Okwong’ Oketch Reymick Department of Science, Technical and Vocational Education (DSTVE), School of Education, College of Education and External Studies Makerere University P.O BOX, 7062 Kampala, Uganda +256773 313181, +256 753 313181 [email protected] A. Profile: I am an extremely focused, honest, dedicated, trust worthy, enthusiastic and self-sustaining individual with first class interpersonal and communication skills at all levels. I can work for long hours, take extra work loads/tasks and perform my duties under minimum supervision. I have excellent research and practical skills in educational and medical research and can quickly translate a concept into a research proposal. With my good management and organizational skills, I can promote group work, create constructive friendship necessary for the success of any organization. B. Work experience Year Company/Organization Title and other Responsibilities 2009 Makerere University, 1. Lecturer; lecturing Cell biology, Genetics, to College of Education & Virology, Immunology and Biology Teaching date External Studies Methods (CEES), 2. Coordinator of Biology unit and Team leader for School of Education the Cell biology/ Genetics/Microbiology sub-unit. (SOE), Coordinator of the Physical Education unit Department of Science, 3. Coordinator of exams and students results at Technical & Vocational DSTVE. Education (DSTVE) 4. DSTVE representative to the Examinations and irregularities board of CEES 5. College representative to estates 2009 Molecular biology 1. Research assistant; Preparation of VSG from to laboratory Trypanosoma brucei brucei, Production of anti-VSG 2010 antibodies in rabbits, immunolocalization of T. b. Okwong’ Oketch Reymick 1 (What men have done, man can do!) brucei in cattle brain tissue 2009 King’s College, Budo Subject teacher; teaching biology and Chemistry at to O-level and A-level date Class teacher: Form 5 (Biology class, 2012), Form 6 (Biology class, 2013) 2006 Mengo Senior School to Subject teacher; teaching biology and Chemistry at O-level and A-level 2009 ……………………………………………………………………………………………… C. Professional Membership and belonging Makerere University Academic Staff Association (MUASA) Uganda National Teachers Union (UNATU) D. Education and Qualifications YEAR INSTITUTION QUALIFICATION 2008 to 2011 Makerere University, Master of Science in Molecular Biology and Faculty of Veterinary Biotechnology Medicine. Research topic: Developing anti-variant surface glycoprotein antibodies for the Immunostaining of heterologous strains of Trypanosoma brucei in situ and in vitro Supervisors: 1. Prof. G. W. Lubega 2. Dr. Nanteza Ann Date of Graduation: 22nd January, 2013 2003 to 2007 Makerere University, Bachelor of Science with education, School of Education. Biology/Chemistry Date of Graduation: 22nd January, 2007 2001 to 2002 Makerere High School, Uganda Advanced Certificate of Education Kampala (UACE). Okwong’ Oketch Reymick 2 (What men have done, man can do!) 1996 to 2000 Duhaga Secondary Uganda Certificate of Education (UCE) School, Hoima 1988 to 1995 Kalkada Primary Kenya Certificate for Primary Education School, Siaya (KCPE) E. Trainings undertaken PERIOD/ TRAINER AREA(S) TRAINED IN Molecular laboratory at Population genetics for farm animal MUIENR (Organized by Dr. conservation DATE 31/01/2013 Masembe), Makerere University July, 2012 Publication and Proposal writing Directorate of Graduate Training, Makerere University 2009/2010 Molecular biology laboratory, Molecular biology techniques Faculty of Veterinary including Cell counting, Gene Medicine, Makerere University knockout, DNA sequencing techniques (theoretically) Column chromatography, Enzyme-linked Immunosorbent Assay (ELISA), Immunofluorescence Assay, Polymerase chain reaction, Southern blot, Western blot analysis, gel electrophoresis Protein work including preparation of VSG from trypanosome surface, quantification of protein, Dialysis of proteins, Reconstitution of proteins. Immunization experiments including Okwong’ Oketch Reymick 3 (What men have done, man can do!) preparation of immunogen from proteins, immunization of rabbits and rats, collection of sera from rabbits, storage of proteins and sera. 2009 HIV Reference laboratory, ELISA technique for the diagnosis of Uganda virus Research HIV Institute Entebbe 2009, 2010, Uganda National 2011 Examinations Board Marking and Setting A-level Biology F. Research Experience in Medical Research Practical Skill 1: Biochemical Methods – Proteins work Level of Expertise: Description: Advanced I Personally did the following in the research that led to award of MSc in Molecular Biology and Biotechnology of Makerere University Kampala, being supervised by Prof. G. W. Lubega (contact