromatin Modifications by ChIP-seq data in Embryonic Stem Cell." J Integr Bioinform 2012 ;
9(2) : 211 Epub 2012 Sep 18
26. Chan YS, Yang L, Ng HH "Transcriptional regulatory networks in embryonic stem cells." Prog
Drug Res 2011 ; 67 : 239-52
27. Wu Q, Ng HH "Mark the transition: chromatin modifications and cell fate decision." Cell Res
2011 Oct ; 21(10) : 1388-90 Epub 2011 Sep 20
28. Gonzales KA, Ng HH "FoxO: a new addition to the ESC cartel." Cell Stem Cell 2011 Sep 2 ; 9(3)
: 181-3
29. Heng JC, Loh KM, Ng HH "Investigating the bona fide differentiation capacity of human
pluripotent stem cells." Cell Res 2011 Aug 30 Epub 2011 Aug 30
30. Xue K, Ng JH, Ng HH "Mapping the networks for pluripotency." s R Soc Lond B Biol Sci 2011
Aug 12 ; 366(1575) : 2238-46
31. Gonzales KA, Ng HH "Choreographing pluripotency and cell fate with transcription factors."
Biochim Biophys Acta 2011 Jul ; 1809(7) : 337-49 Epub 2011 Jun 21
32. Ng HH, Surani MA "The transcriptional and signalling networks of pluripotency." Nat Cell Biol
2011 May ; 13(5) : 490-6
33. Chia NY, Ng HH "Stem cell genome-to-systems biology." Wiley Interdiscip Rev Syst Biol Med
2011 Apr 11
34. Ang WX, Toh CX, Ng HH, Loh YH "Reproductive medicine gets a new tool." J Mol Cell Biol 2011
Dec ; 3(6) : 320-1 Epub 2011 Nov 24
35. Kunarso G, Chia NY, Jeyakani J, Hwang C, Lu X, Chan YS, Ng HH, Bourque G "Transposable
elements have rewired the core regulatory network of human embryonic stem cells." Nat Genet
2010 Jul ; 42(7) : 631-4 Epub 2010 Jun 6
36. Heng JC, Feng B, Han J, Jiang J, Kraus P, Ng JH, Orlov YL, Huss M, Yang L, Lufkin T, Lim B, Ng HH
"The nuclear receptor Nr5a2 can replace Oct4 in the reprogramming of murine somatic cells to
pluripotent cells." Cell Stem Cell 2010 Feb 5 ; 6(2) : 167-74 Epub 2010 Jan 21
37. Han J, Yuan P, Yang H, Zhang J, Soh BS, Li P, Lim SL, Cao S, Tay J, Orlov YL, Lufkin T, Ng HH,
Tam WL, Lim B "Tbx3 improves the germ-line competency of induced pluripotent stem cells."
