CV_Oketch_Reymick.pdf

Okwong’ Oketch Reymick
Department of Science, Technical and Vocational Education (DSTVE),
School of Education, College of Education and External Studies
Makerere University
P.O BOX, 7062 Kampala, Uganda
+256773 313181, +256 753 313181
[email protected]
A. Profile: I am an extremely focused, honest, dedicated, trust worthy, enthusiastic
and self-sustaining individual with first class interpersonal and communication
skills at all levels. I can work for long hours, take extra work loads/tasks and
perform my duties under minimum supervision. I have excellent research and
practical skills in educational and medical research and can quickly translate a
concept into a research proposal. With my good management and organizational
skills, I can promote group work, create constructive friendship necessary for the
success of any organization.
B. Work experience
Year
Company/Organization Title and other Responsibilities
2009
Makerere University,
1. Lecturer; lecturing Cell biology, Genetics,
to
College of Education &
Virology, Immunology and Biology Teaching
date
External Studies
Methods
(CEES),
2. Coordinator of Biology unit and Team leader for
School of Education
the Cell biology/ Genetics/Microbiology sub-unit.
(SOE),
Coordinator of the Physical Education unit
Department of Science,
3. Coordinator of exams and students results at
Technical & Vocational
DSTVE.
Education (DSTVE)
4. DSTVE representative to the Examinations and
irregularities board of CEES
5. College representative to estates
2009
Molecular biology
1. Research assistant; Preparation of VSG from
to
laboratory
Trypanosoma brucei brucei, Production of anti-VSG
2010
antibodies in rabbits, immunolocalization of T. b.
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brucei in cattle brain tissue
2009
King’s College, Budo
Subject teacher; teaching biology and Chemistry at
to
O-level and A-level
date
Class teacher: Form 5 (Biology class, 2012), Form 6
(Biology class, 2013)
2006
Mengo Senior School
to
Subject teacher; teaching biology and Chemistry at
O-level and A-level
2009
………………………………………………………………………………………………
C. Professional Membership and belonging
Makerere University Academic Staff Association (MUASA)
Uganda National Teachers Union (UNATU)
D. Education and Qualifications
YEAR
INSTITUTION
QUALIFICATION
2008 to 2011
Makerere University,
Master of Science in Molecular Biology and
Faculty of Veterinary
Biotechnology
Medicine.
Research topic: Developing anti-variant
surface glycoprotein antibodies for the
Immunostaining of heterologous strains of
Trypanosoma brucei in situ and in vitro
Supervisors: 1. Prof. G. W. Lubega
2. Dr. Nanteza Ann
Date of Graduation: 22nd January, 2013
2003 to 2007
Makerere University,
Bachelor of Science with education,
School of Education.
Biology/Chemistry
Date of Graduation: 22nd January, 2007
2001 to 2002
Makerere High School,
Uganda Advanced Certificate of Education
Kampala
(UACE).
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1996 to 2000
Duhaga Secondary
Uganda Certificate of Education (UCE)
School, Hoima
1988 to 1995
Kalkada Primary
Kenya Certificate for Primary Education
School, Siaya
(KCPE)
E. Trainings undertaken
PERIOD/
TRAINER
AREA(S) TRAINED IN
Molecular laboratory at
Population genetics for farm animal
MUIENR (Organized by Dr.
conservation
DATE
31/01/2013
Masembe), Makerere
University
July, 2012
Publication and Proposal writing
Directorate of Graduate
Training, Makerere University
2009/2010
Molecular biology laboratory,
Molecular biology techniques
Faculty of Veterinary
including Cell counting, Gene
Medicine, Makerere University knockout, DNA sequencing techniques
(theoretically) Column
chromatography, Enzyme-linked
Immunosorbent Assay (ELISA),
Immunofluorescence Assay,
Polymerase chain reaction, Southern
blot, Western blot analysis, gel
electrophoresis
Protein work including preparation of
VSG from trypanosome surface,
quantification of protein, Dialysis of
proteins, Reconstitution of proteins.
Immunization experiments including
Okwong’ Oketch Reymick
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preparation of immunogen from
proteins, immunization of rabbits and
rats, collection of sera from rabbits,
storage of proteins and sera.
2009
HIV Reference laboratory,
ELISA technique for the diagnosis of
Uganda virus Research
HIV
Institute Entebbe
2009, 2010,
Uganda National
2011
Examinations Board
Marking and Setting A-level Biology
F. Research Experience in Medical Research
Practical Skill 1:
Biochemical Methods – Proteins work
Level of Expertise:
Description:
Advanced
I Personally did the following in the research that led to award of MSc in Molecular
Biology and Biotechnology of Makerere University Kampala, being supervised by
Prof. G. W. Lubega (contact details on the last page of this CV as a referee)
i.