details on the last page of this CV as a referee) i. Prepared Variant Surface Glycoprotein (VSG) from the surface of Trypanosoma brucei brucei UTRO 010291B and analyzed it by sodium dodecyl sulfatepolyacrylamide gel electrophoresis. ii. Dialysed VSG and later reconstituted it. iii. Quantified VSG using the Bradford assay for the quantification of proteins iv. Prepared an immunogen (VSG + Phosphate buffered Saline + Freunds v. Adjuvants) and used the immunogen to immunize rabbits. vi. From the rabbits I collected serum which I analyzed by Western blotting to monitor development of antibodies. vii. Using the antibodies, I carried out immunofluorescence assay (IFA) on blood stream form and procyclic form Trypanosoma brucei brucei UTRO 010291B (which I had grown in a culture medium). I also performed IFA on cattle brain sections. Practical Skill 2: Genetics/Genetic Screens Level of Expertise: Basic Okwong’ Oketch Reymick 4 (What men have done, man can do!) Description: i. ii. iii. Polymerase Chain Reaction: used it to amplify portions of bacterial and Trypanosome DNA Electrophoresis: separated proteins by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Also separated DNA by Agarose gel electrophoresis. Cloning: ligated fragments of trypanosome DNA into plasmids, transfected the plasmids into bacterial cells and expressed the proteins in the bacteria Practical Skill 3: Immunological Methods Level of Expertise: Description: Advanced Produced anti-variant surface glycoprotein antibodies in rabbits and used immunofluorescence assay to detect trypanosomes in brain sections of cattle and also to detect the trypanosomes from a culture medium Practical Skill Virology Methods Level of Expertise Basic Description: Performed ELISA for the detection of HIV 1 and HIV 2 at the HIV Reference Laboratory, Uganda Virus Research Institute. G. Key activities undertaken Date/Period Activity Organizer Thursday Uwezo East Africa Annual learning Uwezo East Africa- Thru. 8 May, Assessment report launch Uwezo Uganda-Country 2014 (Representing Dean, SOE) Coordinator; th Mary Goretti Nakabugo (http://www.uwezo.net/) Friday 2014 National Assessment of Progress in UNEB 4th April, Education (NAPE) Feedback Workshop Executive Director 2014 (Representing Dean, SOE) Matthew Bukenya Thursday Infectious Disease Institute (IDI) Open Executive Director- IDI 20th Feb, Day (At IDI Mulago) (Alex Coutinho) 2014 (Representing Dean, SOE) Okwong’ Oketch Reymick 5 (What men have done, man can do!) 29/01/2013 Staff Development Workshop King’s College Budo October, Course harmonization at Makerere Makerere University 2011 University – Member of the Genetics and Senate Biotechnology sub-committee July-Sept Review of Cell Biology, Genetics & 2011 Evolution course units for the Bachelor of Technical & Vocational Department of Science, Science with Education of Makerere Education, Makerere University, Kampala. University, Kampala. 04/04/2011- 2nd Monitoring of teaching of Physical Ministry of Education & 27/04/2011 Education in secondary schools in Sports, department of Uganda - districts of Mukono, Jinja, Physical Education Iganga, Kaliro & Kamuli 22/11/2010- Monitoring the teaching of Physical Ministry of Education & 27/11/2010 Education in secondary schools in Sports, department of Uganda -districts of Bushenyi, Mbarara, Physical Education Kiruhura, Sheema & Buhweju H. Skills obtained 1. Proposal and report writing 2. Research work in Medical Research (Immunology) and Educational research 3. Laboratory work in Molecular Biology techniques, and secondary school Biology and Chemistry (Physical, Organic and Inorganic) practicals 4. Administrative skills 5. Teaching I. Interests 1. Medical Research work and other Laboratory work 2. Educational Research in Biology 3. Sports (football player) Okwong’ Oketch Reymick 6 (What men have done, man can do!) 4. Reading publications particularly in Medical research 5. Attending seminars, workshops etc J. REFEREES. 1. Dr. Henry Busulwa Head, Department of Science Technical & Vocational Education (DSTVE) School of Education, College of Education and External Studies Makerere University Mob : +256701695726 E-mail : [email protected] 2. Dr. Betty Ezati Dean, SOE Makerere University 3. Prof. George William Lubega Senior researcher, Molecular Biology Laboratory, Course coordinator, MSc (Molecular Biology and Biotechnology) Senior Lecturer, Department of Biomolecular Resources and Biolab Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University. Mob : +256772708800 E-mail: [email protected] 4. Dr. Anthoni Muwagga Mugagga Deputy Principle, CEES Makerere University Okwong’ Oketch Reymick 7 (What men have done, man can do!)
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