Nature 2010 Feb 25 ; 463(7284) : 1096-100 Epub 2010 Feb 7
38. Agarwal S, Loh YH, McLoughlin EM, Huang J, Park IH, Miller JD, Huo H, Okuka M, Dos Reis RM,
Loewer S, Ng HH, Keefe DL, Goldman FD, Klingelhutz AJ, Liu L, Daley GQ "Telomere elongation in
induced pluripotent stem cells from dyskeratosis congenita patients." Nature 2010 Feb 17 Epub
2010 Feb 17
39. Heng JC, Ng HH "Transcriptional regulation in embryonic stem cells." Adv Exp Med Biol 2010 ;
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695 : 76-91
40. Ng JH, Ng HH "LincRNAs join the pluripotency alliance." Nat Genet 2010 Dec ; 42(12) : 1035-6
41. Heng JC, Orlov YL, Ng HH "Transcription Factors for the Modulation of Pluripotency and
Reprogramming." Cold Spring Harb Symp Quant Biol 2010 Nov 3
42. Chia NY, Chan YS, Feng B, Lu X, Orlov YL, Moreau D, Kumar P, Yang L, Jiang J, Lau MS, Huss M,
Soh BS, Kraus P, Li P, Lufkin T, Lim B, Clarke ND, Bard F, Ng HH "A genome-wide RNAi screen
reveals determinants of human embryonic stem cell identity." Nature 2010 Nov 11 ; 468(7321) :
316-20 Epub 2010 Oct 17
43. Xie D, Chen CC, Ptaszek LM, Xiao S, Cao X, Fang F, Ng HH, Lewin HA, Cowan C, Zhong S
"Rewirable gene regulatory networks in the preimplantation embryonic development of three
mammalian species." Genome Res 2010 Jun ; 20(6) : 804-15 Epub 2010 Mar 10
44. Satterlee JS, Schübeler D, Ng HH "Tackling the epigenome: challenges and opportunities for
collaboration." Nat Biotechnol 2010 Oct ; 28(10) : 1039-44
45. Im CN, Kang NY, Ha HH, Bi X, Lee JJ, Park SJ, Lee SY, Vendrell M, Kim YK, Lee JS, Li J, Ahn YH,
Feng B, Ng HH, Yun SW, Chang YT "A fluorescent rosamine compound selectively stains pluripotent
stem cells." Angew Chem Int Ed Engl 2010 Oct 4 ; 49(41) : 7497-500
46. Feng Bo, Ng Jia-Hui, Heng Jian-Chien Dominic, Ng Huck-Hui "Molecules that Promote or
Enhance Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells." Cell Stem Cell 2009
Apr 3 ; 4(4) : 301-12
47. Feng Bo, Jiang Jianming, Kraus Petra, Ng Jia-Hui, Heng Jian-Chien Dominic, Chan Yun-Shen, Yaw
Lai-Ping, Zhang Weiwei, Loh Yuin-Han, Han Jianyong, Vega Vinsensius B, Cacheux-Rataboul Valere,
Lim Bing, Lufkin Thomas, Ng Huck-Hui "Reprogramming of fibroblasts into induced pluripotent stem
cells with orphan nuclear receptor Esrrb." Nat. Cell Biol. 2009 Feb ; 11(2) : 197-203
48. Chan Ken Kwok-Keung, Zhang Jingyao, Chia Na-Yu, Chan Yun-Shen, Sim Hui Shan, Tan Ker Sin,
Oh Steve Kah-Weng, Ng Huck-Hui, Choo Andre Boon-Hwa "KLF4 and PBX1 Directly Regulate NANOG
Expression in Human Embryonic Stem Cells." Stem Cells 2009 Sep ; 27(9) : 2114-25
49. Yuan Ping, Han Jianyong, Guo Guoji, Orlov Yuriy L, Huss Mikael, Loh Yuin-Han, Yaw Lai-Ping,
Robson Paul, Lim Bing, Ng Huck-Hui "Eset partners with Oct4 to restrict extraembryonic trophoblast
lineage potential in embryonic stem cells." Genes Dev. 2009 Nov 1 ; 23(21) : 2507-20
50. He X, Chen CC, Hong F, Fang F, Sinha S, Ng HH, Zhong S "A biophysical model for analysis of
transcription factor interaction and binding site arrangement from genome-wide binding data."
PLoS One 2009 ; 4(12) : e8155 Epub 2009 Dec 1
51. Hong F, Fang F, He X, Cao X, Chipperfield H, Xie D, Wong WH, Ng HH, Zhong S "Dissecting
early differentially expressed genes in a mixture of differentiating embryonic stem cells." PLoS
Comput Biol 2009 Dec ; 5(12) : e1000607 Epub 2009 Dec 18
52. Lim Chin Yan, Tam Wai-Leong, Zhang Jinqiu, Ang Haw Siang, Jia Hui, Lipovich Leonard, Ng
Huck-Hui, Wei Chia-Lin, Sung Wing Kin, Robson Paul, Yang Henry, Lim Bing "Sall4 Regulates Distinct
Transcription Circuitries in Different Blastocyst-Derived Stem Cell Lineages." 2008 Sep 17 ; :
53. Chen X, Vega V B, Ng H-H "Transcriptional Regulatory Networks in Embryonic Stem Cells."
Cold Spring Harb. Symp. Quant. Biol. 2008 Nov 6 ; :
54. Xie Dan, Cai Jun, Chia Na-Yu, Ng Huck H, Zhong Sheng "Cross-species de novo identification of
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cis-regulatory modules with GibbsModule: application to gene regulation in embryonic stem cells."
