Cool-Season Grass Agroecozone Trial R.L. Dovel and J. Rainey' Introduction Irrigated pastures occupy over 100,000 acres in Klamath County and provide summer grazing for approximately 100,000 cattle. The currently recommended grass variety for irrigated pastures is Fawn tall fescue, a variety released in 1964. Quackgrass is also an important hay and pasture species in the area. Recently developed cultivars need to be evaluated for adaptation to the Klamath Basin. The acquisition of new germplasm from international forage breeding programs adds further impetus to the development of a forage variety screening program in the Klamath Basin. Results from a single trial location would be applicable only to that location. By establishing identical trials in different locations and documenting environmental conditions in each location, extrapolation of the data to other areas may be possible. Environmental conditions that should be monitored include soil type and pH, maximum and minimum daily temperature, precipitation, slope and aspect, and irrigation. Such a trial, called an agroecozone trial, was established on sandy loam soil at the KES in August 1994 with a complement of perennial forage grasses representing a range of forage species. A similar trial was established at Powell Butte at the Central Oregon Agricultural Research Center. Only KES data will be presented in this report. Soil samples were analyzed, and appropriate fertilizer was applied prior to planting. Seed was drilled 1/4-inch deep using a modified Kincaid plot drill. Seeding rates used in the trial are shown inTable 1. Plots were 5 x 20 feet with 3-foot wide alleyways. Plots were irrigated with solid-set sprinklers. Forages were planted in August 1994 and allowed to grow uncut through the first growing season. Three harvests per year were taken when plants began to flower in 1995 and 1996. Crops were harvested with a flail harvester. All yields are reported on a dry weight basis. Forage quality, as determined by crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), total digestible nutrients (TDN), and relative feed value (RFV), was evaluated from samples obtained at all harvests using a near-infrared spectrophotometer. Results - Procedures The trial was arranged in a randomized complete block design with four replications. 1/ 1995 Matua, an introduced Bromus species, produced the highest total season forage yield; almost 7.5 tons of dry matter /acre, which was significantly higher than all other entries (Table 2). The second highest yielding entry was Black Mountain. This perennial cereal rye, which produced forage mostly in the first cutting, had forage quality similar to hay produced by other cereal rye varieties. Other more typical grasses that produced high total forage yields include Bromar mountain brome, Fawn and Festorina tall fescue, and Potomac orchardgrass. Bromar mountain brome is a short lived perennial. It is sod forming and is used extensively in reclamation work. Associate Professor and Biological Sciences Research Technician III, respectively, Klamath Experiment Station, Klamath Falls, OR. Klamath Experiment Station 141 It would make a significant contribution to forage yields for short term pastures, but would eventually be replaced by stronger perennial grasses. Festorina is actually a Festulolium or tall fescue-perennial ryegrass cross. It is a foragetype variety that has smoother, finer leaves than traditional tall fescues. It is said to have higher forage quality than traditional tall fescues like Fawn. There was no difference in forage yield between Fawn and Festorina in 1995. Although there were no large differences in forage quality between these two varieties, there was a trend for Festorina to have higher forage quality than Fawn. Leaves of Festorina are not as rough as leaves of Fawn and it is reported to be more palatable