Development 142: doi:10.1242/dev.128215: Supplementary information
Figure S1. huC regulatory sequences drive expression in some OPCs. Tg:olig2:GFP
stably transgenic embryos were injected with a construct encoding huC:mCherry-CAAX,
which labeled cells expressing huC regulatory sequences with membrane-tethered
mCherry (B, red). (A-C) Representative embryo showing (A) GFP in oligodendrocytes
and motor neurons, (B) membrane tethered mCherry, and (C) merged image. OPCs and
motor neurons were distinguished by morphology and position. In 3 of 38 injected
embryos, some OPCs co-expressed GFP and membrane-tethered mCherry (arrow),
indicating that the HuC construct may be expressed in a subset of cells of the
in A-C.
Development • Supplementary information
oligodendrocyte lineage. (A’-C’) These images show magnified views of boxed regions
Development 142: doi:10.1242/dev.128215: Supplementary information
Movie 1. Time-lapse imaging and tracking of cells expressing Tg:olig2:GFP in
wildtype at 48-52 hpf. At this stage, the first few OPCs began to exit the midline. 4
Movie 2. Time-lapse imaging of cells expressing Tg:olig2:GFP in znf16l mutant at
48-52 hpf. No OPC migration was observed in the mutant at this stage. 4 hours in real
time; 1 image taken every 3 min; playback at 10fps.
Development • Supplementary information
hours in real time; 1 image taken every 3 min; playback at 10fps.
Development 142: doi:10.1242/dev.128215: Supplementary information
Movie 3. Time-lapse imaging and tracking of cells expressing Tg:olig2:GFP in
wildtype at 64-72 hpf. 8 hours in real time; 1 image taken every 3 min; playback at
Movie 4. Time-lapse imaging of cells expressing Tg:olig2:GFP in znf16l mutant at
64-72 hpf. At this stage, mutants began their recovery and the first few OPCs exited the
midline to colonize the midbrain and hindbrain. 8 hours in real time; 1 image taken every
3 min; playback at 10fps.
Development • Supplementary information
10fps.