Genome Res. 2008 Aug ; 18(8) : 1325-35
55. Tam Wai-Leong, Lim Chin Yan, Han Jianyong, Zhang Jinqiu, Ang Yen-Sin, Ng Huck-Hui, Yang
Henry, Lim Bing "T-cell factor 3 regulates embryonic stem cell pluripotency and self-renewal by the
transcriptional control of multiple lineage pathways." Stem Cells 2008 Aug ; 26(8) : 2019-31
56. Loh Yuin-Han, Ng Jia-Hui, Ng Huck-Hui "Molecular framework underlying pluripotency." Cell
Cycle 2008 Apr 1 ; 7(7) : 885-91
57. Jiang Jianming, Chan Yun-Shen, Loh Yuin-Han, Cai Jun, Tong Guo-Qing, Lim Ching-Aeng,
Robson Paul, Zhong Sheng, Ng Huck-Hui "A core Klf circuitry regulates self-renewal of embryonic
stem cells." Nat. Cell Biol. 2008 Mar ; 10(3) : 353-60
58. Tay Yvonne M-S, Tam Wai-Leong, Ang Yen-Sin, Gaughwin Philip M, Yang Henry, Wang Weijia,
Liu Rubing, George Joshy, Ng Huck-Hui, Perera Ranjan J, Lufkin Thomas, Rigoutsos Isidore, Thomson
Andrew M, Lim Bing "MicroRNA-134 modulates the differentiation of mouse embryonic stem cells,
where it causes post-transcriptional attenuation of Nanog and LRH1." Stem Cells 2008 Jan ; 26(1) :
17-29
59. Ng Jia-Hui, Heng Jian-Chien Dominic, Loh Yuin-Han, Ng Huck-Hui "Transcriptional and
epigenetic regulations of embryonic stem cells." Mutat. Res. 2008 Aug 20 ; :
60. Jiang Jianming, Ng Huck-Hui "TGFbeta and SMADs talk to NANOG in human embryonic stem
cells." 2008 Aug 7 ; 3(2) : 127-8
61. Chen Xi, Xu Han, Yuan Ping, Fang Fang, Huss Mikael, Vega Vinsensius B, Wong Eleanor, Orlov
Yuriy L, Zhang Weiwei, Jiang Jianming, Loh Yuin-Han, Yeo Hock Chuan, Yeo Zhen Xuan, Narang
Vipin, Govindarajan Kunde Ramamoorthy, Leong Bernard, Shahab Atif, Ruan Yijun, Bourque
Guillaume, Sung Wing-Kin, Clarke Neil D, Wei Chia-Lin, Ng Huck-Hui "Integration of external
signaling pathways with the core transcriptional network in embryonic stem cells." Cell 2008 Jun 13
; 133(6) : 1106-17
62. Chen Xi, Fang Fang, Liou Yih Cherng, Ng Huck Hui "Zfp143 regulates Nanog through
modulation of Oct4 binding." Stem Cells 2008 Aug 7 ; :
63. Bourque Guillaume, Leong Bernard, Vega Vinsensius B, Chen Xi, Lee Yen Ling, Srinivasan
Kandhadayar G, Chew Joon-Lin, Ruan Yijun, Wei Chia-Lin, Ng Huck Hui, Liu Edison T "Evolution of
the mammalian transcription factor binding repertoire via transposable elements." Genome Res.