than Fawn. More study is required to determine if tall fescueperennial ryegrass crosses are as persistent in the Klamath Basin as tall fescue. The greatest early season growth was produced by Black Mountain, a perennial cereal rye. This entry produced over 5 tons of dry matter/acre by June 22 (Table 2). The second highest early season yielding entry in the trial was Matua prairie grass. It produced significantly higher levels of forage than all other entries except the two tall fescue entries. Other entries with over 2 tons of dry matter/acre by June 22, included Fawn tall fescue, Festorina tall fescue, Potomac orchardgrass, Gala grazing brome, Bromar mountain brome, and Linn perennial ryegrass. Wana, a grazing tolerant orchardgrass, did not produce much early spring growth and only yielded 1,180 lb dry matter/acre by June 22. Palaton reed canarygrass also produced low levels of forage in the first cutting compared to its forage production in the second cutting. Black Mountain had the lowest forage quality, based on all quality parameters measured, of any entry at the first cutting (Table 3). Gala grazing brome also had lower quality than most entries at the first cutting. This variety has been noted to have lower forage quality than Matua and other Brome 142 varieties. Forage quality of Linn perennial ryegrass was also depressed in the first cutting due to advanced maturity. Although Black Mountain produced more dry matter than any other entry at the first cutting, regrowth following cutting was poor. Black Mountain only produced 970 and 730 lb dry matter/acre in the second and third cuttings, respectively (Table 2). Matua prairie grass produced almost 3 tons of dry matter/acre in the second cutting, significantly higher than all other entries in the trial (Table 2). Other high yielding varieties in the second cutting included Bromar mountain brome, Potomac and Wana orchardgrass, Palaton reed canarygrass, Festorina and Fawn tall fescue, and Gala grazing brome. Second cutting yields of both Wana orchardgrass and Palaton reed canarygrass were significantly higher than their first cutting yields. Matua prairie grass produced significantly higher third cutting forage yields than all other entries. Other entries producing above average third cutting forage yields included Fawn and Festorina tall fescue, Wana and Potomac orchardgrass, Bromar mountain brome, and Gala grazing brome. 1996 Yield trends were not the same in 1996 as 1995. Palaton, which was ranked in the lower third of the trial in 1995, was the highest yielding entry in 1996 (Table 4). Yields of Mama, which were significantly higher than any other entry in 1995, were not significantly different than yields of Palaton, Potomac, Bromar, Festorina, or Fawn. Black Mountain did not perenniate well and forage production dropped to half that of 1995. Stand thinning was also observed in Matua and Gala, and may account for the yield declines seen in these two varieties. In contrast, yields of a number of entries increased significantly over 1995 yields (Figure 1). Regar meadow brome, Oahe intermediate wheatgrass, Carlton smooth brome, and Clair Timothy are Klamath Experiment Station all species that tend to establish and reach full production potential rather slowly. The increase in forage yield in the second harvest year is due to plants maturing and increasing individual plant size. Palaton reed canary grass is a rhizomatous species that is known for low seedling vigor; however, it can be very aggressive once established. Garrison creeping foxtail is also rhizomatous and has low seedling vigor. It showed increased production in the second harvest year as well. Yields of Park Kentucky bluegrass were depressed by severe rust infestation in 1995. Rust infestation of bluegrass was light in 1996 and yields of Park more than tripled. Wana orchardgrass failed to survive