Prepared Variant Surface Glycoprotein (VSG) from the surface of Trypanosoma
brucei brucei UTRO 010291B and analyzed it by sodium dodecyl sulfatepolyacrylamide gel electrophoresis.
ii. Dialysed VSG and later reconstituted it.
iii. Quantified VSG using the Bradford assay for the quantification of proteins
iv.
Prepared an immunogen (VSG + Phosphate buffered Saline + Freunds
v. Adjuvants) and used the immunogen to immunize rabbits.
vi.
From the rabbits I collected serum which I analyzed by Western
blotting to monitor development of antibodies.
vii.
Using the antibodies, I carried out immunofluorescence assay (IFA) on blood
stream form and procyclic form Trypanosoma brucei brucei UTRO 010291B
(which I had grown in a culture medium). I also performed IFA on cattle brain
sections.
Practical Skill 2:
Genetics/Genetic Screens
Level of Expertise:
Basic
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Description:
i.
ii.
iii.
Polymerase Chain Reaction: used it to amplify portions of bacterial and
Trypanosome DNA
Electrophoresis: separated proteins by Sodium dodecyl sulfate-polyacrylamide
gel electrophoresis. Also separated DNA by Agarose gel electrophoresis.
Cloning: ligated fragments of trypanosome DNA into plasmids, transfected the
plasmids into bacterial cells and expressed the proteins in the bacteria
Practical Skill 3:
Immunological Methods
Level of Expertise:
Description:
Advanced
Produced anti-variant surface glycoprotein antibodies in rabbits and used
immunofluorescence assay to detect trypanosomes in brain sections of cattle and also to
detect the trypanosomes from a culture medium
Practical Skill
Virology Methods
Level of Expertise
Basic
Description:
Performed ELISA for the detection of HIV 1 and HIV 2 at the HIV Reference
Laboratory, Uganda Virus Research Institute.
G. Key activities undertaken
Date/Period
Activity
Organizer
Thursday
Uwezo East Africa Annual learning
Uwezo East Africa- Thru.
8 May,
Assessment report launch
Uwezo Uganda-Country
2014
(Representing Dean, SOE)
Coordinator;
th
Mary Goretti Nakabugo
(http://www.uwezo.net/)
Friday
2014 National Assessment of Progress in
UNEB
4th April,
Education (NAPE) Feedback Workshop
Executive Director
2014
(Representing Dean, SOE)
Matthew Bukenya
Thursday
Infectious Disease Institute (IDI) Open
Executive Director- IDI
20th Feb,
Day (At IDI Mulago)
(Alex Coutinho)
2014
(Representing Dean, SOE)
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29/01/2013
Staff Development Workshop
King’s College Budo
October,
Course harmonization at Makerere
Makerere University
2011
University – Member of the Genetics and
Senate
Biotechnology sub-committee
July-Sept
Review of Cell Biology, Genetics &
2011
Evolution course units for the Bachelor of Technical & Vocational
Department of Science,
Science with Education of Makerere
Education, Makerere
University, Kampala.
University, Kampala.
04/04/2011-
2nd Monitoring of teaching of Physical
Ministry of Education &
27/04/2011
Education in secondary schools in
Sports, department of
Uganda - districts of Mukono, Jinja,
Physical Education
Iganga, Kaliro & Kamuli
22/11/2010-
Monitoring the teaching of Physical
Ministry of Education &
27/11/2010
Education in secondary schools in
Sports, department of
Uganda -districts of Bushenyi, Mbarara,
Physical Education
Kiruhura, Sheema & Buhweju
H. Skills obtained
1. Proposal and report writing
2. Research work in Medical Research (Immunology) and Educational research
3. Laboratory work in Molecular Biology techniques, and secondary school Biology
and Chemistry (Physical, Organic and Inorganic) practicals
4. Administrative skills
5. Teaching
I. Interests
1. Medical Research work and other Laboratory work
2. Educational Research in Biology
3. Sports (football player)
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4. Reading publications particularly in Medical research
5. Attending seminars, workshops etc
J. REFEREES.
1. Dr. Henry Busulwa
Head, Department of Science Technical & Vocational Education (DSTVE)
School of Education, College of Education and External Studies
Makerere University
Mob : +256701695726
E-mail : [email protected]
2. Dr. Betty Ezati
Dean, SOE
Makerere University
3. Prof. George William Lubega
Senior researcher, Molecular Biology Laboratory,
Course coordinator, MSc (Molecular Biology and Biotechnology)
Senior Lecturer, Department of Biomolecular Resources and Biolab Sciences,
College of Veterinary Medicine, Animal Resources and Biosecurity,
Makerere University.
Mob : +256772708800
E-mail: [email protected]
4. Dr. Anthoni Muwagga Mugagga
Deputy Principle, CEES
Makerere University
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(What men have done, man can do!)