Supplementary Figure 1 PCR analysis for cadA deletion, thrBC deletion and gdhA
integration.
Site thrBC: MG1655 (wild-type thrBC site), 2.8 kb; intermediate strain (thrBC:cm), 2.2 kb;
final strain (ΔthrBC), 0.9kb
Site cadA: MG1655 (wild-type cadA), 1.9 kb; intermediate strain (cadA::apr), 2.4 kb; final
strain (ΔcadA), 0.9 kb.
Site gntT: MG1655 (wild-type gntT ), 1.1 kb; intermediate strain (spectinomycin-resistance
gene integration), 2.5 kb; final strain (gdhA integration), 2.8 kb.