the winter and much of the yield seen in 1996 is from invading Kentucky bluegrass, which is a common invasive species in irrigated pastures in the Klamath Basin. Time of production may also be an important consideration in addition to total production. Oahe produced the highest yield at the first cutting but very low yields in the second cutting. Forage yield at the third cutting recovered to levels near those of the first. Other entries with relatively high first cutting, low second cutting, and high third cutting yields include Regar, Carlton, and Bromar. In contrast, Palaton tends to produce similar yields at all three cuttings, as does Matua, the tall fescues, and to a lesser degree, the orchardgrasses. Protein concentration was generally higher in 1996 than in 1995. Some entries had larger increases in protein than others, but protein concentration exceeded 11 percent for all varieties except Black Mountain, which was 9.7 percent at the first cutting (Table 5). TDN was relatively unchanged from one year to the next. TDN levels of Clair increased slightly form 1995 to 1996 and were significantly higher than other entries in the trial. TDN levels of all entries except Black Mountain exceeded 50 percent. Forage quality of Festorina was always slightly higher than Fawn, but differences were never statically significant. Discussion and Conclusions The species currently recommended for seeding long-term irrigated pastures in the Klamath Basin is tall fescue. Data from this trial tends to support that recommendation. The tall fescue entries were in the highest yielding group in 1996 and were only exceeded by Matua in 1995. They produced high forage yields throughout the growing season and did not exhibit severe heat induced yield depression. Tall fescue is a long-lived species that resists a number of environmental stresses common to the Klamath Basin. The forage quality of the tall fescue varieties in this trial was not significantly different from other species reported to be of higher forage quality, including orchardgrass and several brome species. Orchardgrass appears to be another good choice for a long-term pasture, if appropriate grazing management is practiced. Matua and Bromar appear to have great potential for shorter rotation hay fields and pastures. Despite the excellent hay yields and quality of Matua, it is not recommended for long-term pastures due to stand thinning This variety has experienced severe stand losses in the winter, probably due to fungal attack. It also will not withstand continuous grazing or prolonged flooding. Due to high production and high protein content, nitrogen fertilization requirements are higher for Matua than other grass species. Planting this variety for hay production on well drained soil should result in a highly productive stand that will last for three to five years. Commercial stands of Matua have persisted in the Klamath Basin with moderate levels of N fertilization for four to five years. Bromar is a short-lived species that is more winter hardy than Matua. Long-term data on this species in the Klamath Basin is not available. Several drought tolerant species included in this trial yielded well. Regar meadow brome, Oahe intermediate wheatgrass and Carlton smooth brome produced about 75 Klamath Experiment Station 143 percent as much dry matter as Fawn and had better forage quality. The largest yield was in the first cutting. In pastures and hay meadows that have a seasonal or unreliable irrigation supply, the use of these varieties could provide an acceptable forage yield and survive drought stress in years when they were not irrigated. Fall regrowth of both Oahe and Regar stockpiles well and can be a valuable source of fall grazing following a spring cutting of hay. Frequently inundated or wet sites may need species that are more tolerant to flooding than tall