Supplementary figures
The effect of immunosuppressive drug cyclosporine A on myeloid-derived suppressor cells in
transplanted mice
Chenlu Han1,2,#, Tingting Wu1,#, Ning Na3, Yang Zhao1, Weiguo Li2,*, and Yong Zhao1,*
1 College of Life Science, Henan Normal University, Xinxiang, Henan, China; 2 Transplantation Biology
Research Division, State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of
Sciences, Beijing, China. 3 Department of Kidney Transplantation, The Third Affiliated Hospital of Sun Yatsen University, Guangzhou, Guangdong, China
3.5
30
35
35
3
25
30
30
2.5
15
2
1.5
1
10
% of CD274
20
25
20
% of CD115
25
% of I-Ab
%of CD31
40
15
10
20
15
10
5
0.5
5
5
0
0
0
0
Control
CsA
Control CsA
Control
CsA
Control
CsA
Supplementary Figure 1. CsA treatment did not alter the phenotype of MDSCs.
Bone marrow cells were cultured in GM-CSF or GM-CSF+CsA as described in Materials and Methods for 4
days. The expression of CD31, I-Ab, CD115 and CD274 in the gated CD11b+Gr1+ cells were analyzed by
FCM .Data was shown as mean ± SD (n = 3/group), which represent one of two independent experiments
with similar results..
Relative expression of mRNA
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Control
CsA
Supplementary Figure 2. The mRNA expression of Arg1, iNOS, TNF-α, TGF-β1, IDO, IL-10, IL-12, COX2, NOX2
and HO-1 in GM-CSF or GM-CSF+CsA-induced MDSCs were determined by real-time PCR.
Bone marrow cells were cultured in GM-CSF or GM-CSF+CsA as described in Materials and Methods for 4 days .The
gene expressions of Arg1, iNOS, TNF-α, TGF-β1, IDO, IL-10, IL-12, COX2, NOX2 and HO-1 were determined by realtime PCR. Data were shown as mean ± SD (n = 3/group), which represent one of two independent experiments with
similar results..