2008 Oct 3 ; :
64. Zhao Xiao Dong, Han Xu, Chew Joon Lin, Liu Jun, Chiu Kuo Ping, Choo Andre, Orlov Yuriy L,
Sung Wing-Kin, Shahab Atif, Kuznetsov Vladimir A, Bourque Guillaume, Oh Steve, Ruan Yijun, Ng
Huck-Hui, Wei Chia-Lin "Whole-genome mapping of histone H3 Lys4 and 27 trimethylations reveals
distinct genomic compartments in human embryonic stem cells." 2007 Sep 13 ; 1(3) : 286-98
65. Loh Yuin-Han, Zhang Weiwei, Chen Xi, George Joshy, Ng Huck-Hui "Jmjd1a and Jmjd2c histone
H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells." Genes Dev. 2007 Oct 15 ;
21(20) : 2545-57
66. Lim Ching-Aeng, Yao Fei, Wong Joyce Jing-Yi, George Joshy, Xu Han, Chiu Kuo Ping, Sung
Wing-Kin, Lipovich Leonard, Vega Vinsensius B, Chen Joanne, Shahab Atif, Zhao Xiao Dong, Hibberd
Martin, Wei Chia-Lin, Lim Bing, Ng Huck-Hui, Ruan Yijun, Chin Keh-Chuang "Genome-wide mapping
of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon
TLR4 activation." Mol. Cell 2007 Aug 17 ; 27(4) : 622-35
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67. Vikhanskaya Faina, Toh Wen Hong, Dulloo Iqbal, Wu Qiang, Boominat han Lakshmanane, Ng
Huck Hui, Vousden Karen H, Sabapathy Kanaga "p73 supports cellular growth through
c-Jun-dependent AP-1 transactivation." Nat. Cell Biol. 2007 Jun ; 9(6) : 698-705
68. Lim Linda Shushan, Loh Yuin-Han, Zhang Weiwei, Li Yixun, Chen Xi, Wang Yinan, Bakre Manjiri,
Ng Huck-Hui, Stanton Lawrence W "Zic3 is required for maintenance of pluripotency in embryonic
stem cells." Mol. Biol. Cell 2007 Apr ; 18(4) : 1348-58
69. Yan Junli, Jiang Jianming, Lim Ching Aeng, Wu Qiang, Ng Huck-Hui, Chin Keh-Chuang "BLIMP1
regulates cell growth through repression of p53 transcription." Proc. Natl. Acad. Sci. U.S.A. 2007
Feb 6 ; 104(6) : 1841-6
70. Lim Ching-Aeng, Ng Huck-Hui "Application of advanced technologies in ageing research."
Mech. Ageing Dev. 2007 Jan ; 128(1) : 149-60
71. Wang Xueying, Kua Hui-Yi, Hu Yuanyu, Guo Ke, Zeng Qi, Wu Qiang, Ng Huck-Hui, Karsenty
Gerard, de Crombrugghe Benoit, Yeh James, Li Baojie "p53 functions as a negative regulator of
osteoblastogenesis, osteoblast-dependent osteoclastogenesis, and bone remodeling." J. Cell Biol.