fescue. Appropriate species for such environments include reed canarygrass and meadow foxtail. Palaton and Garrison are examples of each of these species. The impressive yields produced by Palaton in the second year of the trial on well drained soil indicate that planting of this species need not be restricted to very wet sites. However, high seed costs and low seedling vigor will probably restrict the use of this species to areas where flooding tolerance is needed. Palaton has been selected for low alkaloid content to improve palatability and animal performance. Only low alkaloid varieties of reed canarygrass should be planted for hay or pasture. These findings are preliminary. The trial will be continued for at least another year and changes in stand survival and production may Occur. Table 1. Seeding rates for species included in the forage agroecozone trial at Klamath Falls, OR., August 1994. Species Bromus willdenowii B. inermus B. stamineus B. riparius B. marginatus Dactylis glomerata Festuca arundinacea Lolium perenne Poa pratensis Alopecuras arundinacea Thinopyrum intermedium Elymus lanceolatus Secale cereale Phalaris arundinacea Phleum pratense 144 Common name prairie grass smooth brome grazing brome meadow brome mountain brome orchardgrass tall fescue perennial ryegrass Kentucky bluegrass meadow foxtail intermediate wheatgrass thick spike wheatgrass perennial cereal rye reed canarygrass timothy Klamath Experiment Station Seeding Rate lb/A Kg/ha g/m 35 25 25 35 35 20 25 30 10 5 10 10 60 15 10 39.2 28.0 28.0 39.2 39.2 22.4 28.0 33.6 11.2 5.6 11.2 11.2 67.8 16.8 11.2 392 280 280 392 392 224 280 336 112 56 112 112 678 168 112 Table 2. Agroecozone 1995 Yield Summary. 1995 forage, protein, and TDN yield summary of cool-season grasses planted at Klamath Experiment Station in August 1994. Variety Species' Cut 1 Forage Yield Cut 2 Cut 3 Total Protein yield TDN yield lb/A Park Regar Bromar Carlton Gala BLG BMD BMT BSM BST 740 3710 4870 3330 4480 830 2440 4840 2450 3550 140 330 1290 210 1210 1710 6490 10990 5980 9240 270 880 1330 830 1170 1010 3670 6260 3490 4880 Matua Garrison Oahe Potomac Wana BW CFT IWG OG OG 6820 2050 3270 4780 1180 5940 1960 1980 4070 3760 2180 230 840 1200 1350 14940 4250 6090 10050 6280 1590 660 840 1270 880 8250 2540 3590 5830 3510 Black Mnt Linn Madera Palaton Fawn PCR PRG PRG RCG TF 10410 4990 3920 1730 5510 970 2260 2960 3810 3690 730 450 350 410 1480 12110 7700 7220 5940 10680 1290 800 870 880 1370 6540 4330 4300 3450 6210 Festorina Clair Critana TF TIM TSWG 5390 3360 1760 3910 2960 1870 1320 300 390 10630 5760 4030 1410 740 540 6110 3810 2260 4020 1550 27 3010 1150 27 800 400 35 7780 2060 19 430 200 33 2270 870 27 Mean LSD (0.05) CV (%) 'BLG BMD BMT BSM BST BW CFI' IWG Kentucky bluegrass meadow brome mountain brome smooth brome Bromus stamineus Bromus willdenowii creeping foxtail intermediate wheatgrass OG PCR PRG RCG TF TIM TSWG orchardgrass perennial cereal rye perennial ryegrass reed canarygrass tall fescue timothy thickspike wheatgrass Klamath Experiment Station 145 Table 2. Agroecozone 1995 Yield Summary. 1995 forage, protein, and TDN yield summary of cool-season grasses planted at Klamath Experiment Station in August 1994. Variety Species ' Cut 1 Forage Yield Cut 2 Cut 3 Total Protein yield TDN yield lb/A Park Regar Bromar Canton Gala BLG BMD BMT BSM BST 740 3710 4870 3330 4480 830 2440 4840 2450 3550 140 330 1290 210 1210 1710 6490 10990 5980 9240 270 880 1330 830 1170 1010 3670 6260 3490 4880 Matua Garrison Oahe Potomac Wana BW CFT IWG OG OG 6820 2050 3270 4780 1180 5940 1960 1980 4070 3760 2180 230 840 1200 1350 14940 4250 6090 10050 6280 1590 660 840 1270 880 8250 2540 3590 5830 3510 Black Mnt Linn Madera Palaton Fawn PCR PRG PRG RCG TF 10410 4990 3920 1730 5510 970 2260 2960 3810 3690 730 450 350 410 1480 12110 7700 7220 5940 10680 1290 800 870 880 1370 6540 4330 4300 3450 6210 