Fig. S1. Human embryonic epidermis contains a CD14+ leukocyte subset. Immunoflourescence triple labelling identified
CD45+CD36+CD14+ (arrowhead) and CD45+CD36-CD14- (double arrow) epidermal cells in first trimester human skin. LC in healthy
adult skin do not express CD14. Scale bars: 50 µm.
Fig. S2. Some CD36+ leukocytes coexpress the dendritic cell marker CD1c. Immunofluorescence triple labelling identified
CD45+CD36+CD1c+ (arrow) and CD45+CD36-CD1c+ (arrowhead) epidermal cells in first trimester human skin. LC in healthy adult skin
express CD1c but never CD36. Scale bars: 50 µm.
Fig. S3. Resident epidermal LC precursors do not express CD34. Immunoflourescence double labeling identified solitary (middle
column, arrowhead) and vessel-associated (right column, inset) CD45+CD34+ dermal cells. Epidermal leukocytes do not express CD34
(arrow). Scale bars: 50 µm.