LIFE SCIENCE TECHNOLOGIES
Produced by the Science/AAAS Custom Publishing Office
NEW PRODUCTS: GENOMICS
Genomic Editing Tool
CRISPR/Cas9 is revolutionizing the field
of genomic editing by providing scientists with a powerful tool able to change
any gene, in any cell in a highly targeted
manner and without introducing foreign
DNA. This latest tool in genome editing
allows for specific genome disruption and
replacement in a flexible and simple system resulting in high specificity and low
cell toxicity. The CRISPR/Cas9 genome
editing system requires the co-expression
of a Cas9 protein with a guide RNA vector expressed from the human U6 polymerase III promoter. With the protospacer-adjacent motif (PAM—the sequence
NGG) present at the 3’ end, Cas9 will
unwind the DNA duplex and cleave both
strands upon recognition of a target sequence by the guide RNA. The functional
cassette synthesized in the rescue donor
vector can then be inserted into the unwound DNA. The repaired genome will
now express your desired sequence with
or without tags.
AMS Biotechnology
For info: +44-(0)-1235-828200
www.amsbio.com
Gene-Knockout Kits
NGS Library Preparation Kit
The epMotion automation method is now
available for the TruSeq Stranded mRNA
library preparation kit for next generation
sequencing as “Illumina Qualified”
(Illumina Qualified indicates that libraries
prepared with the epMotion method
have been shown to be comparable
to those prepared manually). For next
generation sequencing systems a variety
of sample preparation kits are available.
These kits are needed to convert either
DNA or RNA samples into sequencing
ready libraries, a procedure that includes
many steps and can be time consuming.
RNA sequencing requires additional
steps—either the depletion of unwanted
ribosomal RNA or the positive selection
of mRNA from total RNA samples. The
epMotion method can be used for the
automated construction of 8, 16, or 24
libraries starting with 100–1,000 ng of
total RNA together with Illumina’s TruSeq
Stranded mRNA kit. The overall handson time is less than 1.5 hours, while the
total run time of the entire procedure is
~11.5 hours for 24 samples.
Eppendorf
For info: +49-40-53801-729
www.eppendorf.com
New gene-knockout kits provide a
complete solution for researchers
to knock out a human gene at the
chromosomal level. This genome wide
offering provides an unprecedented
convenience for genome editing using
the CRISPR technology. OriGene has
always been focused on providing
state-of-the-art reagents of highest
quality. Its comprehensive offering of
expression cDNA clones, shRNA/siRNA,
recombinant proteins, and antibodies are
widely utilized in the biomedical research
community worldwide and are cited in thousands of publications.
A kit is provided against every human gene locus, containing two
gRNAs and a compatible donor vector with a functional cassette.
In addition to gene knockout, this kit can also be used for promoter
strength study for each gene locus. The predesigned nature of
the kit greatly reduces the researcher’s time and effort using this
platform.
OriGene Technologies
For info: 888-267-4436
www.origene.com/crispr-cas9
ChIP Sequencing Kit
Combining chromatin immunoprecipitation with massively parallel
sequencing (ChIP-seq) allows the accurate survey of interactions
between proteins and DNA, enabling the study of epigenetic marks
which is essential for a full understanding of transcriptional regulation of genes. Chromatrap is a new quicker, easier, and more efficient way of performing ChIP-seq assays. It uses discs of an inert,
porous polymer to which Protein A or
Protein G has been covalently attached
to maximize the capture efficiency of
the target chromatin/antibody complex.
Chromatrap utilizes the solid-state technology in parallel with high throughput
sequencing to deliver a precise ChIP-seq
protocol from small cell numbers and
chromatin concentrations. Specifically
adapted for greater chromatin concentrations, Chromatrap ChIP-seq now
combines the dynamic range of Chromatrap with the downstream analysis power
of deep sequencing, allowing the genome wide identification of transcription
factor binding sites and specific DNA
associated protein modifications with no
limitation in scale and resolution. Porvair Sciences
For info: +44-(0)-1978-666240
www.chromatrap.com
DNA-Sequencing Kit
The ThruPLEX DNA-sequencing (DNAseq) Kit has high multiplexing capability
with improved performance for Illumina
Next Generation Sequening (NGS) Platforms. ThruPLEX DNA-seq builds on
the innovative ThruPLEX chemistry to
generate DNA libraries with expanded
multiplexing capability and even greater
diversity with lower duplication rates.
The ThruPLEX chemistry is the only
single-tube, three-step library prep kit
for low input (0.05 ng to 50 ng) amounts,
which is especially useful for clinical
samples such plasma, other biofluids,
and FFPE samples. Kits contain up to
96 Illumina-compatible indexes predispensed and sealed in linear barcoded
microplates. ThruPLEX DNA-seq can be
used in any DNA-seq, RNA-seq, or ChIPseq application and offers robust target
enrichment performance with all of the leading platforms.
Rubicon Genomics
For info: 734-677-4845
www.rubicongenomics.com
DNA Barcodes
The NEXTflex Dual-Indexed DNA Barcodes are designed for
multiplexing of up to 192 samples for library preparation. These
barcodes are compatible with high throughput automation for increased scale and cost efficiency. The NEXTflex Dual-Indexed Barcodes can be used to multiplex genomic DNA, RNA, and ChIP sequencing libraries. The NEXTflex Dual-Indexed DNA Barcodes allow
pooling of multiple library preparations in a single flow cell lane. The
indexes are designed to be sequencing error resistant and allow for
consistent and proper differentiation between samples.
Bioo Scientific
For info: 888-208-2246
www.biooscientific.com
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Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations
are featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any
products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.
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