Figure S2. (A) Phase-contrast image of differentiating EBs under SC conditions and RCCS at
day6.
mESCs line is HCM1. Bar=200 m. (B) mRNA levels of pluripotent genes in SC
gourp EBs or RCCS group
EBs were assessed by normal RT-PCR. GAPDH was employed
as a loading control. (C) mRNA levels of germ layer genes in SC gourp’s EBs or RCCS
group’s
EBs assessed by normal RT-PCR. GAPDH was employed as a loading control.