CellASIC ONIX™ System Setup Guide
1.
2.
3.
4.
5.
System Components
Required Equipment
System Set-Up
CO2 Calibration
Temperature Calibration
7. Microscope Set-Up
8. Microincubation
9. Software
10. Culture and Image Cells
1. System Components
ONIX Flow System
and Manifold
Bioptechs Inc.
Objective Heater
and Controller
BIOPTECH
Setpoint
Alarm
Reset
Objective
Reference
Objective Heater Controller
Select
S
TECH
BIOP
Caution!
-Do not remove the heater from the objective if the controller is on.
- Always press [Alarm Reset] before plugging the heater into the controller.
-Plug the heater in to the controller after the heater is installed onto the objective.
Made in U.S.A.
C
PTE
BIO
HS
Temperature
Calibration Plate
CO2 sensor
The CellASIC ONIX™ platform is sold with the components displayed above.
Software
2. Required Equipment
Computer
Inverted Microscope
100% CO2 gas tank
3. System Set-up
To Power
Supply
To Computer
To 100%
Carbon Dioxide
enter a desired protocol.
4. CO2 Calibration
1. Ensure the CO2 source is
between 10-40 psi...
2. Connect tubing adaptor input from CO2 sensor
input into leftmost Leur fitting on system.
... and connected
to rear input of the
ONIX flow system.
3. Power on ONIX system and flip on
CO2/Air switch.
4. Check display for CO2 percentage. Use a small
flathead screwdriver to adjust CO2 regulator on
the back of the system to desired output.
Optional: If the user wishes to monitor the CO2 levels during an experiment, the CO2 sensor output can be connected to the
manifold with the provided tubing.
5. Temperature Calibration
1. Mount Objective Heater and
add a drop of immersion oil to the
objective.
4. Find and focus on culture
chamber with microscope.
2. Seal manifold to temperature
calibration plate.
5. Connect thermistor cord to
reference socket on heater
controller.
3. Place plate on microscope while
threading thermistor cord under
the stage.
6. Adjust Setpoint to obtain
desired temperature.
The temperature calibration plate plugs into the objective heater controller. Its unique design allows for precise temperature
calibration of the cell culture chamber.
6. Microfluidic Plate and Manifold
1. Attach manifold to ONIX
system with Luer fittings.
4. Flip Vacuum switch on.
2. Pipette solutions into
microfluidic plate.
3. Align manifold cover
with plate.
5. Push down on manifold.
6. Sealed light will turn on
indicating proper closure.
Push Down
The manifold delivers both positive and negative pressures to each well to control fluid flow through the cell chamber. It also
provides a sterile environment for the cells and serves as a conduit for CO2 delivery.
7. Microscope Set-Up
To ONIX Flow
System
Objective
Heater
Manifold Cover and
Microfluidic Plate
To Bioptechs Inc.
Objective Heater
Controller
Place the Bioptechs objective heater onto the objective lens. Plug the heater ino the heater controller to view set temperature.The
objective heater maintains a stable temperature throughout the duration of your experiment. Place plate and manifold together
onto the microscope stage for live cell imaging.
8. Microincubation
side view
Manifold
5% CO2
Cells at 37˚
Glass coverslip
Heated objective lens
top view
-
ture.
9. Software
Flow Rate Setting
Status Indicators
Individual Well
Controls
Program Timer
ONIX FG software program automates your perfusion protocol for automated solution switching. The program allows the user to
10. Culture and Image Cells
PC-3 cells stained with DAPI, phalloidin, and an anti-alpha-tubulin marker
The ONIX™is compatible with a wide variety of bacterial, yeast, and mammalian cell types. The system is adaptable to any
perfusion-base experiment.
CellASIC Corporation • 2551 Merced Street • San Leandro, CA, 94577 • (866) 316-1259 • www.cellasic.com