79 Effects of Seed Piece Treatment on Performance of A74212-1 K.A. Rykbost', J. Maxwell' and D, Beck' INTRODUCTION In commercial and research experience A74212-1 has frequently encountered stand problems due to seed piece decay. Fusarium dry rot in seed is common, particularly in tubers over 10 ounces in size. Comparisons of several different seed lots and several seed treatment materials in 1988 were inconclusive as soil conditions were favorable and seed decay did not occur, even in untreated seed highly infected with fusarium dry rot. This experiment was conducted to continue the search for control options to address what has been a serious problem with A74212-1 in commercial production. Several new or experimental formulations of fungicides were included in the study. PROCEDURES The seed source used was obtained from a grower whose seed has experienced stand problems in previous years. Fusarium dry rot was present in a significant percentage of tubers, but not as extensively as in seed used in 1988 experiments. One batch of seed was hand cut, treated with TOPS 2.5 D immediately, and stored at 50 ° F and high relative humidity for 10 days prior to planting. The remaining treatments were batch applied to fresh-cut seed and planted on the same day, May 24. Plots were two rows, 30-feet long, arranged with four replications in a randomized complete block design. Seed was spaced at 8.7 inches in 32-inch rows. Fertilizer included 600 lbs/A of 16-16-16 banded at planting and 50 lbs N/A applied as solution 32 on June 6. Vines were desiccated with diquat at 1.5 pints/A on September 12 and potatoes were harvested on October 6. All tubers from both rows of each plot were weighed in the field. Approximately 60-pound samples were stored and graded in early November. 1/ Superintendent/Associate Professor and Biological Sciences Research Technician, respectively, Klamath Experiment Station. 2/ Associate Professor, Klamath County Extension Service. 80 RESULTS AND DISCUSSION Several small commercial fields planted locally with the same seed source during the first week of May experienced severe seed breakdown problems. In each case the seed was cut, treated with TOPS2.5 D, and planted within one day of cutting time. Within a few days after planting 0.4 inches of rainfall was followed by two days with minimum air temperatures of 30 ° and 27 ° F. Two weeks after planting a majority of seed pieces were breaking down or had already disintegrated. A sample of decaying seed was assayed. The only organism isolated was Geotricum candidum, a saprophytic organism of opportunity. By early June remaining seed pieces healed and a stand of 50 to 60 percent developed into a fair crop. In the experimental field the crop was planted in moist soil one day after a 0.38-inch rain. Emergence was quite slow and final stands were less than 85 percent for all lots planted freshly cut (Table 1). Crop vigor was generally poor. In part this was due to high salt content in the soil and heavy weed competition from nightshade, which was not adequately controlled with herbicides. Ridomil-treated seed experienced minor phytotoxic injury which plants recovered from eventually, but at the expense of some yield reduction. In both 1988 and 1989 the TOPS - Rovral treatment delayed emergence and reduced final stands slightly. This product reduced yield slightly in 1988 but not in 1989. The combination of TOPS - NAA severely delayed emergence and reduced final stand and yield. TOPS 2.5 D alone produced the highest yield of US No. l's for seed cut and planted immediately. A further yield increase was observed when a ten-day suberization period was allowed after cutting and treating. Pre-cutting and suberizing seed prior to planting did not improve performance of five seed lots in a 1988 experiment. The results of this trial and the observations of a seed grower with several years of experience with A74212-1 suggest that this practice may be beneficial in situations where adverse soil conditions occur soon after planting. SUMMARY AND CONCLUSIONS The results of this experiment suggest that the TOPS 2.5 D fungicide was superior to other products tested and that pre-cutting and suberizing seed was beneficial. The TOPS - Rovral combination increased tuber size slightly due to reduced stands. TOPS-NAA produced several undesirable effects and should be avoided. Ridomil alone reduced yields slightly, a result that has been observed elsewhere. For three consecutive years serious seed decay has occurred in one or more commercial fields of A74212-1 in the Klamath Basin. Susceptibility to tuber damage at harvest and the development of fusarium dry rot in storage undoubtedly contributes to this problem. Commercial experience with A74212-1 and research with this and other selections suggest a 81 number of control strategies to improve seed quality, including: 1) control of tuber size in seed crops; 2) a minimum delay of three weeks between topkilling and harvest; 3) careful handling at harvest and storage loading; and 4) use of a fungicide at storage loading. For seed lots with the potential for seed decay problems the following practices may reduce the severity of damage: 1) pre-cutting and suberizing seed for a minimum of 10 days prior to planting; 2) avoid planting too early and exposing the crop to cold, wet soil conditions; 3) seed treatment with an effective fungicide; and 4) cutting seed to provide large, blocky pieces with a minimum of cut surface area; 5) increasing seeding rate to compensate for loss of stand. Table 1. Effects of seed treatment on A74212-1, Klamath Experiment Station, 1989. Seed Treatment 4-6oz Yield US No l's 6-10oz >10oz Total cwt/A B's Yield No 2's Total Emergence 6/20 6/28 %- - Control Gypsom TOPS 2.5 D Ridomil-Mancozeb Ridomil-Mertect 74 54 61 56 65 111 106 113 106 102 80 114 142 142 118 265 274 316 304 285 47 47 37 43 46 16 12 8 11 17 364 364 374 384 363 65 64 64 59 69 81 80 81 80 82 TOPS-Rizolex TOPS-Rovral TOPS-NAA Ridomil TOPS 2.5 D-PreCut 67 49 40 52 97 107 94 47 96 131 123 131 110 93 125 297 274 197 240 353 49 36 15 39 63 20 14 18 10 26 407 359 279 307 460 72 54 25 56 72 83 69 47 78 89 Avg. CV (%) LSD(.05)_ 61 40 36 101 41 61 118 45 NS 280 31 127 42 29 18 15 78 17 366 28 151 60 77
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