2006 Jan 2 ; 172(1) : 115-25
72. Zhang Jinqiu, Tam Wai-Leong, Tong Guo Qing, Wu Qiang, Chan Hsiao-Yun, Soh Boon-Seng, Lou
Yuefei, Yang Jianchang, Ma Yupo, Chai Li, Ng Huck-Hui, Lufkin Thomas, Robson Paul, Lim Bing "Sall4
modulates embryonic stem cell pluripotency and early embryonic development by the
transcriptional regulation of Pou5f1." Nat. Cell Biol. 2006 Oct ; 8(10) : 1114-23
73. Wu Qiang, Chen Xi, Zhang Jinqiu, Loh Yuin-Han, Low Teck-Yew, Zhang Weiwei, Zhang
Wensheng, Sze Siu-Kwan, Lim Bing, Ng Huck-Hui "Sall4 interacts with Nanog and co-occupies
Nanog genomic sites in embryonic stem cells." J. Biol. Chem. 2006 Aug 25 ; 281(34) : 24090-4
74. Loh Yuin-Han, Wu Qiang, Chew Joon-Lin, Vega Vinsensius B, Zhang Weiwei, Chen Xi, Bourque
Guillaume, George Joshy, Leong Bernard, Liu Jun, Wong Kee-Yew, Sung Ken W, Lee Charlie W H,
Zhao Xiao-Dong, Chiu Kuo-Ping, Lipovich Leonard, Kuznetsov Vladimir A, Robson Paul, Stanton
Lawrence W, Wei Chia-Lin, Ruan Yijun, Lim Bing, Ng Huck-Hui "The Oct4 and Nanog transcription
network regulates pluripotency in mouse embryonic stem cells." Nat. Genet. 2006 Apr ; 38(4) :
431-40
75. Wei Chia-Lin, Wu Qiang, Vega Vinsensius B, Chiu Kuo Ping, Ng Patrick, Zhang Tao, Shahab Atif,
Yong How Choong, Fu YuTao, Weng Zhiping, Liu JianJun, Zhao Xiao Dong, Chew Joon-Lin, Lee Yen
Ling, Kuznetsov Vladimir A, Sung Wing-Kin, Miller Lance D, Lim Bing, Liu Edison T, Yu Qiang, Ng
Huck-Hui, Ruan Yijun "A global map of p53 transcription-factor binding sites in the human genome."
Cell 2006 Jan 13 ; 124(1) : 207-19
76. Chew Joon-Lin, Loh Yuin-Han, Zhang Wensheng, Chen Xi, Tam Wai-Leong, Yeap Leng-Siew, Li
Pin, Ang Yen-Sin, Lim Bing, Robson Paul, Ng Huck-Hui "Reciprocal transcriptional regulation of
Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells." Mol. Cell. Biol. 2005 Jul ;
25(14) : 6031-46
77. Rodda David J, Chew Joon-Lin, Lim Leng-Hiong, Loh Yuin-Han, Wang Bei, Ng Huck-Hui, Robson
Paul "Transcriptional regulation of nanog by OCT4 and SOX2." J. Biol. Chem. 2005 Jul 1 ; 280(26) :
24731-7
78. Kho PS, Wang Z, Zhuang L, Li Y, Chew JL, Huck Hui NG, Edison Tak-Bun LIU, Qiang YU (2004)
"p53-regulated transcriptional program associated with genotoxic stress-induced apoptosis" J Biol
Chem 279 21183
79. Yijun RUAN, Le Ber P, Huck Hui NG, Edison Tak-Bun LIU (2004) "Interrogating the
7/9
transcriptome" Trends in Biotechnology 22(1) 23-30
80. Nicolas O. Fortunel, Hasan Otu, Huck Hui NG, Chen, J., Mu, X., Chevassut, T., Li, X., Joseph M.,
Bailey C., Hatzfeld, J., Usta, F., Vinsensius Berlian VEGA, Long M. Philip, Towia Liberman, T., Bing
LIM (2003) "Comment on `Stemness: Transcriptional Profiling of Embryonic and Adult Stem Cells'
and `A Stem Cell Molecular Signature'" Science 302 393
81. Huck Hui NG, Ciccone, DN, Morshead, KB, Oettinger, MA, Struhl, K (2003) "Lysine 79 of histone
H3 is hypomethylated at silenced loci in yeast and mammalian cells: a potential mechanism for
maintaining epigenetic states" Proc. Natl. Acad. Sci. (U.S.A) In press
82. Huck Hui NG, Robert, F, Young, RA, Struhl, K (2003) "Targeted recruitment of Set1 histone
methylase by elongating Pol II provides a localized mark and memory of recent transcriptional
activity" Molecular Cell In press
83. Huck Hui NG, Dole, S, Struhl, K. (2003) "The Rtf1 component of the Paf1 transcriptional
elongation complex, is required for ubiquitination of histone H2B" J. Biol. Chem. (Accelerated
Publication) 278 33625-33628
84. Cawley, S, Bekiranov, S, Huck Hui NG, Kapranov, P, Sekinger, E, Kampa, D., Piccolboni, A.,
Sementchenko, V., Cheng, J., Williams, AJ, Wheeler, R, Wong, B, Drenkow, J, Yamanaka, M, Patel, S,
Brubaker, S, Tammana, H, Helt, G, Struhl, K, Gingeras, TR (2003) "Unbiased Mapping of
Transcription Factor Binding Sites Along Human Chromosomes 21 and 22 Points to Widespread
Regulation of Non-Coding RNAs" Cell 116 499-509
85. Feng, Q, Wang, H, Huck Hui NG, Erdjument-Bromage, H, Tempst, P, Struhl, K, Zhang, Y (2002)
"Methylation of H3-Lysine 79 Is Mediated by a New Family of HMTases without a SET Domain" Curr.