Festorina Clair Critana TF TIM TSWG 5390 3360 1760 3910 2960 1870 1320 300 390 10630 5760 4030 1410 740 540 6110 3810 2260 4020 1550 27 3010 1150 27 800 400 35 7780 2060 19 430 200 33 2270 870 27 Mean LSD (0.05) CV (%) 'BLG BMD BMT BSM BST BW CFT IWG Kentucky bluegrass meadow brome mountain brome smooth brome Bromus stamineus Bromus willdenowii creeping foxtail intermediate wheatgrass 146 OG PCR PRG RCG TF TIM TSWG orchardgrass perennial cereal rye perennial ryegrass reed canarygrass tall fescue timothy thickspike wheatgrass Klamath Experiment Station Lb/ac 1.)O) co 8 R) 4s. 0 0 0 0 0 0 0. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Matua Cereal mmovuom O O CD NM= Bromar ::-..razzatzLzEom , , Fawn 0 MBIESIME11111111 Ulabiirdi,' 7.4 7.0 6 -8 Festorina 11317217.221 Potomac ummzzsitR-um 11101111111E Gala 1,e.aiimvaimag ME Linn :701L.MaZaral Madera EnElla MEN Regar 73kMCISM-'= 1=a1 Wana r3:25... maTimm21 MEM rezi Carlton raln SIM Naimalm Palaton Oahe - - Clair Garrison :.17.37M1 MENE Mr111 Critana r7TX0=4 Park !MI (0 CO 0) 0 -n O 0) 0 Table 4. Agroecozone 1996 Yield Summary. 1996 forage, protein, and TDN yield summary of cool-season grasses planted at Klamath Experiment Station in August 1994. Variety Species' Cut 1 Forage Yield Cut 2 Cut 3 Park Regar Bromar Carlton Gala BLG BMD BMT BSM BST 2550 4230 4450 4780 2040 1250 1770 2400 1460 1520 2990 1910 3450 1870 3730 6790 7910 10300 8110 7290 1150 1040 1360 1170 1220 4480 4090 5400 4390 4120 Matua Garrison Oahe Potomac Wana BSW CFT IWG OG OG 3680 2750 4980 3470 2020 3140 1640 980 2820 2940 4090 2480 1860 4790 3920 10910 6870 7820 11080 8880 1490 1170 920 1670 1520 6260 4710 3830 6270 5450 Black Mnt Linn Madera Palaton Fawn PCR PRG PRG RCG TF 4580 2000 1880 4530 3780 1340 970 1150 3830 2630 0 1980 2820 3830 3300 5920 4950 5850 12190 9710 500 950 1130 1820 1280 2250 3730 4420 6880 5190 Festorina Clair Critana TF TIM TSWG 3840 2820 2240 2680 2750 1350 3380 2300 1520 9900 7870 5110 1310 1280 890 5190 5590 3480 3370 690 14 2030 600 21 2790 1060 27 8190 1420 12 1220 310 18 4760 1170 17 Mean LSD (0.05) CV (%) 'BLG BMD BMT BSM BST BW CFT IWG 148 Kentucky bluegrass meadow brome mountain brome smooth brome Bromus stamineus Bromus willdenowii creeping foxtail intermediate wheatgrass OG PCR PRG RCG TF TIM TSWG Total lb/A Protein yield orchardgrass perennial cereal rye perennial ryegrass reed canarygrass tall fescue timothy thickspike wheatgrass Klamath Experiment Station TDN yield Table 5. Agroecozone 1996 Quality Summary. 1996 protein and TDN summary of cool-season grasses planted at Klamath Experiment Station in August 1994. Protein Cut 2 TDN 58.0 56.8 56.6 58.3 54.4 16.8 14.5 14.2 16.8 15.7 58.8 53.3 53.9 56.6 52.0 18.5 18.6 17.1 20.2 17.3 65.4 62.1 59.8 65.9 54.9 11.2 12.7 11.4 13.2 16.7 55.2 57.2 55.6 58.4 59.0 12.5 15.4 16.8 14.4 14.4 51.3 54.5 57.4 55.4 54.4 16.4 16.4 18.2 17.8 16.5 61.4 59.0 65.3 61.9 57.4 PCR PRG PRG RCG TF 9.7 13.8 13.9 14.4 11.8 49.9 56.5 58.7 57.5 56.8 16.4 15.7 15.3 14.2 15.0 59.5 55.9 55.4 53.7 55.0 16.4 16.7 17.7 16.4 62.1 61.9 63.5 63.6 TF TIM TSWG 13.0 12.7 13.3 58.2 61.9 57.0 15.8 13.9 14.4 56.6 58.0 54.4 16.3 16.7 18.7 63.3 65.7 63.2 12.9 1.1 6 57.0 1.7 2 15.1 1.1 5 55.3 1.8 2 17.4 1.0 1 62.1 1.8 2 Cut 1 Protein Variety Species'. Park Regar Bromar Carlton Gala BLG BMD BMT BSM BST 12.3 12.3 11.7 13.4 14.1 Matua Garrison Oahe Potomac Wana BW CFI' IWG OG OG Black Mnt Linn Madera Palaton Fawn Festorina Clair Critana Mean LSD (0.05) CV (%) 1 BLG BMD BMT BSM BST BW CFT IWG Kentucky bluegrass meadow brome mountain brome smooth brome Bromus stamineus Bromus willdenowii creeping foxtail intermediate wheatgrass TDN OG PCR PRG RCG TF TIM TSWG Cut 3 Protein TDN orchardgrass perennial cereal rye perennial ryegrass reed canarygrass tall fescue timothy thickspike wheatgrass Klamath Experiment Station 149
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