DPG
PL2
PG
PG
PAL
PC
PE
PL1
PI PGL
Molybdenum blue
GL2
L1
PL
DPG
2
PE
PG
PG
GL2
PALPC PL1
GL1
PI
PGL GL1
Molybdate phosphoric acid
GL2 L1
DPGPL2
PE
PG
PG
L3
PC
PL1 GL2
PAL
GL1 L4
PI
GL
PGL 1
Anisaldehyde
Fig. S1 Two-dimensional thin-layer chromatogram stained with molybdenum blue, molybdate
phosphoric acid and anisaldehyde reagents for polar lipids of H195T (DSM 46680T). The
chromatographic conditions were as follows. Silica Gel 60 thin-layer plates (10 by 10 cm) were
spotted with 15 μl of a whole-cell lipid extract
Chloroform/methanol/water (65:25:4, by vol) was used in the first direction, and chloroform/acetic
acid/methanol/water (80:18:12:5, by vol) was used in the second direction. Plates were sprayed
with different reagents to locate and differentiate the polar lipids
Abbreviations: DPG: diphosphatidylglycerol; PG: phosphatidylglycerol; PC: phosphatidylcholine;
PI: phosphatidylinositol; L: unknown lipid (1x). GL: unknown glycolipid (2x).