Biol. 12, 1052-1058
86. Huck Hui NG, Feng, Q, Wang, H, Erdjument-Bromage, H, Tempst, P, Zhang, Y, Struhl, K (2002)
"Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric
silencing and Sir protein association" Genes Dev 16, 1518-1527
87. Huck Hui NG, Robert, F, Young, RA, Struhl, K (2002) "Genome wide location and regulated
recruitment of the RSC nucleosome remodeling complex" Genes Dev 16, 806-19
88. Huck Hui NG, Xu, RM, Zhang, Y, Struhl, K (2002) "Ubiquitination of histone H2B by Rad6 is
required for efficient Dot1-mediated methylation of histone H3-lysine 79" J. Biol. Chem.
(Accelerated Publication) 277, 34655-34657
89. Huck Hui NG (2000) "Histone deacetylases: silencers for hire" Trends Biochem. Sci. 25,
121-126
90. Huck Hui NG, Bird, A. (2000) "Functional characterisation of the methyl-CpG binding protein
MBDI" Mol. Cell. Biol 20, 1394-1406
91. Huck Hui NG, Erdjument_Bromage H, Bird, A. (1999) "Analysis of the NuRD subunits reveals a
histone deacetylase core complex and a connection with DNA methylation." Genes Dev 13,
1924-1935
92. Huck Hui NG, Jiricny, J, Bird, A (1999) "MBD4 is a novel DNA thymine glycosylase that can bind
to the product of deamination at methylated CpG" Nature 401, 301-304
93. Huck Hui NG, Hendrich, B, Johnson, CA, Turner, BM, Erdjument-Bromage, H, Tempst, P,
Reinberg, D, Bird, A (1999) "The methyl-CpG binding protein MBD2 is a transcriptional repressor
belonging to the MeCP1 fraction of histone deacetylase complexes" Nature Genetics 23, 58-61
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94. Huck Hui NG, Barlow, AL, Turner, BM.,, Hendrich, B, Bird, A (1999) "Vestiges of a DNA
methylation system in Drosophila melanogaster?" Nature Genetics 23, 389-390
95. Huck Hui NG (1999) "DNA methylation and chromatin modification" Curr. Opin. Genet. Dev. 9,
158-163
96. Huck Hui NG, Johnson, CA, Laherty, CD, Turner, BM, Eisenman, RN, Bird, A (1998)
"Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone
deacetylase complex" Nature. 393, 386-389 * Ng Huuck Hui & Nan X. are equal first authors
97. Koh, TW, Huck Hui NG, Xu, G, Li, BF (1997) "Human DNA-(cytosine-5) methyltransferase-PCNA
complex as a target for p21WAF1" Science 277, 1996-2000
98. Huck Hui NG, Chia, JN, Li, BF. (1996) "The characterisation of independent DNA- and multiple
Zn-binding domains at the N-terminus of human DNA (cytosine-5)-methyltransferase: modulating
the activity of an independent DNA-binding domain by" J. Mol. Biol 257, 935-948
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